The CK8-specific particles were observed in the conjunctival epithelium cells.

<p>Immuno-electron microscopy shows that CK8-specific particles (indicated by white arrows) were observed in the multiple layers of conjunctival epithelium cells, and goblet cells (indicated by a white star) underneath the layer of conjunctival epithelium cells.</p

Methodology framework.

<p>Methodology framework.</p

Theoretical Elucidation of the Origin for Assembly of the DAP12 Dimer with Only One NKG2C in the Lipid Membrane

In this work, we have investigated in details the origin of the assembly of the DAP12 dimer with only one NKG2C in the activating immunoreceptor complex from thew two aspects of electronic properties and dynamic structures by performing density functional theory (DFT) calculations and molecular dynamics (MD) simulations. In the DFT calculations, we studied the aggregation ability of the NKG2CTM with the DAP12TM dimer and the DAP12TM–DAP12TM–NKG2CTM complex by analyzing the electrostatic potentials and frontier molecular orbitals (FMOs), and in the MD simulations we mainly investigated the dynamic structures of the DAP12TM–DAP12TM–NKG2CTM complex and its mutants, as well as the tetramer complex consisting of two DAP12TM and two NKG2CTM helixes without any restriction. Through the studies of the electrostatic potential, the FMOs, and the dynamic structures, we have provided reasonable explanations to some extent for the experimental observation that only one NKG2C can associate with the DAP12 homodimer. The present theoretical results are expected to give valuable information for further studying the assembly between receptors and signaling subunits

Additional file 2: of Prevalence of depression and anxiety in systemic lupus erythematosus: a systematic review and meta-analysis

Summaries of symptom thresholds required for diagnosis of depression/anxiety. (DOCX 19 kb

Additional file 4: of Prevalence of depression and anxiety in systemic lupus erythematosus: a systematic review and meta-analysis

Quality Assessment. (DOCX 19 kb

Polyfluoroalkyl Tag Decoration Enables Significantly Enhanced Tumor Penetration Ability of a PTK7 Targeting Aptamer

Aptamers are widely used molecular recognition tools in targeted therapy, but their ability to effectively penetrate deep into solid tumors remains a significant challenge, leading to suboptimal treatment efficacy. Here, we developed a polyfluoroalkyl (PFA) decoration strategy to enhance aptamer recognition, cell internalization, and solid tumor penetration. Our results indicate that PFA with around 11 fluorine atoms significantly improves aptamer internalization both in vitro and in vivo settings. However, we also observed that the use of PFA tags containing 19 and 23 fluorine atoms on aptamers resulted in nonspecific cell anchoring in control cell lines, affecting the specificity of aptamers. Overall, we found that using a chemical modification strategy could enhance the deep tumor penetration ability of aptamers and validate their effectiveness in vivo. This approach has significant practical applications in targeted drug delivery for cancer treatment

Additional file 1: of Prevalence of depression and anxiety in systemic lupus erythematosus: a systematic review and meta-analysis

Search Terms. (DOCX 10 kb

Data for: New intelligent photometric titration system and its method for constructing COD based on micro-flow injection

Photovoltage in the proces

Data for: New intelligent photometric titration system and its method for constructing COD based on micro-flow injection

Photovoltage in the proces