Additional file 1 of Characterization of the REVEILLE family in Rosaceae and role of PbLHY in flowering time regulation

Additional file 1: Fig. S1. (a) Coding sequence of PbLHY. (b) Alignment of PbLHY and AtCCA1/AtLHY protein sequences. The red box indicates the MYB domain. The blue backgrounds correspond to the percent identity of the multiple alignment. Fig. S2. Alignment of conserved domains from PbRVE proteins. (a) Alignment of MYB domains from ten PbRVEs and eleven AtRVEs. Red box indicates MYB domain, and blue box indicates SHAQK(Y/F) F sequence. (b) Alignment of LCL domains from subfamily II members. The red box indicates the MYB domain. The green box indicates the LCL domain. The blue backgrounds correspond to the percent identity of the multiple alignment. Fig. S3. The motif details of ten conserved domains identified in RVE proteins and the logos of these domains created using the MEME. Amino acids are expressed in the standard single letter code. The size of the letters at each position represents their frequency

Additional file 2 of Characterization of the REVEILLE family in Rosaceae and role of PbLHY in flowering time regulation

Additional file 2: Table S1. Characteristics of RVE family members in seven Rosaceae species. Table S2. Numbers of RVE gene pairs from different duplication events in seven Rosaceae species. Table S3. Ka and Ks calculation of duplicated RVE gene pairs in seven Rosaceae species. Table S4. The primers used in the assays

Table_1_Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco.docx

Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H2O2. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. These results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.</p

Table_2_Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco.docx

Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H2O2. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. These results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.</p