15 research outputs found

    Methods of the development strategy of service companies: Logistical approach

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    The urgency of the analyzed issue is due to lack of attention of heads of service companies to the theory and methodology of strategic management, methods and models of management decision-making in times of economic instability. The purpose of the article is to develop theoretical positions and methodical recommendations on the formation of the logistical approach to the development strategy of service companies. The leading approach to the study of this issue is the logistical approach, which allows identifying the most significant factors, carrying out the quantitative assessment of their interaction with each other and determining the extent of their influence on the parameters of the system under research. This research presents a methodology for the selection of optimal functional business strategies of service companies from the alternative, based on the use of economic and mathematical modeling techniques. The authors assess the parameters of the micro, macro and internal environment of the company, represent the company’s business profile, a general development strategy of based on the determination of optimal logistics, marketing, production, financial and human resource management strategies for individual strategic business areas. The contents of the article may be useful for managers of service companies, auto-transport enterprises in making rational decisions on the formation of the optimal business development strategy in uncertain environmental conditions. © 2016 Toymentseva et al

    The use of logistics n the quality parameters control system of material flow

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    The relevance of the research problem is conditioned on the need to justify the use of the logistics methodologies in the quality parameters control process of material flows. The goal of the article is to develop theoretical principles and practical recommendations for logistical system control in material flows quality parameters. A leading approach to study this problem is a set of scientific knowledge and special methods, allowing identifying the main trends and features for the quality parameters control of material flows. The main results of the research with the scientific novelty are the following: - grounded conceptual framework for the qualitative parameters management of material flows and business processes; - defined the relationship of the evaluation criteria to characterize products and processes, and to highlight typical and assigned material flow characteristics; - proved the necessity of quality parameters logistical system control of material flows. The article can be useful to set up the quality parameters control system of material flow in the micro – mesa-logistics systems to optimize total costs, improve quality, material flow and processes and, as a consequence, improve the products competitiveness. © 2016 Karpova et al

    The Genetic Mechanism of Resistance to Antibiotics in Bacillus pumilus 3-19 Strain

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    © 2016, Springer Science+Business Media New York.Treatment of bacterial infections becomes increasingly complicated due to the emergence of bacterial resistance to antimicrobial agents. Until now, most studies on bacterial antibiotic resistance have focused mainly on clinically relevant isolates pathogenic microorganisms and lactic acid bacteria. Very limited information on the antimicrobial susceptibility profiles of Bacillus spp. is available. In this paper, we used Bacillus pumilus 3-19 strain, a derivative of a wild B. pumilus 7P strain that has acquired resistance to streptomycin. Comparative analysis of genomes showed that B. pumilus 3-19 became resistant to streptomycine due to a mutation in 56 codon of the rpsL gene (S12 protein of 30S ribosomal subunit) that resulted in the replacement of lysine with asparagine in the binding site of streptomycin. Bioinformatic analysis of rpoB gene (β-subunit of RNA polymerase) showed that there is also a point mutation in 185 codon, which can lead to rifampicin resistance. Nevertheless, B. pumilus 3-19 strain remained sensitive to rifampicin in disc diffusion assay

    Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage

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    © 2016 Pleiades Publishing, Ltd. Using the expression vector pGP382 containing a constitutive promoter (P degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer's disease are presented

    The Genetic Mechanism of Resistance to Antibiotics in Bacillus pumilus 3-19 Strain

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    © 2016, Springer Science+Business Media New York.Treatment of bacterial infections becomes increasingly complicated due to the emergence of bacterial resistance to antimicrobial agents. Until now, most studies on bacterial antibiotic resistance have focused mainly on clinically relevant isolates pathogenic microorganisms and lactic acid bacteria. Very limited information on the antimicrobial susceptibility profiles of Bacillus spp. is available. In this paper, we used Bacillus pumilus 3-19 strain, a derivative of a wild B. pumilus 7P strain that has acquired resistance to streptomycin. Comparative analysis of genomes showed that B. pumilus 3-19 became resistant to streptomycine due to a mutation in 56 codon of the rpsL gene (S12 protein of 30S ribosomal subunit) that resulted in the replacement of lysine with asparagine in the binding site of streptomycin. Bioinformatic analysis of rpoB gene (β-subunit of RNA polymerase) showed that there is also a point mutation in 185 codon, which can lead to rifampicin resistance. Nevertheless, B. pumilus 3-19 strain remained sensitive to rifampicin in disc diffusion assay

    The Genetic Mechanism of Resistance to Antibiotics in Bacillus pumilus 3-19 Strain

    Get PDF
    © 2016, Springer Science+Business Media New York.Treatment of bacterial infections becomes increasingly complicated due to the emergence of bacterial resistance to antimicrobial agents. Until now, most studies on bacterial antibiotic resistance have focused mainly on clinically relevant isolates pathogenic microorganisms and lactic acid bacteria. Very limited information on the antimicrobial susceptibility profiles of Bacillus spp. is available. In this paper, we used Bacillus pumilus 3-19 strain, a derivative of a wild B. pumilus 7P strain that has acquired resistance to streptomycin. Comparative analysis of genomes showed that B. pumilus 3-19 became resistant to streptomycine due to a mutation in 56 codon of the rpsL gene (S12 protein of 30S ribosomal subunit) that resulted in the replacement of lysine with asparagine in the binding site of streptomycin. Bioinformatic analysis of rpoB gene (β-subunit of RNA polymerase) showed that there is also a point mutation in 185 codon, which can lead to rifampicin resistance. Nevertheless, B. pumilus 3-19 strain remained sensitive to rifampicin in disc diffusion assay

    Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage

    No full text
    © 2016 Pleiades Publishing, Ltd. Using the expression vector pGP382 containing a constitutive promoter (P degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer's disease are presented

    The Genetic Mechanism of Resistance to Antibiotics in Bacillus pumilus 3-19 Strain

    No full text
    © 2016, Springer Science+Business Media New York.Treatment of bacterial infections becomes increasingly complicated due to the emergence of bacterial resistance to antimicrobial agents. Until now, most studies on bacterial antibiotic resistance have focused mainly on clinically relevant isolates pathogenic microorganisms and lactic acid bacteria. Very limited information on the antimicrobial susceptibility profiles of Bacillus spp. is available. In this paper, we used Bacillus pumilus 3-19 strain, a derivative of a wild B. pumilus 7P strain that has acquired resistance to streptomycin. Comparative analysis of genomes showed that B. pumilus 3-19 became resistant to streptomycine due to a mutation in 56 codon of the rpsL gene (S12 protein of 30S ribosomal subunit) that resulted in the replacement of lysine with asparagine in the binding site of streptomycin. Bioinformatic analysis of rpoB gene (β-subunit of RNA polymerase) showed that there is also a point mutation in 185 codon, which can lead to rifampicin resistance. Nevertheless, B. pumilus 3-19 strain remained sensitive to rifampicin in disc diffusion assay

    Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage

    Get PDF
    © 2016 Pleiades Publishing, Ltd. Using the expression vector pGP382 containing a constitutive promoter (P degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer's disease are presented

    Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage

    No full text
    © 2016 Pleiades Publishing, Ltd. Using the expression vector pGP382 containing a constitutive promoter (P degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer's disease are presented
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