Comparison of experimental and <i>in silico</i> growth rates and growth yields including experimental standard deviations.

<p>Comparison of experimental and <i>in silico</i> growth rates and growth yields including experimental standard deviations.</p

Biosynthetic <i>O-</i>Methylation Protects Cladoniamides from Self-destruction

Bisindole cladoniamides, nanomolar inhibitors of colon cancer cell line HCT-116, contain a rare, indolotryptoline substructure. In this report, the structures of xenocladoniamides A–E (9–13) are described. Compounds 9–13 are generated from a cladoniamide heterologous production system where O-methyltransferase gene claM3 has been inactivated. The results suggest that O-methylation, installed by enzyme ClaM3, is critical to maintaining the structural integrity of the indolotryptoline scaffold. Xenocladoniamides D and E are modestly cytotoxic against colon cancer cell line HCT-116

Comparison of <i>in silico</i> predictions to experimental results for 62 individual nitrogen sources.

<p>Comparison of <i>in silico</i> predictions to experimental results for 62 individual nitrogen sources.</p

Comparison of <i>in silico</i> predictions to experimental results for 22 individual phosphorus sources.

<p>Comparison of <i>in silico</i> predictions to experimental results for 22 individual phosphorus sources.</p

Comparison of nitrogen, phosphorus, and sulfur utilization agreement between this study and previous studies.

<p>The nutrient utilization agreements of the six genome-scale metabolic models created in this study (dashed box) are compared to the same agreements in six previously created models for nitrogen (red), and four for phosphorus (green) and sulfur (purple).</p

Comparison of <i>in silico</i> predictions to experimental results for 39 individual carbon sources.

<p>The 39 carbon sources displayed are those with at least one disagreement.</p

Essential reaction summary for the simulation of conditions representing glucose minimal media, queso fresco, chicken breast, smoked salmon, cantaloupe, and romaine lettuce.

Essential reaction summary for the simulation of conditions representing glucose minimal media, queso fresco, chicken breast, smoked salmon, cantaloupe, and romaine lettuce.</p

Experimentally determined conversion factors.

<p>Experimentally determined conversion factors.</p

Unique nutrient sources metabolized by a single <i>L</i>. <i>monocytogenes</i> strain or serovar.

<p>Unique nutrient sources metabolized by a single <i>L</i>. <i>monocytogenes</i> strain or serovar.</p

Comparison of <i>in silico</i> predictions to experimental results for 11 sulfur sources.

<p>Comparison of <i>in silico</i> predictions to experimental results for 11 sulfur sources.</p