38 research outputs found

    Table_1_Association between systemic immune-inflammation index and post-stroke depression: a cross-sectional study of the national health and nutrition examination survey 2005–2020.XLS

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    BackgroundLess research has linked the Systemic Immune Inflammatory Index (SII) with post-stroke depression (PSD). This study aims to look at any potential connections between SII and PSD.MethodsThe National Health and Nutrition Examination Survey (NHANES), conducted in a population that embodied complete SII and stroke data from 2005 to 2020, was used to perform the current cross-sectional survey. A fitted smoothed curve was used to depict the nonlinear link between SII and PSD, and multiple linear regression analysis demonstrated a positive correlation between SII and PSD.ResultsMultiple linear regression analysis showed that SII and PSD were markedly related [1.11(1.05, 1.17)]. Interaction tests showed that the association between SII and PSD was not statistically different between strata, and age, sex, BMI, income poverty ratio, education level, smoking status, diabetes mellitus, coronary heart disease, and heart failure did not have a significant effect on this positive association (p > 0.05 for interaction). In addition, a nonlinear association between SII and PSD was found using a two-stage linear regression model.ConclusionThe results of our research support the existence of a significant positive correlation between SII levels and PSD. Further prospective trials are required to comprehend SII, which is for the PSD thoroughly.</p

    sj-tif-3-onc-10.1177_11795549221127161 – Supplemental material for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery

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    Supplemental material, sj-tif-3-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology</p

    High glucose-induced upregulation of melatonin receptors in Müller cells.

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    <p>(A) Immunostaining of MT1 and MT2 in cultured primary rat Müller cells. Nuclei were counterstained with SYTO green, Scale bar = 20 µm. (B) High glucose-induced mRNA expression of both melatonin receptors. Cells were treated with 30 mM glucose for 24 and 48 hr. MT1/MT2 expression was measured by Real-time RT-PCR and normalized to β-actin mRNA. CTL: untreated control; Mann: 24.5 mM mannitol as osmolarity control. (C) Western blot of MT1/MT2 proteins in Müller cells treated with high glucose. (D) Quantification data from the Western blots after normalizing to β-actin. Data presented are the average from 3 to 5 separate experiments (mean ± SD). *P<0.05, **P<0.01, as determined by either one-way ANOVA (B) or Student’s t-test (D).</p

    Single Electron Transfer Reductive Deuteration of Acyl Chlorides for the Synthesis of Deuterated Alcohols with a High Deuterium Atom Economy

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    We present a highly deuterium atom economical approach for the synthesis of deuterated alcohols via the single electron transfer (SET) reductive deuteration of acyl chlorides. Cost-effective sodium dispersion and EtOD-d1 were used as the single electron donor and deuterium donor, respectively. Our approach achieved up to 49% deuterium atom economy, which represents the highest deuterium atom economy yet achieved in SET reductive deuteration reactions. With all 20 tested substrates, excellent regioselectivity and >92% deuterium incorporations were obtained. Furthermore, we demonstrated the potential of this methodology by synthesizing four deuterated analogues of pesticides

    Sulfonated Poly(ether ether ketone)/Polypyrrole Core–Shell Nanofibers: A Novel Polymeric Adsorbent/Conducting Polymer Nanostructures for Ultrasensitive Gas Sensors

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    Conducting polymers-based gas sensors have attracted increasing research attention these years. The introduction of inorganic sensitizers (noble metals or inorganic semiconductors) within the conducting polymers-based gas sensors has been regarded as the generally effective route for further enhanced sensors. Here we demonstrate a novel route for highly-efficient conducting polymers-based gas sensors by introduction of polymeric sensitizers (polymeric adsorbent) within the conducting polymeric nanostructures to form one-dimensional polymeric adsorbent/conducting polymer core–shell nanocomposites, via electrospinning and solution-phase polymerization. The adsorption effect of the SPEEK toward NH<sub>3</sub> can facilitate the mass diffusion of NH<sub>3</sub> through the PPy layers, resulting in the enhanced sensing signals. On the basis of the SPEEK/PPy nanofibers, the sensors exhibit large gas responses, even when exposed to very low concentration of NH<sub>3</sub> (20 ppb) at room temperature

    Melatonin-mediated inhibition of VEGF production from Müller cells.

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    <p>(A) Cells were treated with either 30 mM glucose (HG) or glucose and 100 µM melatonin (HG+Mel) for indicated time. The amount of VEGF secreted in the conditioned media was measured by ELISA. (B) Dose-dependent inhibition of VEGF production by melatonin. VEGF production was measured at 48 hr after high glucose stimulation in the presence of different concentrations of melatonin. Data presented are the average of 2 separate experiments. *P<0.05 one-way ANOVA and Tukey’s post-hoc test.</p

    Effects of melatonin on Nrf2 expression and localization in Müller cells.

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    <p>(A) The mRNA level of Nrf2 was measured by real-time RT-PCR after 24 and 48 hr exposure to high glucose with or without melatonin. (B) Immunostaining of Nrf2 localization after high glucose and melatonin treatment. The nuclei were counterstained with DAPI. Scale bar = 5 µm. (C) Western blot analyses of Nrf2 protein after 24 and 48 hr treatment. (D) Effects of luzindole on Nrf2. Data presented are representative of three independent experiments. (*P<0.05).</p

    Protection against high glucose-induced oxidative stress by melatonin.

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    <p>(A) Measurement of cellular glutathione contents in Müller cells exposed to high glucose. Cells were treated with 30 mM glucose (HG) alone or in together with 0.1 mM melatonin (Mel), 50 µM luzindole (Luz) or 50 µM LY294002 (Ly2). Glutathione was measured by the DTNB method. (B) Upregulation of GCLc and GCLm measured by real-time RT-PCR and normalized to β-actin mRNA. (C) Western blot analyses of HO-1 expression after co-treatment with high glucose and indicated concentrations of melatonin (Mel). (D) Effects of luzindole on melatonin-induced upregulation of HO-1. Data presented are average from 3 to 5 experiments (*P<0.05; **P<0.01).</p

    Table_1_Regulation of quorum sensing activities by the stringent response gene rsh in sphingomonads is species-specific and culture condition dependent.DOCX

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    Stringent response and quorum sensing (QS) are two essential mechanisms that control bacterial global metabolism for better survival. Sphingomonads are a clade of bacteria that survive successfully in diverse ecosystems. In silico survey indicated that 36 out of 79 investigated sphingomonads strains contained more than one luxI homolog, the gene responsible for the biosynthesis of QS signal acyl homoserine lactones (AHLs). Investigation of the regulatory effects of the stringent response gene rsh on QS related bioactivities were carried out using rsh mutants of Sphingobium japonicum UT26 and Sphingobium sp. SYK-6, both had three luxI homologs. Results indicated that deletion of rsh upregulated the overall production of AHLs and extracellular polymeric substances (EPS) in both UT26 and SYK-6 in rich medium, but affected expressions of these luxI/luxR homologs in different ways. In the poor medium (1% LB), rsh mutant of SYK-6 significantly lost AHLs production in broth cultivation but not in biofilm cultivation. The regulatory effects of rsh on QS activities were growth phase dependent in UT26 and culture condition dependent in SYK-6. Our results demonstrated the negative regulatory effect of rsh on QS activities in sphingomonads, which were very different from the positive effect found in sphingomonads containing only one luxI/R circuit. This study extends the current knowledge on the intricate networks between stringent response and QS system in sphingomonads, which would help to understand their survival advantage.</p
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