AMOVA analysis of differences in diversity of endophytic bacteria in different host plant species.

AMOVA analysis of differences in diversity of endophytic bacteria in different host plant species.</p

Variation of relative abundances of dominant bacterial groups with the host plant species and sampling months.

<p>Relative abundances of the dominant phyla in the leaf endophytic bacterial communities from different host plants (A); Relative abundances of the dominant bacterial genera across different host plant species (B) and across different sampling months (C).</p

AMOVA analysis of differences in diversity of endophytic bacteria in different.

<p>sampling months.</p

LEfSe analysis identification of biomarker microbes for endophytic bacterial communities for different host plant species.

<p>LEfSe analysis identification of biomarker microbes for endophytic bacterial communities for different host plant species.</p

LEfSe analysis identification of biomarker microbes for endophytic bacterial communities for different months of sample collection.

<p>LEfSe analysis identification of biomarker microbes for endophytic bacterial communities for different months of sample collection.</p

Standard curves and graphs generated using 10-fold diluted genomic DNA and genomic DNA mixed with orange rind extract or insect crude DNA.

<p>A1/A2, A3/A4 & A5/A6: Graphs/standard curve generated using primer and probe set Xf.CVC.fim1 with genomic DNA, genomic DNA mixed with orange rind extract and genomic DNA mixed with insect crude DNA, respectively; B1/B2, B3/B4 & B5/B6: Graphs/standard curve generated using primer/ probe set Xf.CVC.pib4 with genomic DNA, genomic DNA mixed with orange rind extract and genomic DNA mixed with insect crude DNA, respectively; C1/C2, C3/C4 & C5/C6: Graphs/standard curve generated using primer/ probe set Xf.csp6 with genomic DNA, genomic DNA mixed with orange rind extract and genomic DNA mixed with insect crude DNA, respectively.</p

Razor Ex BioDetection system graph obtained after amplification of plasmid DNA (carrying the target gene sequence of corresponding primer set) and <i>Xylella fastidiosa</i> subsp. <i>pauca</i> genomic DNA (CVC50031) using primer and probe sets Xf.CVC.fim1, Xf.CVC.pib4 and Xf.csp6.

<p>A1, B1 and C1 are positive controls with estimated Ct values of 23, 26 and 20 for primer/probe sets Xf.CVC.fim1, Xf.CVC.pib4 and Xf.csp6, respectively. <i>X. fastidiosa</i> genomic DNA tested in two replicates with primer/probe sets Xf.CVC.fim1 (A2 & A3), Xf.CVC.pib4 (B2 & B3) and Xf.csp6 (C2 & C3) with estimated Ct value of 24, 24 and 20, respectively. N shows the non-template controls (water) for each primer/probe set. </p

Standard graph showing 10-fold serial dilutions of plasmid DNA (carrying the target gene sequence of corresponding primer set) using primer and probe sets.

<p>Xf.CVC.fim1 (A), Xf.CVC.pib4 (B), and Xf.csp6 (C). R2 = linear correlation; Ex = reaction efficiency; Y = slope.</p

Graph from the Razor Ex BioDetection system after amplification of plasmid DNA (carrying the target gene sequence of corresponding primer set) and <i>Xylella fastidiosa</i> infected grape and oak plant samples (two samples for each plant), using the primer and probe set Xf.csp6.

<p>A: positive controls; estimated Ct value 24; B: <i>X. fastidiosa</i> infected grape (sample G1 and G2) and oak (sample O1 and O2) genomic DNA; estimated Ct values 33-35; N: non-template control (water). Each reaction was performed in two replicates. </p

Bar code generated to upload the fast PCR cycling program for detection of <i>Xylella fastidiosa</i> and <i>Xylella fastidiosa</i> subsp. <i>pauca</i> in Razor Ex BioDetection system.

<p>Bar code generated to upload the fast PCR cycling program for detection of <i>Xylella fastidiosa</i> and <i>Xylella fastidiosa</i> subsp. <i>pauca</i> in Razor Ex BioDetection system.</p