Epidemiological characteristics of nine <i>bla</i>_NDM-1-positive bacteria.

<p>Epidemiological characteristics of nine <i>bla</i>_NDM-1-positive bacteria.</p

EcoRI-digestion profiles and Southern blotting results of extracted plasmids.

<p>(A) Plasmid digested with EcoRI and then run in gel. (B) Southern blot hybridization results of digested plasmid DNA. M: λDNA\HindIII. Lane 1∼7: <i>K. pneumoniae</i> M194, <i>Enterobacter cloacae</i> Q297, <i>Enterobacter aerogenes</i> Q442, <i>Citrobacter freundii</i> X122, <i>A. calcoaceticus</i> G113, <i>A. calcoaceticus</i> X231 and <i>K. pneumoniae</i> U091, respectively.</p

The MICs of <i>bla</i>_NDM-1-positive bacteria.

<p>PRL: Piperacillin. CTX: Cefotaxime. CRO: Ceftriaxone. CAZ: Ceftazidime. SCF: Cefoperazone/sulbactam2:1. FEP: Cefepime. ATM: Aztreonam. IMP: Imipenem. MEM: Meropenem. PAN: panipenem. ETP: ertapenem. GEN: Gentamicin. AMK: Amikacin. TCY: tetracycline. MNO: Minocycline. TGC: Tigecycline. CIP: ciprofloxacin. LVP: Levofloxacin. NIT: Nitrofurantoin. POL: Polymyxin B. POS: phosphonomycin. USA-FDA breakpoint was applied for tigecycline (S: ≤2 mg⋅L⁻¹; R: ≥8 mg⋅L⁻¹) in both <i>Enterobacteriaceae</i> and <i>A. calcoaceticus</i>.</p><p>The MICs of <i>bla</i>_NDM-1-positive bacteria.</p

Result of S1-PFGE and Southern blot hybridization.

<p>(A) The left dark background figure shows the results of S1-PFGE, while the right light background shows the Southern blot hybridization. M: Low Range PFG Marker. Lane 1∼6: <i>A. calcoaceticus</i> G113, <i>K. pneumoniae</i> M186, <i>K. pneumoniae</i> M187, <i>K. pneumoniae</i> M194, <i>K. pneumoniae</i> U091 and <i>Citrobacter freundii</i> X122, respectively. (B) The left dark background figure shows the results of S1-PFGE, while the right light background shows the Southern blot hybridization. M: Low Range PFG Marker. Lane 1∼3: <i>Enterobacter cloacae</i> Q297, <i>Enterobacter aerogenes</i> Q442 and <i>A. calcoaceticus</i> X231, respectively.</p

PFGE profiles of <i>Salmonella</i> serotype <i>Braenderup</i> strain (H9812), and four <i>K. pneumoniae</i> and two <i>A. calcoaceticus</i> isolates.

<p>(A) PFGE profiles of H9812 and four <i>K. pneumoniae</i> isolates. Lane 1: <i>Salmonella</i> serotype <i>Braenderup</i> strain (H9812), as the marker. Lane 2∼5: four <i>K. pneumoniae</i> isolates (M186, M187, M194, U091, respectively). (B) PFGE profiles of H9812 and two <i>A. calcoaceticus</i> isolates. Lane 1: <i>Salmonella</i> serotype <i>Braenderup</i> strain (H9812), as the marker. Lane 2∼3: two <i>A. calcoaceticus</i> isolates, G113 and X231, respectively.</p