Perinatal mortality in gestational hypertensive versus non-hypertensive twin pregnancies, U.S. matched multiple birth data 1995–2000.

<p>HR = Hazard ratio; CI =  confidence interval.</p><p>*Hazard ratios adjusted for maternal race, marital status, age, education, parity, smoking, other maternal major illnesses, fetal sex, mode of delivery and twin-cluster level dependence in Cox regression models.</p><p>**There were a significant number of perinatal deaths with missing birth weights.</p>t<p> Gestational age group-specific mortality rates and hazard ratios were calculated using the number of foetuses at risk and the number of perinatal deaths in the time interval specified.</p

Stillbirth in gestational hypertensive versus non-hypertensive twin pregnancies, U.S. matched multiple birth data 1995–2000.

<p>HR = Hazard ratio; CI =  confidence interval.</p><p>*Hazard ratios adjusted for maternal race, marital status, age, education, parity, smoking, other maternal major illnesses, fetal sex, mode of delivery and twin-cluster level dependence in Cox regression models.</p><p>**There were a significant number of stillbirths with missing birth weights.</p>t<p> Gestational age group-specific mortality rates and hazard ratios were calculated using the number of foetuses at risk and the number of stillbirths in the time interval specified.</p

Survival probabilities during the perinatal period (from 20 weeks gestation to 4 weeks postpartum) in gestational hypertensive vs. non-hypertensive twin pregnancies.

<p>Survival probabilities during the perinatal period (from 20 weeks gestation to 4 weeks postpartum) in gestational hypertensive vs. non-hypertensive twin pregnancies.</p

Maternal, pregnancy and newborn characteristics in gestational hypertensive versus non-hypertensive twin pregnancies in the study population, U.S. 1995–2000.

<p>Data presented are n (%). P values are from Chi-square tests for differences between diabetic and non-diabetic pregnancies. *SGA = Small-for-gestational-age <10^th percentile, LGA = large-for-gestational-age >90^th percentile, according to birth weight percentiles in non-malformation births to non-smoking mothers in the study cohort.</p>c<p> One or more of the following conditions: diabetes, heart disease, acute or chronic lung disease, renal disease, genital herpes and RH sensitization.</p>t<p> There were significant numbers of missing value (>10%) for smoking (n = 49494 mothers) (17.8%) and mode of delivery (101368 mothers) (36.4%). The numbers of missing for other variables were: race 0, marital status 705 (0.3%) mothers, age 0, education 3389 (1.2%) mothers, parity 12 (0.0%), other maternal illness 20721 (7.4%) mothers, preterm birth 0, low birth weight, SGA or LGA 8615 (1.5%) newborns. The rates for smoking and caesarean section, SGA, et al. are based on births with non-missing information.</p

Identification and Analysis of Differential miRNAs in PK-15 Cells after Foot-and-Mouth Disease Virus Infection

<div><p>The alterations of MicroRNAs(miRNAs) in host cell after foot-and-mouth disease virus (FMDV) infection is still obscure. To increase our understanding of the pathogenesis of FMDV at the post-transcriptional regulation level, Solexa high-throu MicroRNAs (miRNAs) play an important role both in the post-transcriptional regulation of gene expression and host-virus interactions. Despite investigations of miRNA expression ghput sequencing and bioinformatic tools were used to identify differentially expressed miRNAs and analyze their functions during FMDV infection of PK-15cells. Results indicated that 9,165,674 and 9,230,378 clean reads were obtained, with 172 known and 72 novel miRNAs differently expressed in infected and uninfected groups respectively. Some of differently expressed miRNAs were validated using stem-loop real-time quantitative RT-PCR. The GO annotation and KEGG pathway analysis for target genes revealed that differently expressed miRNAs were involved in immune response and cell death pathways.</p></div

Part gene ontology classification annotated by BGI WEGO for predicted target genes.

<p>The figure shows partial GO enrichment for the predicted target genes in molecular function, cellular component and biological processes.</p

The endogenous novel miRNA exhibited significant base bias especially in 20, 22 and 24

<p>The endogenous novel miRNA exhibited significant base bias especially in 20, 22 and 24</p

Length distribution and abundance of sequences in peak and late lactation.

<p>Sequence length distribution of clean reads based on the abundance and distinct sequences.</p

Summary of miRNA primers used in real-time qRT-PCR.

<p>Summary of miRNA primers used in real-time qRT-PCR.</p

Comparison of expression levels of known miRNAs (A) and novel miRNAs (B) in infected and uninfected groups.

<p>The X- and Y-axes show the expression levels of miRNAs in the two samples. The red points represent miRNAs with ratios above 2; the blue points represent miRNAs with ratios between 1/2 and 2; the green points represent miRNAs with ratios under 1/2. Ratios = miRNA expression levels in infection/miRNA expression levels in uninfected group.</p