Human CXCR4 knock-in design and confirmation of human CXCR4 expression in peripheral blood cells of heterozygous (HuCXCR4KI/WT) and homozygous (HuCXCR4KI) mice.

<p><b>A</b>, Schematic of genetic targeting strategy for knock-in of full length human CXCR4 coding region. <b>B</b>, Flow cytometric analysis of peripheral blood leukocytes from littermate mice with antibodies specific for human CXCR4.</p

The number of imported cases of infectious diseases in Guangzhou, 2005–2019.

The number of imported cases of infectious diseases in Guangzhou, 2005–2019.</p

Demographic characteristics of imported cases of acute infectious diseases in Guangzhou, China, 2005–2019.

Demographic characteristics of imported cases of acute infectious diseases in Guangzhou, China, 2005–2019.</p

The geographic distribution of imported cases and population density by district in Guangzhou, 2005–2019.

Basemap shapefile’s map content from National Earth System Science Data Center, National Science & Technology Infrastructure of China (http://www.geodata.cn), approval number 272148515751668.</p

The distribution of imported acute infectious diseases in Guangzhou, 2005–2019.

The distribution of imported acute infectious diseases in Guangzhou, 2005–2019.</p

Expression of human CXCR4 mRNA is detectable in various organs of HuCXCR4KI mice.

<p>Relative expression levels of human CXCR4 mRNA in whole organs of HuCXCR4KI male mice, as detected by RT-qPCR (Taqman). Equal amounts of total RNA were retro-transcribed and the levels of mouse Rpl19 transcript were used as a normalization control.</p

The distribution of original countries of all imported cases in Guangzhou, 2005–2019.

(XLSX)</p

Proportions of dengue, malaria, influenza A (H1N1)pdm09 and other imported cases of acute infectious diseases in Guangzhou, 2005–2019.

Dengue was the most commonly imported cases with an increasing proportion over time, followed by malaria. The influenza A (H1N1)pdm09 cases accounted for the largest proportion of imported cases in 2009.</p

Survey of human CXCR4 expression in TMA of human specimens of normal appearance and in corresponding tissues of homozygous HuCXCR4KI mice.

<p>Survey of human CXCR4 expression in TMA of human specimens of normal appearance and in corresponding tissues of homozygous HuCXCR4KI mice.</p

Normal blood cell counts in peripheral blood and normal CXCL12 levels in serum indicate proper CXCL12/CXCR4 signaling in HuCXCR4KI mice.

<p>Each symbol represents the result from an individual mouse. <b>A</b>, Flow cytometric analysis of total leukocyte (WBCs) and leukocyte subset counts in peripheral blood of HuCXCR4KI and WT control littermate mice. Cell counts were estimated with absolute counting beads, as described in the methods section. WBCs were defined as CD45⁺ blood cells. WBC subsets were defined based on cell surface marker expression, as follows: monocytes (CD45⁺CD11b⁺CD115⁺Ly6G^-), neutrophils (CD45⁺CD11b⁺CD115^-Ly6G⁺), T cells (CD45⁺CD11b^-Thy1.2⁺), B cells (CD45⁺CD11b^-CD19⁺), NK cells (CD45⁺CD11b^-CD49b⁺). <b>B</b>, Detection by ELISA of CXCL12 levels in the serum of HuCXCR4KI and WT control littermate mice; n.s. = non-significant (unpaired Student’s <i>t</i>-test).</p