Relative expression of OBPs and CSPs genes to GAPDH in <i>Anomala corpulenta</i> (A) and the FPKM values of those genes (B).

<p>Bars denoted by the same letter indicate that there are no significant differences in gene expression in antenna (Tukey’s b P>0.05).</p

Characteristics of homology search of assembled unigenes against the nr database.

<p>(A) E-value distribution; (B) Similarity distribution; (C) Species distribution; the first hits of each unigene were used for analysis.</p

Phylogenetic tree of OBPs (A) and CSPs (B) from <i>Anomala corpulenta</i> and other Coleoptera species constructed by BEAST 2.

<p>The Obps and Csps from <i>A. corpulenta</i> were underlined. Bh: <i>Batocera horsfieldi</i>; Ha: <i>Harmonia axyridis</i>; Ho: <i>Holotrichia oblita</i>; Ma: <i>Monochamus alternatus</i>; Pd: <i>Phyllopertha diversa</i>; Tc: <i>Tribolium castaneum</i>; Dp: <i>Dendroctonus ponderosae</i>.</p

Alignment of amino acid sequences of OBPs (A) and CSPs (B) from <i>A. corpulenta</i>.

<p>Conserved residues were highlighted in gray and conserved cysteines were marked with “*” below the alignment.</p

FPKM values versus different lengths of unigenes.

<p>FPKM values versus different lengths of unigenes.</p

Unigene hits against the three main public databases.

<p>Unigene hits against the three main public databases.</p

Unigenes related to chemodetection.

<p>Number of sequences obtained in this study with hits to the corresponding proteins in the NCBI nr database.</p><p>Unigenes related to chemodetection.</p

11,154 unigenes were hits in Gene Ontology (GO) classification.

<p>The results are assigned in three main categories: biological process, cellular component and molecular function. The x-axis indicates the sub-categories and the y-axis indicates the number of unigenes in that category.</p

The length distribution of contigs and unigenes.

<p>The length distribution of contigs and unigenes.</p

Identified and sequence confirmed OBP and CSP unigenes.

<p>Identified and sequence confirmed OBP and CSP unigenes.</p