Practical Synthetic Route to Functionalized Rhodamine Dyes

An efficient method for the synthesis of functionalized rhodamine derivatives has been developed. Multigram quantities of these water-soluble fluorophores can be prepared from inexpensive precursors and purified without the use of chromatography. A series of protein-reactive functional groups has been installed through subsequent reactions, providing materials for biomolecule modification. For multicolor applications, a solid-phase purification strategy has been developed to afford rhodamine derivatives possessing a wide range of spectral properties

An Affinity-Based Method for the Purification of Fluorescently-Labeled Biomolecules

Due to the difficulty of separating mixtures of labeled and unlabeled biomolecules, a general new method for the affinity purification of modified proteins has been developed. A Sepharose-based solid support bearing β-cyclodextrin groups was used to capture chromophore-modified proteins selectively, while unmodified proteins remained in solution. After isolation of the resin, the modified proteins were released by treating the sample with a competitive cyclodextrin binder, such as adamantane carboxylic acid. This procedure was demonstrated for several dyes displaying a wide range of spectral characteristics and diverse chemical structures. Preliminary studies have shown that this method can also be used to enrich modified peptide fragments present in proteolytic digests. This technique is anticipated to accelerate the development of new protein modification reactions and could provide a useful tool for proteomics applications

Additional file 1: of Low concordance of oral and genital HPV infection among male patients with sexually transmitted infections in Vietnam

Table S1. HPV genotypes in different samples in each HPV DNA positive patients. Table S2. Risk factors for overall HPV infection. (DOCX 25 kb

sj-docx-1-tpx-10.1177_01926233231224468 – Supplemental material for Assessment of Color Reproducibility and Mitigation of Color Variation in Whole Slide Image Scanners for Toxicologic Pathology

Supplemental material, sj-docx-1-tpx-10.1177_01926233231224468 for Assessment of Color Reproducibility and Mitigation of Color Variation in Whole Slide Image Scanners for Toxicologic Pathology by Mei-Lan Chu, Xing-Yue M. Ge, Jeffrey Eastham, Trung Nguyen, Reina N. Fuji, Ruth Sullivan and Daniel Ruderman in Toxicologic Pathology</p

Supplementary document for Multiplexed Imaging in Live Cells Using Pulsed Interleaved Excitation Spectral FLIM - 6805766.pdf

Supplemental Notes, Figures, Table

Biocompatible Polymeric Nanoparticles Degrade and Release Cargo in Response to Biologically Relevant Levels of Hydrogen Peroxide

Oxidative stress is caused predominantly by accumulation of hydrogen peroxide and distinguishes inflamed tissue from healthy tissue. Hydrogen peroxide could potentially be useful as a stimulus for targeted drug delivery to diseased tissue. However, current polymeric systems are not sensitive to biologically relevant concentrations of H₂O₂ (50–100 μM). Here we report a new biocompatible polymeric capsule capable of undergoing backbone degradation and thus release upon exposure to such concentrations of hydrogen peroxide. Two polymeric structures were developed differing with respect to the linkage between the boronic ester group and the polymeric backbone: either direct (<b>1</b>) or via an ether linkage (<b>2</b>). Both polymers are stable in aqueous solution at normal pH, and exposure to peroxide induces the removal of the boronic ester protecting groups at physiological pH and temperature, revealing phenols along the backbone, which undergo quinone methide rearrangement to lead to polymer degradation. Considerably faster backbone degradation was observed for polymer <b>2</b> over polymer <b>1</b> by NMR and GPC. Nanoparticles were formulated from these novel materials to analyze their oxidation triggered release properties. While nanoparticles formulated from polymer <b>1</b> only released 50% of the reporter dye after exposure to 1 mM H₂O₂ for 26 h, nanoparticles formulated from polymer <b>2</b> did so within 10 h and were able to release their cargo selectively in biologically relevant concentrations of H₂O₂. Nanoparticles formulated from polymer <b>2</b> showed a 2-fold enhancement of release upon incubation with activated neutrophils, while controls showed a nonspecific response to ROS producing cells. These polymers represent a novel, biologically relevant, and biocompatible approach to biodegradable H₂O₂-triggered release systems that can degrade into small molecules, release their cargo, and should be easily cleared by the body

Mass Transport Investigation for High-performance Ammonia Electrosynthesis based on Phosphonium Proton Shuttles

Mass Transport Investigation for High-performance Ammonia Electrosynthesis based on Phosphonium Proton Shuttle

Supplementary document for Two-photon autofluorescence lifetime assay of rabbit photoreceptors and retinal pigment epithelium during light-dark visual cycles in rabbit retina - 6923105.pdf

Supplemental Informatio

Supplementary document for Two-photon autofluorescence lifetime assay of rabbit photoreceptors and retinal pigment epithelium during light-dark visual cycles in rabbit retina - 6878395.pdf

Supplemental Documen

Selected patient characteristics when confirmed and clinically suspected diagnoses are combined.

<p>All continuous data are median (range).</p>*<p>AEM vs. TBM & AEM vs. BM, ps≤0.000.</p>†<p>BM vs. TBM & BM vs. AEM, ps = 0.000.</p>#<p>BM vs. TBM p = 0.002, BM vs. AEM, p = 0.000.</p>‡<p>AEM vs. BM & TBM, ps 0.001.</p>§<p>AEM vs. BM & TBM, ps<0.000, TBM vs. AEM, p = 0.018.</p