24 research outputs found
Key parameters in the epidemiological model.
<p>Key parameters in the epidemiological model.</p
Variation in pertussis incidence and costs according to the age at which the adult booster dose is administered (Childhood vaccination+adolescent+cocoon+1 booster dose for adult vaccination - steady-state situation).
<p>Variation in pertussis incidence and costs according to the age at which the adult booster dose is administered (Childhood vaccination+adolescent+cocoon+1 booster dose for adult vaccination - steady-state situation).</p
Average annual predicted costs of pertussis and vaccination from 2006 to 2106.
<p>Average annual predicted costs of pertussis and vaccination from 2006 to 2106.</p
Table1_Novel mutations in the SGCA gene in unrelated Vietnamese patients with limb-girdle muscular dystrophies disease.DOCX
Background: Limb-girdle muscular dystrophy (LGMD) is a group of inherited neuromuscular disorders characterized by atrophy and weakness in the shoulders and hips. Over 30 subtypes have been described in five dominant (LGMD type 1 or LGMDD) and 27 recessive (LGMD type 2 or LGMDR). Each subtype involves a mutation in a single gene and has high heterogeneity in age of onset, expression, progression, and prognosis. In addition, the lack of understanding of the disease and the vague, nonspecific symptoms of LGMD subtypes make diagnosis difficult. Even as next-generation sequencing (NGS) genetic testing has become commonplace, some patients remain undiagnosed for many years.Methods: To identify LGMD-associated mutations, Targeted sequencing was performed in the patients and Sanger sequencing was performed in patients and family members. The in silico analysis tools such as Fathmm, M-CAP, Mutation Taster, PolyPhen 2, PROVEAN, REVEL, SIFT, MaxEntScan, Spliceailookup, Human Splicing Finder, NetGene2, and Fruitfly were used to predict the influence of the novel mutations. The pathogenicity of the mutation was interpreted according to the ACMG guidelines.Results: In this study, six patients from four different Vietnamese families were collected for genetic analysis at The Center for Gene and Protein Research and The Department of Molecular Pathology Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam. Based on clinical symptoms and serum creatine kinase (CK) levels, the patients were diagnosed with limb-girdle muscular dystrophies. Five mutations, including four (c.229C>T, p.Arg77Cys; exon one to three deletion; c.983 + 5G>C; and c.257_258insTGGCT, p.Phe88Leufs*125) in the SGCA gene and one (c.946-4_946-1delACAG) in the CAPN3 gene, were detected in six LGMD patients from four unrelated Vietnamese families. Two homozygous mutations (c.983 + 5G>C and c.257_258insTGGCT) in the SGCA gene were novel. These mutations were identified as the cause of the disease in the patients.Conclusion: Our results contribute to the general understanding of the etiology of the disease and provide the basis for definitive diagnosis and support genetic counseling and prenatal screening.</p
Distribution of outcomes in case of exposure.
<p>Distribution of outcomes in case of contact with an infectious person according to immunological status. Values for susceptible, immune and natural waning are taken from <i>Van Rie et al.</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006284#pone.0006284-Coudeville1" target="_blank">[25]</a>. Values for vaccine-related compartments are estimated using <i>Bisgard et al.</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006284#pone.0006284-Bisgard2" target="_blank">[<i>31</i>]</a> and <i>Ward et al.</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006284#pone.0006284-Ward1" target="_blank">[10]</a>. Figures in parentheses define the range used in the sensitivity analysis.</p
Table2_Novel mutations in the SGCA gene in unrelated Vietnamese patients with limb-girdle muscular dystrophies disease.DOCX
Background: Limb-girdle muscular dystrophy (LGMD) is a group of inherited neuromuscular disorders characterized by atrophy and weakness in the shoulders and hips. Over 30 subtypes have been described in five dominant (LGMD type 1 or LGMDD) and 27 recessive (LGMD type 2 or LGMDR). Each subtype involves a mutation in a single gene and has high heterogeneity in age of onset, expression, progression, and prognosis. In addition, the lack of understanding of the disease and the vague, nonspecific symptoms of LGMD subtypes make diagnosis difficult. Even as next-generation sequencing (NGS) genetic testing has become commonplace, some patients remain undiagnosed for many years.Methods: To identify LGMD-associated mutations, Targeted sequencing was performed in the patients and Sanger sequencing was performed in patients and family members. The in silico analysis tools such as Fathmm, M-CAP, Mutation Taster, PolyPhen 2, PROVEAN, REVEL, SIFT, MaxEntScan, Spliceailookup, Human Splicing Finder, NetGene2, and Fruitfly were used to predict the influence of the novel mutations. The pathogenicity of the mutation was interpreted according to the ACMG guidelines.Results: In this study, six patients from four different Vietnamese families were collected for genetic analysis at The Center for Gene and Protein Research and The Department of Molecular Pathology Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam. Based on clinical symptoms and serum creatine kinase (CK) levels, the patients were diagnosed with limb-girdle muscular dystrophies. Five mutations, including four (c.229C>T, p.Arg77Cys; exon one to three deletion; c.983 + 5G>C; and c.257_258insTGGCT, p.Phe88Leufs*125) in the SGCA gene and one (c.946-4_946-1delACAG) in the CAPN3 gene, were detected in six LGMD patients from four unrelated Vietnamese families. Two homozygous mutations (c.983 + 5G>C and c.257_258insTGGCT) in the SGCA gene were novel. These mutations were identified as the cause of the disease in the patients.Conclusion: Our results contribute to the general understanding of the etiology of the disease and provide the basis for definitive diagnosis and support genetic counseling and prenatal screening.</p
Table3_Novel mutations in the SGCA gene in unrelated Vietnamese patients with limb-girdle muscular dystrophies disease.DOCX
Background: Limb-girdle muscular dystrophy (LGMD) is a group of inherited neuromuscular disorders characterized by atrophy and weakness in the shoulders and hips. Over 30 subtypes have been described in five dominant (LGMD type 1 or LGMDD) and 27 recessive (LGMD type 2 or LGMDR). Each subtype involves a mutation in a single gene and has high heterogeneity in age of onset, expression, progression, and prognosis. In addition, the lack of understanding of the disease and the vague, nonspecific symptoms of LGMD subtypes make diagnosis difficult. Even as next-generation sequencing (NGS) genetic testing has become commonplace, some patients remain undiagnosed for many years.Methods: To identify LGMD-associated mutations, Targeted sequencing was performed in the patients and Sanger sequencing was performed in patients and family members. The in silico analysis tools such as Fathmm, M-CAP, Mutation Taster, PolyPhen 2, PROVEAN, REVEL, SIFT, MaxEntScan, Spliceailookup, Human Splicing Finder, NetGene2, and Fruitfly were used to predict the influence of the novel mutations. The pathogenicity of the mutation was interpreted according to the ACMG guidelines.Results: In this study, six patients from four different Vietnamese families were collected for genetic analysis at The Center for Gene and Protein Research and The Department of Molecular Pathology Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam. Based on clinical symptoms and serum creatine kinase (CK) levels, the patients were diagnosed with limb-girdle muscular dystrophies. Five mutations, including four (c.229C>T, p.Arg77Cys; exon one to three deletion; c.983 + 5G>C; and c.257_258insTGGCT, p.Phe88Leufs*125) in the SGCA gene and one (c.946-4_946-1delACAG) in the CAPN3 gene, were detected in six LGMD patients from four unrelated Vietnamese families. Two homozygous mutations (c.983 + 5G>C and c.257_258insTGGCT) in the SGCA gene were novel. These mutations were identified as the cause of the disease in the patients.Conclusion: Our results contribute to the general understanding of the etiology of the disease and provide the basis for definitive diagnosis and support genetic counseling and prenatal screening.</p
Estimates for the short term cost per case of pertussis infection (ranges used in sensitivity analyzes presented in parentheses).
*<p>Applies only to the fraction of patients with long term sequelae following infection.</p>**<p>Applies only to the fraction of patients with fatal cases of pertussis or long term sequelae following infection.</p>+<p>Caro et al. [43] updated to 2006 using CPI for medical care (<a href="http://www.bls.gov" target="_blank">www.bls.gov</a>).</p>#<p>Lee et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006284#pone.0006284-Lee1" target="_blank">[20]</a> updated to 2006 using CPI for medical care (<a href="http://www.bls.gov" target="_blank">www.bls.gov</a>).</p
Two new linear acetogenins from the fruits of <i>Goniothalamus gracilipes</i>
<p>Two new linear acetogenins, gracilipin A (<b>1</b>) and methylsaccopetrin A (<b>2</b>) along with seven known compounds, saccopetrin A (<b>3</b>), 7,3′,4′-trimethylquercetin (<b>4</b>), rhamnazin (<b>5</b>), casticin (<b>6</b>), isokanugin (<b>7</b>), melisimplexin (<b>8</b>) and 5-hydroxy-3,7-dimethoxy-3′,4′-methylenedioxyflavone (<b>9</b>) were isolated from the fruits of <i>Goniothalamus gracilipes</i> Bân. Their structures were established by spectral analysis, such as mass spectrometry, 1D-NMR, 2D-NMR and circular dichroism (CD). Compounds <b>1</b> and <b>3</b> showed cytotoxic activity against KB cell line with IC<sub>50</sub> values of 14.6 and 15.3 μM, respectively.</p
Prostratin and 12‑<i>O</i>‑Tetradecanoylphorbol 13-Acetate Are Potent and Selective Inhibitors of Chikungunya Virus Replication
A chemical study of the Vietnamese plant species <i>Trigonostemon
howii</i> led to the isolation of a new tigliane-type diterpenoid,
trigowiin A (<b>1</b>), along with several known coumarins and
phenylpropanoids. The planar structure and the relative configuration
of compound <b>1</b> were elucidated based on spectroscopic
analysis, including 1D- and 2D-NMR experiments, mass spectrometry,
and comparison with literature data. Trigowiin A (<b>1</b>)
exhibited moderate antiviral activity in a virus-cell-based assay
for Chikungunya virus (CHIKV). Since the structure of compound <b>1</b> is closely related to those of well-known tigliane diterpenoids
such as prostratin (<b>2</b>), phorbol (<b>3</b>), 12-<i>O</i>-tetradecanoylphorbol 13-acetate (TPA) (<b>4</b>),
and 4α-TPA (<b>5</b>), the antiviral activity of the latter
compounds was also evaluated against CHIKV, as well as in virus-cell-based
assays of two additional members of the genus <i>Alphavirus</i> (Sindbis virus, SINV, and Semliki forest virus, SFV). Whereas prostratin
inhibited CHIKV replication with a moderate EC<sub>50</sub> of 2.6
μM and a selectivity index (SI) approximating 30, compound <b>4</b> proved to be an extremely potent inhibitor, with an EC<sub>50</sub> of ∼3 nM and a SI near 2000. Interestingly, no or
very little activity was observed on the replication of SINV and SFV