Mesoporous Zeolite ZSM‑5 Supported Ni₂P Catalysts with High Activity in the Hydrogenation of Phenanthrene and 4,6-Dimethyldibenzothiophene

Mesoporous zeolite ZSM-5 (ZSM-5-M) was synthesized and used as support for the preparation of highly efficient nickel phosphide catalyst (Ni₂P/ZSM-5-M) in the deep hydrogenation of phenanthrene and in the hydrodesulfurization (HDS) of 4,6-dimethyldibenzothiophene (4,6-DM-DBT). Compared with Ni₂P catalysts supported silica and high surface area hexagonal mesoporous silica (HMS) (Ni₂P/SiO₂ and Ni₂P/HMS), Ni₂P/ZSM-5-M exhibits higher hydrogenation and HDS activity. The phenanthrene conversion and deep hydrogenation products selectivity over Ni₂P/ZSM-5-M (95% and 83%) are much higher than those over Ni₂P/SiO₂ (61% and 73%) and Ni₂P/HMS (69% and 45%) under mild conditions. The 4,6-DM-DBT conversion over Ni₂P/ZSM-5-M (93%) was higher than that over Ni₂P/SiO₂ (62%). This feature is attributed to the difference in surface properties of support. A large amount of acidic hydroxyl groups on the zeolites can interact strongly with catalyst precursor, resulting in the formation of highly dispersed Ni₂P particles with small sizes, which provide abundant hydrogenation active sites

Data_Sheet_1_New insights into the relationship between the average nucleotide identity and the digital DNA–DNA hybridization values in the genus Amycolatopsis and Amycolatopsis cynarae sp. nov., a novel actinobacterium from the rhizosphere soil of Cynara scolymus, and proposal of Amycolatopsis niigatensis as a synonym of Amycolatopsis echigonensis based on comparative genomic analysis.docx

At present, it is widely believed that a 95–96% average nucleotide identity (ANI) value is equivalent to a 70% digital DNA–DNA hybridization (dDDH) value in the prokaryotic taxonomy. However, in the present study, comparative genome analysis of 29 pairs of Amycolatopsis type strains revealed that a 70% dDDH value did not correspond to a 95–96% ANI based on the MuMmer ultra-rapid aligning tool (ANIm) but approximately corresponded to a 96.6% ANIm value in the genus Amycolatopsis. Based on this corresponding relationship, phenotypic and chemotaxonomical characteristics, as well as phylogenetic analysis, an actinobacterial strain HUAS 11-8T isolated from the rhizosphere soil of Cynara scolymus, was subjected to a polyphasic taxonomic characterization. Based on EzBioCloud alignment, it was found that strain HUAS11-8T had the 16S rRNA gene similarities of 99.78% with A. rhizosphaerae JCM 32589T, 97.8% with A. dongchuanensis YIM 75904T, and T was closely related to A. rhizosphaerae JCM 32589T. ANIm and dDDH values between strains HUAS 11-8T and A. rhizosphaerae JCM 32589T were 96.3 and 68.5%, respectively, lower than the 96.6 and 70% thresholds recommended for the delineation of a novel Amycolatopsis species. Consequently, strain HUAS 11-8T should represent a novel Amycolatopsis species, for which the name Amycolatopsis cynarae sp. nov. (type strain HUAS 11-8T = MCCC 1K08337T = JCM 35980T) is proposed. Furthermore, based on comparative genomic analysis and rule 42 of the Prokaryotic Code, we propose that Amycolatopsis niigatensis is a later heterotypic synonym of Amycolatopsis echigonensis.</p

A new biomarker to enhance the radiosensitivity of hepatocellular cancer: miRNAs Supplementary Tables 1-2

A new biomarker to enhance the radiosensitivity of hepatocellular cancer: miRNAs Supplementary materials</p

A new biomarker to enhance the radiosensitivity of hepatocellular cancer: miRNAs Supplementary references

A new biomarker to enhance the radiosensitivity of hepatocellular cancer: miRNAs</p

Synthesis of 3-Substituted Isocoumarins via a Cascade Intramolecular Ullmann-Type Coupling–Rearrangement Process

A simple and highly efficient strategy for the synthesis of 3-substituted isocoumarins through a copper­(I)-catalyzed reaction of 1-(2-halophenyl)-1,3-diones has been developed. The procedure is based on a cascade copper-catalyzed intramolecular Ullmann-type C-arylation and rearrangement process. This methodology is tolerant of a wide range of substrates and applicable to library synthesis

Palladium-Catalyzed Synthesis of Isocoumarins and Phthalides via <i>tert</i>-Butyl Isocyanide Insertion

A novel and highly efficient strategy for the synthesis of isocoumarins and phthalides through a palladium(0)-catalyzed reaction incorporating <i>tert</i>-butyl isocyanide has been developed. This process, providing one of the simplest methods for the synthesis of this class of valuable lactones, involves two steps including cyclization reaction and simple acid hydrolysis. The methodology is tolerant of a wide range of substrates and applicable to library synthesis

Summary of the <i>M. domestica</i> transcriptome.

<p>Summary of the <i>M. domestica</i> transcriptome.</p

RhlA Exhibits Dual Thioesterase and Acyltransferase Activities during Rhamnolipid Biosynthesis

Rhamnolipids (RLs) are a desirable class of biosurfactants originating from Pseudomonas aeruginosa. Rhamnosyltransferase 1 chain A (RhlA) catalyzes the formation of β-3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) to constitute the RL lipid moiety, and the molecular structure of this moiety exerts major impacts on the physiochemical and biological properties of corresponding RLs. However, the catalytic mechanism and sequence-structure–function relationship of RhlA remain elusive. Here, we report the X-ray crystal structure of P. aeruginosa RhlA with an α/β-hydrolase fold and a canonical nucleophile/histidine/acidic catalytic triad. Unexpectedly, free 3-hydroxy fatty acids within a secondary ligand-binding pocket were observed in the crystal of RhlA, which is traditionally considered an acyltransferase that acts only on acyl carrier protein (ACP)-bound substrates. In vitro isotopic labeling, enzyme kinetics experiments, and QM/MM simulations confirmed that free β-hydroxy fatty acids are a reaction intermediate during HAA synthesis. Moreover, first-shell residue mutations that targeted different ligand-binding pockets resulted in distinct modulation patterns for the two acyl chain lengths of HAAs. In conclusion, the revealed biosynthetic mechanism may guide future engineering for the biosynthesis of designer RLs

Classification of the gene ontology (GO) for the <i>M. domestica</i> transcriptome.

<p>7,063 unigenes (26.1% of total) were categorized into 48 function groups.</p

Differentially expressed genes of each library compared with CK.

<p>Unigenes changed at transcriptional level following bacterial challenge were identified by filtering of the one-fold up- and down-regulated ones with FDR≤0.001.</p