Vaccination Prophylaxis for Bacterial Infections

Introduction: Bacterial infections affect an increasing portion of the population and pose a significant threat to global health. Treatment has become more challenging due to bacterial resistance to various antibiotics, leading to the need for alternative methods to combat these infections. One such method is vaccination prophylaxis.Aim: The aim of this study is to analyze data from scientific publications and assess the effectiveness of vaccines against some of the most common bacterial pathogens.Materials and Methods: This study was conducted using a literature review approach, focusing on articles published in PubMed and Google Scholar from 2019 to 2024.Results: Our study found that vaccination prophylaxis is a widely used method for combating various bacterial pathogens. A strict vaccination program and adherence to it significantly reduce the spread of life-threatening infectious diseases. In Bulgaria, immunization begins in maternity wards, while in some European countries, it starts after the second month of life.Conclusion: The increasing spread of bacterial infections can be mitigated by developing new vaccines and optimizing the prophylaxis of existing ones. Adherence to immunization schedules significantly reduces the spread of serious infections and improves public health

Phytochemical Profile, Antioxidant Potential, Antimicrobial Activity, and Cytotoxicity of Dry Extract from <i>Rosa damascena</i> Mill

Dry rose extract (DRE) obtained industrially by aqueous ethanol extraction from R. damascena flowers and its phenolic-enriched fraction, obtained by re-extraction with ethyl acetate (EAE) were the subject of this study. 1H NMR of DRE allowed the identification and quantitation of fructose and glucose, while the combined use of HPLC-DAD-ESIMS and HPLC-HRMS showed the presence of 14 kaempferol glycosides, 12 quercetin glycosides, 4 phenolic acids and their esters, 4 galloyl glycosides, 7 ellagitannins, and quinic acid. In addition, the structures of 13 of the flavonoid glycosides were further confirmed by NMR. EAE was found to be richer in TPC and TFC and showed better antioxidant activity (DPPH, ABTS, and FRAP) compared to DRE. Both extracts displayed significant activity against Propionibacterium acnes, Staphylococcus aureus, and S. epidermidis, but showed no activity against Candida albicans. Toxicity tests on normal human skin fibroblasts revealed low toxicity for both extracts with stronger effects observed at 24 hours of treatment that were compensated for over the following two days. Human hepatocarcinoma (HepG2) cells exhibited an opposite response after treatment with a concentration above 350 µg/mL for EAE and 500 µg/mL for DRE, showing increased toxicity after the third day of treatment. Lower concentrations were non-toxic and did not significantly affect the cell cycle parameters of either of the cell lines