MicroRNA-886 suppresses osteosarcoma cell proliferation and its maturation is suppressed by long non-coding RNA OXCT1-AS1

This study aimed to investigate the roles of microRNA-886 (miR-886) and long non-coding RNA (lncRNA) OXCT1-AS1 in osteosarcoma (OS). We predicted that they might interact with each other. The expression of OXCT1-AS1 and miR-886 (mature and premature) in osteosarcoma and paired non-tumor tissues from 66 OS patients was negatively correlated. Overexpression and silencing assays showed that OXCT1-AS1 suppresses miR-886 maturation. RNA–RNA pulldown and subcellular fractionation assays demonstrated the direct interaction between OXCT1-AS1 and miR-886. BrdU proliferation assays revealed that OXCT1-AS1 promoted OS cell proliferation, and miR-886 reduced the enhancing effects of OXCT1-AS1 on OS cell proliferation. Western blot showed that OXCT1-AS1 had no effects on the levels of epithelial–mesenchymal transition biomarkers. Overall, OXCT1-AS1 suppresses miR-886 maturation to promote OS cell proliferation.</p

Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A

Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude mice to observe tumor growth in vivo. The interaction circ_0000654, miR-149-5p, and INHBA was validated. circ_0000654 and INHBA were up-regulated but miR-149-5p was down-regulated in GC. circ_0000654 absorbed miR-149-5p to target INHBA. Silencing circ_0000654inhibited the progress of GC cell biology. Oppositely, restoring circ_0000654 enhanced the growth of GC cells. Inhibiting miR-149-5p rescued down-regulated circ_0000654-induced anti-tumor effect on GC. circ_0000654 silence or miR-149-5p overexpression limited the growth of GC tumors in vivo. Obviously, circ_0000654 facilitates the growth of GC cells through absorbing miR-149-5p to up-regulate INHBA.</p

Data_Sheet_1_The Risk of Type 2 Diabetes and Coronary Artery Disease in Non-obese Patients With Non-alcoholic Fatty Liver Disease: A Cohort Study.docx

Background: Non-alcoholic fatty liver disease (NAFLD) is not uncommon in non-obese subjects, referred to as non-obese NAFLD. It is not fully determined whether non-obese NAFLD is associated with increased risks of type 2 diabetes (T2D) and coronary artery disease (CAD) in Chinese. This study aimed to examine the association between NAFLD and risks of T2D and CAD in a non-obese Chinese population.Methods: The present cohort study included two stages. In the first cross-sectional study, 16,093 non-obese subjects with a body max index (BMI) 2 were enrolled from The Second Xiangya Hospital, China, from 2011 to 2014. Hepatic steatosis was evaluated by ultrasonography examination. Logistic regression analyses were used to examine the association of non-obese NAFLD with T2D and CAD at baseline. In the subsequent 5-year follow-up study, 12,649 subjects free of T2D and CAD at baseline were included, and the incidence of T2D and CAD were observed. Cox proportional hazard regression analyses were performed to determine the risk of incident T2D and CAD with NAFLD.Results: At baseline, the prevalence of NAFLD, T2D and CAD were 10.7% (1,717/16,093), 3.3% (529/16,093) and 0.7% (113/16,093), respectively. The univariate logistic regression analyses showed NAFLD associated with both T2D and CAD. Moreover, in a multivariate logistic regression model, NAFLD remained independently associated with T2D (OR: 2.7, 95% CI: 2.2–3.3, p Conclusions: NAFLD was an independent risk factor for T2D in non-obese subjects. However, no significant association was observed between non-obese NAFLD and incident CAD after adjusting other traditional cardiovascular risk factors, suggesting these factors might mediate the increased incidence of CAD in non-obese NAFLD patients.</p

1,4-Hydroboration Reactions of Electron-Poor Aromatic Rings by N‑Heterocyclic Carbene Boranes

Reactions of N-heterocyclic carbene boranes (NHC-boranes) with electron-poor aromatic rings under photoredox conditions provide dearomatized 3-NHC-boryl-1,5-cycohexadienes, which are formally products of 1,4-hydroboration reactions. When regioisomers are possible, the more crowded (doubly ortho-substituted) product is formed preferably or exclusively. The mechanism may involve oxidation of the NHC-borane to an NHC-boryl radical, reduction of the electron-poor aromatic ring to a radical anion, coupling of the radical and the radical anion to give a cyclohexadienyl anion, and finally regioselective protonation

Ring-Opening Reactions of NHC-Boriranes with In Situ Generated HCl: Synthesis of a New Class of NHC-Boralactones

The first ring-opening reactions of ligated boriranes (boracyclopropanes) are described. Treatment of readily available NHC-boriranes bearing ester substituents on the borirane ring with HCl provides stable γ-NHC-bora-γ-lactones in isolated yields ranging from 40% to 73%. The reactions occur through 1,3-addition of HCl across a B–C bond of the NHC-borirane to form a ring-opened NHC-boryl chloride, followed by lactonization with chloride displacement. Experimental evidence suggests that both the borirane ring-opening reaction and the boralactonization reaction occur with inversion at boron

Ring-Opening Reactions of NHC-Boriranes with In Situ Generated HCl: Synthesis of a New Class of NHC-Boralactones

The first ring-opening reactions of ligated boriranes (boracyclopropanes) are described. Treatment of readily available NHC-boriranes bearing ester substituents on the borirane ring with HCl provides stable γ-NHC-bora-γ-lactones in isolated yields ranging from 40% to 73%. The reactions occur through 1,3-addition of HCl across a B–C bond of the NHC-borirane to form a ring-opened NHC-boryl chloride, followed by lactonization with chloride displacement. Experimental evidence suggests that both the borirane ring-opening reaction and the boralactonization reaction occur with inversion at boron

Ring-Opening Reactions of NHC-Boriranes with In Situ Generated HCl: Synthesis of a New Class of NHC-Boralactones

The first ring-opening reactions of ligated boriranes (boracyclopropanes) are described. Treatment of readily available NHC-boriranes bearing ester substituents on the borirane ring with HCl provides stable γ-NHC-bora-γ-lactones in isolated yields ranging from 40% to 73%. The reactions occur through 1,3-addition of HCl across a B–C bond of the NHC-borirane to form a ring-opened NHC-boryl chloride, followed by lactonization with chloride displacement. Experimental evidence suggests that both the borirane ring-opening reaction and the boralactonization reaction occur with inversion at boron

Ring-Opening Reactions of NHC-Boriranes with In Situ Generated HCl: Synthesis of a New Class of NHC-Boralactones

The first ring-opening reactions of ligated boriranes (boracyclopropanes) are described. Treatment of readily available NHC-boriranes bearing ester substituents on the borirane ring with HCl provides stable γ-NHC-bora-γ-lactones in isolated yields ranging from 40% to 73%. The reactions occur through 1,3-addition of HCl across a B–C bond of the NHC-borirane to form a ring-opened NHC-boryl chloride, followed by lactonization with chloride displacement. Experimental evidence suggests that both the borirane ring-opening reaction and the boralactonization reaction occur with inversion at boron

Ring-Opening Reactions of NHC-Boriranes with In Situ Generated HCl: Synthesis of a New Class of NHC-Boralactones

The first ring-opening reactions of ligated boriranes (boracyclopropanes) are described. Treatment of readily available NHC-boriranes bearing ester substituents on the borirane ring with HCl provides stable γ-NHC-bora-γ-lactones in isolated yields ranging from 40% to 73%. The reactions occur through 1,3-addition of HCl across a B–C bond of the NHC-borirane to form a ring-opened NHC-boryl chloride, followed by lactonization with chloride displacement. Experimental evidence suggests that both the borirane ring-opening reaction and the boralactonization reaction occur with inversion at boron