Additional file 4 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 4: Supplemental Table 2. GS biosynthesis and degradation related genes in Chinese kale. The value of log2 (B/A) reflects the comparison of gene expression level in B compared with the A, greater than 0 means that the gene expression level of the treatment group is up-regulated, and less than 0 means that it is down-regulated. A represents the control group, B represents the treatment group. When log2(B/A) > 2, it means significantly regulated

Additional file 2 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 2 Supplemental Fig. 2. KEGG classification of DEGs. The X axis is the number of genes ann:otated to a KEGG pathway category, and the Y axis is the KEGG pathway category

Additional file 1 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 1: Supplemental Fig. 1. GO classification of differential expressed genes (DEGs). The X axis represents the number of genes annotated to the GO Terms, and the Y axis represents the classification of GO

Additional file 5 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 5: Supplemental Table 3. GS transporter related genes in Chinese kale. The value of log2 (B/A) reflects the comparison of gene expression level in B compared with the A, greater than 0 means that the gene expression level of the treatment group is up-regulated, and less than 0 means that it is down-regulated. A represents the control group, B represents the treatment group. When log2(B/A) > 2, it means significantly regulated

Additional file 3 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 3: Supplemental Table 1. Glucosinolate profiles and their content in the seed and corresponding silique walls at different stages

Additional file 6 of Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds

Additional file 6: Supplemental Table 4. MYB transcriptional factors related genes to GS biosynthesis in Chinese kale. The value of log2 (B/A) reflects the comparison of gene expression level in B compared with the A, greater than 0 means that the gene expression level of the treatment group is up-regulated, and less than 0 means that it is down-regulated. A represents the control group, B represents the treatment group. When log2(B/A) > 2, it means significantly regulated

Image_1_Integration of Small RNA and Transcriptome Sequencing Reveal the Roles of miR395 and ATP Sulfurylase in Developing Seeds of Chinese Kale.tif

Seed development is closely related to plant production and reproduction, and MicroRNAs (miRNA) is widely involved in plant development including seed development. Chinese kale, as a Brassicaceae vegetable, mainly depends on seed for proper reproduction. In the present study, Chinese kale seed and silique at different stages were selected to establish small RNA (sRNA) libraries including silique wall sRNA libraries at torpedo-embryo stage (PC), silique wall sRNA libraries at cotyledonary-embryo stage (PD), seed sRNA libraries at torpedo-embryo stage (SC), and seed sRNA libraries at cotyledonary-embryo stage (SD). The results showed that miRNA expressed differentially in the seeds and corresponding siliques at different stages. To further clarify the functional mode of miRNA in the process of seed development, Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis was performed on target genes of the differentially expressed miRNAs, and these target genes were mainly enriched in plant hormone signal transduction, primary and secondary metabolic pathways. After joint analysis with the transcriptome change of the corresponding period, miR156-SPL10/SPL11, miR395-APS3, and miR397-LAC2/LAC11 modules were identified to be directly involved in the development of Chinese kale seeds. What’s more, modified 5′RLM-RACE and Agrobacteria-mediated Chinese kale transient transformation suggest miR395b_2 is involved in sulfur metabolism during seed development by regulating its target gene APS3.</p