Aktivitas Antibakteri Ekstrak Etanol Kayu Nangka (Artocarpus Heterophylla Lmk.) Terhadap Bacillus Subtilis Dan Escherichia Coli

Research on antibacterial activity of jackfruit wood (A. heterophylla Lmk.) ethanolic extract to B. subtilis and E. coli in vitro have been done. Jackfruit wood extraction was done with maceration method using ethanolic solvent 70%. Antibacterial activity test used agar diffution method. There were tested 5 groups of serial extract concentration 1%, 10% and 100%; positive control (tetracycline HCl) and solvent control/ negative control Dimethylsulfoxide (DMSO 10%). The result showed that ethanolic extract of jackfruit wood didn't have antibacterial activity until the highest concentration. Thin Layer Chromatography (TLC) test showed that ethanolic extract of jackfruit wood contain flavonoid and alkaloid. Keywords: antibacterial, ethanolic extract of jackfruit wood (A. heterophylla Lmk.), Bacillus subtilis, Escherichia coli

Daya Repelan Dan Uji Iritasi Formula Lotion Ekstrak Etanol Daun Sirih (Piper Betle Linn) Dengan Variasi Basis Lanolin Terhadap Nyamuk Aedes Aegypti

Dengue hemorrhagic fever (DHF) which is transmitted through the bite of mosquito Aedes aegypti and Aedes albopictus females still becomes a health problem, especially in Indonesia. Repellant is used to avoid interference or bite of the Aedes aegypti mosquito. The purposes of this research are to determine the repellency of the ethanolic extract of betel leaf lotion with lanolin variation as a basis toward Aedes aegypti, to determine whether the ethanolic extract of piper betel leaves lotion with lanolin variation as a basis irritate the test animals or not, and to compare the influence of lanolin concentration toward physical characteristic of lotion. Piper betel leaves extracted with 96% ethanol, using reflux. The ethanolic extract of piper betel leaves used in the formula lotion is 5%. This lotion was made into 3 formula with lanolin concentration difference 3%, 4% and 5%. Lotion ethanolic extract of piper betel leaves had been made then evaluated physically, repellency and irritation. Dispersive and viscosity of the lotion will determine the convenience of the use by human. Differences of lanolin concentration in each formula lotion, greatly affect the viscosity, dispersive, adhesion and repellency each formula. Repellency lotion ethanolic extract of piper betel leaves is 88% in 11 minutes. The primary irritation index lotion ethanolic extract of piper betel leaves is 0,33. Concentration of lanolin is proportional to the lotion repellant the ethanolic extract of piper betel leaves. The primary irritation index lotion ethanolic extract of piper betel leaves is 0,33 or few stimulating. Concentration of lanolin inversely to the value of dispersive, but it is directly proportional to the value of adhesion and viscosity lotion. Keywords : repellency, irritation, lotion, ethanolic extract of piper betel leaves (Piper betle Linn

Antituberculosis Activity of Brotowali (Tinospora Crispa) Extract and Fractions Against Mycobacterium Tuberculosis Using Microplate Alamar Blue Assay Method

Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) has created a need to discover a new antituberculosis drug candidate. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay (MABA) method. T. crispa extract was prepared by maceration in ethanol (96%) and antituberculosis activity was carried out using MABA method. The result of this study showed that ethanolic extract of T. crispa exhibit antituberculosis activity with minimum inhibition concentration of 12.5 mg/ml

Ekstrak Daun Cocor Bebek (Bryophyllum Pinnatum) Sebagai Pengawet Alami Pada Sediaan Sirup Herbal Tomat (Solanum Lycopersicum)

Synthetic preservatives application in foods continuously, such as formalin can cause a variety of diseases. Alternatives of antimicrobials and preservatives which have less negative impact on health becomes necessary. It can be obtained from the cocor bebek leaves which is possess antimicrobial activity. Thus can be developed as a natural preservative. This research was aimed to determine the effectiveness of the ethanol extract of cocor bebek leaves as a preservative in Tomato Syrup, and also to determine microbial contamination in tomato syrup that has added leaf extract of cocor bebek against storage temperature variations. This research method was an experimental by testing microbial contamination in tomatoes syrup by observing Total Plate Count and Fungus / Yeast Total, and Staphylococcus aureus test. The result show that extract cocor bebek with the concentration 3.2 % was able to preserve tomatoes syrup for 1 week. It could be observed from the value of total plate count, yeast total and Identification of Staphylococcus aureus. Storage at refrigerator temperature indicates a smaller value than at room temperature. Cocor bebek leaf extract with the concentration 3.2% was able to preserve syrup tomatoes herbal for 1 week. Key words: Bryophyllum pinnatum, cocor bebek, natural preservatives, tomato syrup, Solanum lycopersicum, Staphylococcus aureus

Mutagenic Effect of Ethanol Extract of Jatropha Curcas L Seed Solid Waste Obtained From Residual Fuel Vegetable Processing (Biofuel)

Jatropha curcas seed contains viscous oil that can be used for soap making, cosmetic and as biofuel. It contains phorbol ester that was toxic. Biofuel production of Jatropha curcas seed left seedcake from mechanical press process. For safety evaluation, mutagenicity test was carried out. The seedcake was extracted by maceration method at room temperature with methanol and the mutagenic effect was evaluated by Ames test against Salmonella typhimurium TA 1535 with or without S9 metabolic activator. Methanolic extract of Jatropha curcas seedcake had no mutagenicity effect against S. typhimurium TA 1535

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) has created a need to discover a new antituberculosis drug candidate. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay (MABA) method. T. crispa extract was prepared by maceration in ethanol (96%) and antituberculosis activity was carried out using MABA method. The result of this study showed that ethanolic extract of T. crispa exhibit antituberculosis activity with minimum inhibition concentration of 12.5 mg/ml