Table_1_Lactobacillus plantarum MH-301 as an effective adjuvant to isotretinoin in the treatment of acne vulgaris: a randomized and open-label trail.DOCX

IntroductionAcne vulgaris is a common chronic inflammatory skin disease originating in the sebaceous gland units of the skin follicles. Isotretinoin is presently the primary choice for the treatment of acne vulgaris. However, it could induce several adverse reactions like diarrhea, cheilitis, headache, elevated triglyceride levels and risk of inflammatory bowel disease and depression. Hence, it is imperative to seek an alternative therapy.MethodsOne hundred five patients were randomly divided into 3 groups, and received a baseline treatment of oral doxycycline for the initial 4 weeks. Group I received isotretinoin oral for 12 weeks; Group P received oral Lactobacillus plantarum MH-301 treatment for 12 weeks; Group IP received combined treatment with oral probiotics and oral isotretinoin for 12 weeks. The number of skin lesions was recorded at 0, 4, 8, and 12 weeks during the treatment to compare the efficacy of each intervention, and skin and fecal samples were collected from patients at 12 weeks for high-throughput sequencing to explore the microbiota differences between various groups.ResultsOur results revealed that the combination of L. plantarum MH-301 with isotretinoin significantly reduced the number of skin lesions in patients compared to using L. plantarum MH-301 and isotretinoin alone (p ConclusionIn conclusion, L. plantarum MH-301 could be used in combination with isotretinoin for optimal results in the treatment of acne vulgaris. The research conducted provides theoretical and data support for the adjuvant effect of L. plantarum in the treatment of acne vulgaris.Clinical Trial Registration[ClinicalTrials.gov], identifier (ChiCTR2200063499).</p

DataSheet1_Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization.docx

Critical limb ischemia (CLI) is associated with a higher risk of limb amputation and cardiovascular death. Dapagliflozin has shown great potential in the treatment of cardiovascular disease. However, the effects of dapagliflozin on CLI and the underlying mechanisms have not been fully elucidated. We evaluated the effect of dapagliflozin on recovery from limb ischemia using a mouse model of hindlimb ischemia. The flow of perfusion was evaluated using a laser Doppler system. Tissue response was assessed by analyzing capillary density, arterial density, and the degree of fibrosis in the gastrocnemius muscle. Immunofluorescence and Western blot were used to detect the expression of macrophage polarization markers and inflammatory factors. Our findings demonstrate the significant impact of dapagliflozin on the acceleration of blood flow recovery in a hindlimb ischemia mouse model, concomitant with a notable reduction in limb necrosis. Histological analysis revealed that dapagliflozin administration augmented the expression of key angiogenic markers, specifically CD31 and α-SMA, while concurrently mitigating muscle fibrosis. Furthermore, our investigation unveiled dapagliflozin’s ability to induce a phenotypic shift of macrophages from M1 to M2, thereby diminishing the expression of inflammatory factors, including IL-1β, IL-6, and TNF-α. These effects were partially mediated through modulation of the NF-κB signaling pathway. Lastly, we observed that endothelial cell proliferation, migration, and tube-forming function are enhanced in vitro by utilizing a macrophage-conditioned medium derived from dapagliflozin treatment. Taken together, our study provides evidence that dapagliflozin holds potential as an efficacious therapeutic intervention in managing CLI by stimulating angiogenesis, thereby offering a novel option for clinical CLI treatment.</p

Data_Sheet_1_Lactobacillus plantarum MH-301 as an effective adjuvant to isotretinoin in the treatment of acne vulgaris: a randomized and open-label trail.docx

IntroductionAcne vulgaris is a common chronic inflammatory skin disease originating in the sebaceous gland units of the skin follicles. Isotretinoin is presently the primary choice for the treatment of acne vulgaris. However, it could induce several adverse reactions like diarrhea, cheilitis, headache, elevated triglyceride levels and risk of inflammatory bowel disease and depression. Hence, it is imperative to seek an alternative therapy.MethodsOne hundred five patients were randomly divided into 3 groups, and received a baseline treatment of oral doxycycline for the initial 4 weeks. Group I received isotretinoin oral for 12 weeks; Group P received oral Lactobacillus plantarum MH-301 treatment for 12 weeks; Group IP received combined treatment with oral probiotics and oral isotretinoin for 12 weeks. The number of skin lesions was recorded at 0, 4, 8, and 12 weeks during the treatment to compare the efficacy of each intervention, and skin and fecal samples were collected from patients at 12 weeks for high-throughput sequencing to explore the microbiota differences between various groups.ResultsOur results revealed that the combination of L. plantarum MH-301 with isotretinoin significantly reduced the number of skin lesions in patients compared to using L. plantarum MH-301 and isotretinoin alone (p ConclusionIn conclusion, L. plantarum MH-301 could be used in combination with isotretinoin for optimal results in the treatment of acne vulgaris. The research conducted provides theoretical and data support for the adjuvant effect of L. plantarum in the treatment of acne vulgaris.Clinical Trial Registration[ClinicalTrials.gov], identifier (ChiCTR2200063499).</p

The “Pure Marriage” between 3D Printing and Well-Ordered Nanoarrays by Using PEALD Assisted Hydrothermal Surface Engineering

For the first time, homogeneous and well-ordered functional nanoarrays were grown densely on the complex structured three-dimensional (3D) printing frameworks through a general plasma enhanced atomic layer deposition (PEALD) assisted hydrothermal surface engineering process. The entire process was free from toxic additives or harmful residues and, therefore, can meet the critical requirements of high-purity products. As a practical example, 3D customized earplugs were precisely manufactured according to the model of ear canals at the 0.1 mm level. Meanwhile, well-ordered ZnO nanoarrays, formed on the surfaces of these 3D printed earplugs, could effectively prevent the growth of five main pathogens derived from the patients with otitis media and exhibited excellent wear resistance as well. On the basis of both animal experiments and volunteers’ investigations, the 3D customized earplugs showed sound insulation capabilities superior to those of traditional earplugs. Further animal experiments demonstrated the potential of as-modified implant scaffolds in practical clinical applications. This work, exemplified with earplugs and implant scaffolds, oriented the development direction of 3D printing in biomedical devices, which precisely integrated customized architecture and tailored surface performance

The “Pure Marriage” between 3D Printing and Well-Ordered Nanoarrays by Using PEALD Assisted Hydrothermal Surface Engineering

For the first time, homogeneous and well-ordered functional nanoarrays were grown densely on the complex structured three-dimensional (3D) printing frameworks through a general plasma enhanced atomic layer deposition (PEALD) assisted hydrothermal surface engineering process. The entire process was free from toxic additives or harmful residues and, therefore, can meet the critical requirements of high-purity products. As a practical example, 3D customized earplugs were precisely manufactured according to the model of ear canals at the 0.1 mm level. Meanwhile, well-ordered ZnO nanoarrays, formed on the surfaces of these 3D printed earplugs, could effectively prevent the growth of five main pathogens derived from the patients with otitis media and exhibited excellent wear resistance as well. On the basis of both animal experiments and volunteers’ investigations, the 3D customized earplugs showed sound insulation capabilities superior to those of traditional earplugs. Further animal experiments demonstrated the potential of as-modified implant scaffolds in practical clinical applications. This work, exemplified with earplugs and implant scaffolds, oriented the development direction of 3D printing in biomedical devices, which precisely integrated customized architecture and tailored surface performance