Table of purification of lectin from nagaimo tubers (100 grams).

<p>Table of purification of lectin from nagaimo tubers (100 grams).</p

Profile of elution in purification of nagaimo lectin.

<p>Elution profile of (A) crude nagaimo tuber extract from Q-Sepharose, (B) Fraction II from Mono Q, and (C) Fraction V from Superdex 75. The shaded regions represent the fractions with hemagglutinating activity that were collected in each step.</p

Results of SDS-APGE.

<p>SDS-PAGE of (A) crude nagaimo tuber extract, (B) Fraction II from Q-Sepharose, (C) Fraction V from Mono Q, (D) Purified nagaimo lectin from Superdex 75, and (E) Purified nagaimo lectin without addition of the reducing agent, β-mercaptoethanol. Lane 1 of each panel: Molecular weight markers (GE Healthcare) including phosphorylase b (97 kDa), bovine serum albumin (66 kDa), ovalbumin (45 kDa), carbonic anhydrase (30 kDa), soybean trypsin inhibitor (20 kDa) and α-lactalbumin (14.4 kDa). Lane 2 of each panel: nagaimo protein samples.</p

Results of MTT assay of (A) MCF7 cells and (B) HepG2 cells treated with nagaimo lectin, with or without concurrent administration of 100 mM galactose.

<p>The presence of 100 mM galactose could not curtail the retarding effect of nagaimo lectin on the growth of MCF7 and HepG2 cells. Results represent mean±SD (n = 3).</p

Flow cytometry analysis on JC-1 staining.

<p>Flow cytometry analysis of MCF7 cells after treatment with nagaimo lectin (A) with or (B) without the presence of 100 mM nagaimo lectin for 24 hours, followed by staining with JC-1.</p

Results of MTT assay on different cell lines.

<p>Results of MTT assay of MCF7, HepG2 and CNE2 cells treated with different concentrations of nagaimo lectin. Results represent mean±SD (n = 3).</p

N-terminal amino acid sequence of nagaimo lectin, and the amino acid sequences of other proteins with highest homology based on the GenBank Database.

<p>The amino acids in other proteins differ from that of nagaimo lectin are underlined.</p

Results of MTT assay of MCF7 cells treated with nagaimo lectin that were pre-treated at different (A) temperatures and (B) pH values for 30 minutes.

<p>The presence of 100 mM galactose could not curtail the retarding effect of Nagaimo lectin on the growth of MCF7 and HepG2 cells. Results represent mean±SD (n = 3).</p

Flow cytometry analysis on Annexin V-FITC and PI staining.

<p>Flow cytometry analysis of MCF7 cells after treatment with nagaimo lectin (A) with or (B) without the presence of 100 mM nagaimo lectin for 24 hours, followed by staining with Annexin V-FITC.</p

Effect of (A) different temperatures, (B) different pH, and (C) specific carbohydrates on hemagglutinating activity of Nagaimo lectin.

<p>Effect of (A) different temperatures, (B) different pH, and (C) specific carbohydrates on hemagglutinating activity of Nagaimo lectin.</p