sj-docx-3-tct-10.1177_15330338231164359 - Supplemental material for LINC00460 Facilitates Cell Proliferation and Inhibits Ferroptosis in Breast Cancer Through the miR-320a/MAL2 Axis

Supplemental material, sj-docx-3-tct-10.1177_15330338231164359 for LINC00460 Facilitates Cell Proliferation and Inhibits Ferroptosis in Breast Cancer Through the miR-320a/MAL2 Axis by Chuanqiang Zhang, Liang Xu, Xiaowei Li, Yueqiu Chen, Tongguo Shi and Qiang Wang in Technology in Cancer Research & Treatment</p

sj-docx-1-tct-10.1177_15330338231164359 - Supplemental material for LINC00460 Facilitates Cell Proliferation and Inhibits Ferroptosis in Breast Cancer Through the miR-320a/MAL2 Axis

Supplemental material, sj-docx-1-tct-10.1177_15330338231164359 for LINC00460 Facilitates Cell Proliferation and Inhibits Ferroptosis in Breast Cancer Through the miR-320a/MAL2 Axis by Chuanqiang Zhang, Liang Xu, Xiaowei Li, Yueqiu Chen, Tongguo Shi and Qiang Wang in Technology in Cancer Research & Treatment</p

Additional file 1 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 1: Figure S1. Correlation analysis of the expression of PKCs and B7-H4 in CRC based on the LinkedOmics and GEPIA databases. A Correlation analysis between PKCs and B7-H4; B comparison of PKRCA and PRKCD expression in cancer and adjacent tissues

Additional file 3 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 3: Figure S3. PKCδ mediated B7-H4 upregulation in CRC cell lines. HCT116 and SW620 cells were treated with various concentrations of TPA (A) or rottlerin (B) for 20 h. C The HCT116 and SW620 cell lines were treated with 1 μM rottlerin and 100 nM TPA for 24 h, and B7-H4 levels were determined by Western blotting

Additional file 7 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 7: Table S1. The primers of real-time PCR and the siRNAs

Additional file 2 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 2: Figure S2. Western blot analysis was performed to detect the expression of B7-H4 in CRC cell lines. The protein levels of B7-H4 and p-PKCδ in the NCM460, SW480, HCT116, SW620 and RKO cell lines were determined

Additional file 5 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 5: Figure S5. PKCδ inhibited B7-H4 expression via STAT3 in CRC cell lines. Treatment with a PKCδ-specific siRNA reduced the expression of both B7-H4 and STAT3 in HCT116 and SW620 cells (A). HCT116 and SW620 cells were treated with various concentrations of the STAT3 inhibitor cryptotanshinone (B) for 24 h. The cells were harvested to generate whole-cell lysates for detection of the indicated proteins by Western blot analysis

Additional file 6 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 6: Figure S6. The PKCδ/B7-H4 axis promoted the migration of SW620 cells. HCT116 and SW620 cells were treated with a PKCδ-specific siRNA and/or a B7-H4-specific siRNA for 45 h, and B7-H4 protein levels were then determined by Western blot analysis (A). A Transwell assay was performed to examine the constitutive invasion of B7-H4 siRNA/SW620, PKCδ siRNA/SW620, PKCδ siRNA + B7-H4 siRNA/SW620 and con siRNA/SW620 cells (B). A wound healing assay was performed to evaluate the effects of PKCδ and B7-H4 on cell migration (C). The viability of HCT116 cells in different groups was assessed by a CCK-8 assay (D). Experiments were performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001

Additional file 4 of B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer

Additional file 4: Figure S4. PKCδ knockdown inhibited the expression of B7-H4 in CRC cell lines. HCT116 and SW620 cells were treated with a PKCδ-specific siRNA for 45 h. B7-H4 and PKCδ levels were determined by Western blotting (A). HCT116 and SW620 cells were treated with a PKCδ-specific siRNA for 24 h and were then incubated with TPA (100 nM) for 20 h (B, C). The cells were harvested to generate whole-cell lysates for detection of the indicated proteins by Western blot analysis

Additional file 1 of MiR-200/183 family-mediated module biomarker for gastric cancer progression: an AI-assisted bioinformatics method with experimental functional survey

Additional file 1: Figure S1. Expression of RNAs with high RNs scores in GC cell lines. Figure S2. Correlation plot of expression of genes in the module in TCGA dataset. Each cell contains the corresponding correlation coefficient and p-value, and its color indicates correlation according to the color key. Figure S3. Kaplan–Meier survival curve of patients in high-risk and low-risk groups in test cohort and whole cohort of TCGA. Figure S4. Correlation plot of expression of genes in the module in our newly collected clinical samples. Each cell contains the corresponding correlation coefficient and p-value, and its color indicates correlation according to the color key. Table S1. Demographic information for patients with GC in TCGA dataset and validation cohort. Table S2. Primer sequences used for qRT-PCR. Table S3. Sequences of miRNA mimics. Table S4. Parameters for cox regression model