China Climate Change News Indices

The daily climate change news indices for China from 2010 to 2023 are provided. Initially, based on nearly 4.9 million news articles published by nine Chinese newspapers (People's Daily, Guangming Daily, Xinhua Daily Telegraph, China News Service, Science and Technology Daily, Science Times, China Energy News, China Environment News, and Global Times) from January 2010 to November 2022, the indices were constructed using the Latent Dirichlet Allocation (LDA) model, encompassing five distinct themes: natural disasters, climate governance, energy transition, climate cooperation, and climate communication. Subsequently, the indices were updated to 2023 utilizing a naive Bayes model.Cite: Ma, D., Zhang, Y., Ji, Q., Zhao, W. L., & Zhai, P. (2024). Heterogeneous impacts of climate change news on China's financial markets. International Review of Financial Analysis, 91, 103007.</p

Supplementary document for A technique for enhancing the accuracy of the Rayleigh- Sommerfeld convolutional diffraction through the utilization of independent spatial sampling - 6681433.pdf

Description of BLFFT improvement

DataSheet1_Genomic profiling of WRKY transcription factors and functional analysis of CcWRKY7, CcWRKY29, and CcWRKY32 related to protoberberine alkaloids biosynthesis in Coptis chinensis Franch.ZIP

Coptis chinensis Franch. (Huanglian in Chinese) is an important economic crop with medicinal value. Its rhizome has been used as a traditional herbal medicine for thousands of years in Asia. Protoberberine alkaloids, as the main bioactive component of Coptis chinensis, have a series of pharmacological activities. However, the protoberberine alkaloids content of C. chinensis is relatively low. Understanding the molecular mechanisms affecting the transcriptional regulation of protoberberine alkaloids would be crucial to increase their production via metabolic engineering. WRKY, one of the largest plant-specific gene families, regulates plant defense responses via the biosynthesis of specialized metabolites such as alkaloids. Totally, 41 WRKY transcription factors (TFs) related to protoberberine alkaloid biosynthesis were identified in the C. chinensis genome and classified into three groups based on phylogenetic and conserved motif analyses. Three WRKY genes (CcWRKY7, CcWRKY29, and CcWRKY32) may regulate protoberberine alkaloid biosynthesis, as suggested by gene-specific expression patterns, metabolic pathways, phylogenetic, and dual-luciferase analysis. Furthermore, the CcWRKY7, CcWRKY29, and CcWRKY32 proteins were specifically detected in the nucleus via subcellular localization. This study provides a basis for understanding the regulatory mechanisms of protoberberine alkaloid biosynthesis and valuable information for breeding C. chinensis varieties.</p

Highly Catalytic CoP@N, P‑Codoped Porous Carbon Synthesized by a Supramolecular Gel and Salt Template Method for Li–S Batteries

Lithium polysulfides (LiPSs) shuttling effect is the main problem to be solved for cathode materials of lithium–sulfur batteries. The adsorption and catalytic conversion of LiPSs by host materials have become the main focus of cathode materials. In this work, transition metal phosphides are combined with three-dimensional carbon nanosheets to form an efficient and stable sulfur host material. The designed composite material is effective in solving the problems of slow reaction kinetics of Li–S batteries and LiPSs shuttling. Here, through the supramolecular self-assembly process of melamine and phytic acid, combined with soluble salt template technology, N- and P-codoped three-dimensional hierarchical porous carbon materials with uniformly dispersed CoP nanoparticles were efficiently synthesized. The catalytic effect of CoP nanoparticles improves the reaction kinetics effectively of LiPS conversion. The strong polarity of CoP nanoparticles is beneficial to the adsorption of polysulfide ions. Moreover, the high specific area provides more LiPS adsorption sites, and the doping of N and P heteroatoms further increases the active sites of the composites. The experimental results and theoretical calculations show that the introduction of CoP promotes the conversion of LiPSs and accelerates the nucleation rate of Li2S, thereby improving the electrochemical performance of the composite as a sulfur host for lithium–sulfur batteries

Identification and Functional Characterization of a Novel Sinapyl Alcohol Acyltransferase from Euphorbia lathyris L.

Methoxyeugenol is a phenylpropene compound derived from plants and has various bioactivities. The chemical synthesis of methoxyeugenol is accompanied by pollution issues, whereas extraction from plants is associated with problems such as low yield and high cost. The production of methoxyeugenol can be effectively addressed through an enzymatic approach. In this study, the acyltransferase genes of Euphorbia lathyris L. were screened by homologous alignment of the transcriptome data of E. lathyris in the late growth stage and the acyltransferase genes of the closely related plant species. The results showed that ElBAHD10 had the closest relationship with earlier reported ScCFAT and PhCFAT, which were found to catalyze the reaction of coniferyl alcohol to generate coniferyl acetate. The ElBAHD10 gene was successfully cloned from E. lathyris and subsequently expressed in Escherichia coli. The purified protein ElBAHD10 catalyzed the reaction of sinapyl alcohol with acetyl CoA and cinnamoyl CoA to form sinapyl acetate and sinapyl cinnamate, respectively. In contrast, the crude ElBAHD10 protein could catalyze sinapyl alcohol to directly generate methoxyeugenol. The recombinant E. coli strain expressing ElBAHD10 produced methoxyeugenol through whole-cell transformation. This study provides insights and lays the foundation for methoxyeugenol production through biosynthetic approaches

Integrated Transcriptomic and Proteomic Analysis Identifies Novel Regulatory Genes Associated with Plant Growth Regulator-Induced Astringency in Grape Berries

Astringency influences the sensory characteristics and flavor quality of table grapes. We tested the astringency sensory attributes of berries and investigated the concentration of flavan-3-ols/proanthocyanidins (PAs) in skins after the application of the plant growth regulators CPPU and GA3 to the flowers and young berries of the “Summer Black” grape. Our results showed that CPPU and GA3 applications increase sensory astringency perception scores and flavan-3-ol/proanthocyanidin concentrations. Using integrated transcriptomic and proteomic analysis, differentially expressed transcripts and proteins associated with growth regulator treatment were identified, including those for flavonoid biosynthesis that contribute to the changes in sensory astringency levels. Transient overexpression of candidate astringency-related regulatory genes in grape leaves revealed that VvWRKY71, in combination with VvMYBPA1 and VvMYC1, could promote the biosynthesis of proanthocyanidins, while overexpression of VvNAC83 reduced the accumulation of proanthocyanidins. However, in transient promoter studies in Nicotiana benthamiana, VvWRKY71 repressed the promoter of VvMYBPA2, while VvNAC83 had no significant effect on the promoter activity of four PA-related genes, and VvMYBPA1 was shown to activate its own promoter. This study provides new insights into the molecular mechanisms of sensory astringency formation induced by plant growth regulators in grape berries

Integrated Transcriptomic and Proteomic Analysis Identifies Novel Regulatory Genes Associated with Plant Growth Regulator-Induced Astringency in Grape Berries

Astringency influences the sensory characteristics and flavor quality of table grapes. We tested the astringency sensory attributes of berries and investigated the concentration of flavan-3-ols/proanthocyanidins (PAs) in skins after the application of the plant growth regulators CPPU and GA3 to the flowers and young berries of the “Summer Black” grape. Our results showed that CPPU and GA3 applications increase sensory astringency perception scores and flavan-3-ol/proanthocyanidin concentrations. Using integrated transcriptomic and proteomic analysis, differentially expressed transcripts and proteins associated with growth regulator treatment were identified, including those for flavonoid biosynthesis that contribute to the changes in sensory astringency levels. Transient overexpression of candidate astringency-related regulatory genes in grape leaves revealed that VvWRKY71, in combination with VvMYBPA1 and VvMYC1, could promote the biosynthesis of proanthocyanidins, while overexpression of VvNAC83 reduced the accumulation of proanthocyanidins. However, in transient promoter studies in Nicotiana benthamiana, VvWRKY71 repressed the promoter of VvMYBPA2, while VvNAC83 had no significant effect on the promoter activity of four PA-related genes, and VvMYBPA1 was shown to activate its own promoter. This study provides new insights into the molecular mechanisms of sensory astringency formation induced by plant growth regulators in grape berries