Blood pressure and ventricular function.

<p>Blood pressure (panel A) and ventricular function (panel B) were in the normal range in both B6 and D2 mice.</p

Heart weight and cardiomyocyte size.

<p>Left ventricle thickness (panel B) and cardiomyocyte size (panel D) were markedly increased in D2 mice compared to B6 mice (panels A and C, respectively). Heart weight (panel E) and cardiomyocyte diameter (panel F) were significantly enhanced in the D2 strain compared to the B6 strain. Panels C and D: x200</p

Echocardiography determination of cardiac dimensions.

<p>Compared to B6 mice, LVPWd (panel A), LVPWs (panel B), LVDd (panel E) and LVDVs (panel F) were significantly greater in D2 mice. There was no difference in LVAWd (panel C) and LVAWs (panel D) between B6 and D2 mice.</p

Cardiac hypertrophy in the D2 strain.

<p>As detected by RNA-seq, β-MHC, α1-actin, ANP and BNP mRNA levels were significantly elevated in D2 mice compared to B6 mice (panels A-D). RNA-seq data on ANP and α1-actin were validated by qPCR (panel E). Western blot analysis further revealed that β-MHC protein levels in the D2 strain were significantly greater than the B6 strain (panel F).</p

Polymorphism on <i>Myh7</i> and <i>Mybpc3</i> Genes between B6 and D2 Strains.

<p>Polymorphism on <i>Myh7</i> and <i>Mybpc3</i> Genes between B6 and D2 Strains.</p