International forum. an investigation of iron status in blood donors

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Identifying SARS-CoV-2 'memory' NK cells from COVID-19 convalescent donors for adoptive cell therapy

COVID-19 disease is the manifestation of syndrome coronavirus 2 (SARS-CoV-2) infection, which is causing a worldwide pandemic. This disease can lead to multiple and different symptoms, being lymphopenia associated with severity one of the most persistent. Natural killer cells (NK cells) are part of the innate immune system, being fighting against virus-infected cells one of their key roles. In this study, we determined the phenotype of NK cells after COVID-19 and the main characteristic of SARS-CoV-2-specific-like NK population in the blood of convalescent donors. CD57+ NKG2C+ phenotype in SARS-CoV-2 convalescent donors indicates the presence of 'memory'/activated NK cells as it has been shown for cytomegalovirus infections. Although the existence of this population is donor dependent, its expression may be crucial for the specific response against SARS-CoV-2, so that, it gives us a tool for selecting the best donors to produce off-the-shelf living drug for cell therapy to treat COVID-19 patients under the RELEASE clinical trial (NCT04578210)

Factor hormonal en el aborto - Su estudio por el Urocitograma

En repetidas ocasiones se ha afirmado que la investigación del factor hormonal como causa de aborto, utilizando las titulaciones hormonales (estrógenos, progesterona), o la de sus metabolitos (pregnandiol. etc.), son técnicas de difícil aplicación, en el caso concreto de una mujer grávida que consulta por amenaza de aborto.</jats:p

4polar3D : Single molecule 3D orientation imaging of dense actin networks using ratiometric polarization splitting

Single Molecule Orientation and Localization Microscopy (SMOLM) aims at simultaneously measuring the position and orientation of single molecules, generating orientation-encoded super-resolved images by estimating both their 3D mean orientation and the extent of their angular fluctuations(wobble). Most existing SMOLM approaches rely on the engineering of single molecules’ point spread functions, which requires complex optical setups and long computational times that can be an obstacle in dense cellular environments with high detection density and challenging imaging conditions. In this work, we propose a simpler and effective method named 4polar3D, based on the estimation of single molecule intensities projected onto four polarized channels with controlled numerical apertures. This strategy enables 3D orientation measurements of single molecules in addition to their 2D localization in a fast processing step. We demonstrate that 4polar3D can resolve nanoscale molecular organization in whole cells’ crowded structures, uncovering 3D-oriented actin filament networks in densely packed lamellipodia and podosomes

IkasSasoi: movilizando la comunidad para fomentar la actividad física de la población adolescente de Irun

The adolescence is a period of particular interest to promote healthy habits, including regular physical activity. Interventions addressed to this population group have traditionally focused on the area of the school. The knowledge obtained from research in the area of promoting physical activity seems to draw multicomponent strategies as the most appropriate. IkasSasoi is a multicomponent initiative promoted by the City Council of Irun in which various municipal departments and community agents take part and which�s aim is to promote physical activity and avoidance of sedentary behavior among adolescents studying ESO in Irun. IkasSasoi includes an initial diagnosis, for which it has been administered a questionnaire on physical activity habits to 1.797 subjects and developed four focus groups. From the information gathered, the working group has validated 23 interventions, 19 of them are underway or already completed. IkasSasoi, has revealed the habits and perceptions of the teenagers and generated a consolidated intersectorial network. The program is placing adolescents on the agenda of local policies related to physical activity. The main areas for improvement relate to missed intervention�s deadlines of interventions and the unequal involvement of actors, especially in schoolsLa adolescencia es un período de especial interés para el fomento de hábitos saludables, entre ellos el de practicar actividad física con regularidad. Diversas intervenciones que han actuado sobre este grupo de población, se han centrado tradicionalmente en el ámbito del centro escolar. El conocimiento aportado por las investigaciones en el área del fomento de la actividad física parece señalar a las estrategias multicomponente como las más indicadas. IkasSasoi es una iniciativa multicomponente promovida por el Ayuntamiento de Irun en la que participan distintas áreas municipales y distintos agentes de la comunidad que persigue fomentar la actividad física y la evitación de la conducta sedentaria entre los y las escolares que cursan E.S.O. en Irun. IkasSasoi contempla un diagnóstico inicial, para lo cual se ha administrado un cuestionario sobre hábitos de actividad física a 1.797 sujetos y se han desarrollado cuatro grupos focales. A partir de la información recabada, el grupo de trabajo ha aprobado llevar a cabo 23 intervenciones, de las cuales están en marcha o ya ejecutadas 19. IkasSasoi, ha permitido conocer en profundidad los hábitos y percepciones de las y los jóvenes y ha generado una red intersectorial consolidada. El programa está ubicando a la población adolescente en la agenda de las políticas locales vinculadas con la actividad física. Los principales aspectos a mejorar se refieren al incumplimiento de los plazos de intervención en acciones concretas y a la desigual implicación de los agentes, especialmente en los centros educativo

4polar3D : Single molecule 3D orientation imaging of dense actin networks using ratiometric polarization splitting

Single Molecule Orientation and Localization Microscopy (SMOLM) aims at simultaneously measuring the position and orientation of single molecules, generating orientation-encoded super-resolved images by estimating both their 3D mean orientation and the extent of their angular fluctuations(wobble). Most existing SMOLM approaches rely on the engineering of single molecules’ point spread functions, which requires complex optical setups and long computational times that can be an obstacle in dense cellular environments with high detection density and challenging imaging conditions. In this work, we propose a simpler and effective method named 4polar3D, based on the estimation of single molecule intensities projected onto four polarized channels with controlled numerical apertures. This strategy enables 3D orientation measurements of single molecules in addition to their 2D localization in a fast processing step. We demonstrate that 4polar3D can resolve nanoscale molecular organization in whole cells’ crowded structures, uncovering 3D-oriented actin filament networks in densely packed lamellipodia and podosomes

First-in-human evaluation of a novel sirolimus-eluting ultra-high molecular weight APTITUDE bioresorbable scaffold: 9- and 24-month imaging and clinical results of the RENASCENT II trial

Aims: The novel sirolimus-eluting ultra-high molecular weight APTITUDE bioreabsorbable vascular scaffold (BRS) displays higher mechanical strength, expansion capabilities and resistance to fracture compared to other BRS technologies. RENASCENT II is a prospective, multicentre first-in-human clinical study evaluating the clinical performance of the APTITUDE BRS in the treatment of single de novo coronary lesions among patients undergoing percutaneous coronary intervention. Methods and results: The APTITUDE BRS was tested in a prospective study in two countries (Italy and Colombia). Study objectives were angiographic in-scaffold late lumen loss (IS-LLL) measured by quantitative coronary angiography (QCA) and target vessel failure (TVF) defined as the composite rate of cardiac death, target vessel myocardial infarction (TV-MI) or ischaemia-driven target lesion revascularisation (TLR) at 9 and 24 months. A total of 60 patients were enrolled. All patients underwent lesion predilatation and 46 patients (76.7%) underwent post-dilatation. Clinical device and procedural success were 98.3% (59/60 patients) and 100%, respectively. Angiographic late lumen loss was 0.19±0.26 mm at 9 months and 0.3±0.41 mm at 24 months. At 9 months, TVF occurred in 2/59 patients (3.4%) due to TV-MI but there was no TLR. No further cases of TVF, MACE or stent thrombosis were reported up to 24-month follow-up. Conclusions: In this multicentre prospective study, the APTITUDE BRS was shown to be safe and effective in the treatment of single coronary lesions at 24-month clinical follow-up