Implementasi Algoritma K-Nearest Neighbour Untuk Menentukan Nomor Klasifikasi Buku Studi Kasus: Perpustakaan Universitas Katolik Musi Charitas)

Classification of library books is important to allow visitors in search of a book. The classification system in the library of the Catholic University of Charity Musi using guide books dewey decimal classification (DDC). The problem in this research is the difficulty in determining the classification number of new books. By utilizing the methods of Information Retrieval (IR) or retrieval of information, so in this study will build an application program for classification of library books. The method will be used to classify the book library is a method of k-nearest neighbor (k-NN). The application program classification of library books is built with training data from library books Musi-Caritas Catholic University and the test data is a new book. Applications are made capable of classifying new library book

Retinoic Acid-Activated Ndrg1a Represses Wnt/β-catenin Signaling to Allow <i>Xenopus</i> Pancreas, Oesophagus, Stomach, and Duodenum Specification

<div><p>How cells integrate multiple patterning signals to achieve early endoderm regionalization remains largely unknown. Between gastrulation and neurulation, retinoic acid (RA) signaling is required, while Wnt/β-catenin signaling has to be repressed for the specification of the pancreas, oesophagus, stomach, and duodenum primordia in <i>Xenopus</i> embryos. In attempt to screen for RA regulated genes in <i>Xenopus</i> endoderm, we identified a direct RA target gene, N-myc downstream regulated gene 1a (<i>ndrg1a</i>) that showed expression early in the archenteron roof endoderm and late in the developing pancreas, oesophagus, stomach, and duodenum. Both antisense morpholino oligonucleotide mediated knockdown of <i>ndrg1a</i> in <i>Xenopus laevis</i> and the transcription activator-like effector nucleases (TALEN) mediated disruption of <i>ndrg1</i> in <i>Xenopus tropicalis</i> demonstrate that like RA signaling, Ndrg1a is specifically required for the specification of <i>Xenopus</i> pancreas, oesophagus, stomach, and duodenum primordia. Immunofluorescence data suggest that RA-activated Ndrg1a suppresses Wnt/β-catenin signaling in <i>Xenopus</i> archenteron roof endoderm cells. Blocking Wnt/β-catenin signaling rescued Ndrg1a knockdown phenotype. Furthermore, overexpression of the putative Wnt/β-catenin target gene Atf3 phenocopied knockdown of Ndrg1a or inhibition of RA signaling, while Atf3 knockdown can rescue Ndrg1a knockdown phenotype. Lastly, the pancreas/stomach/duodenum transcription factor Pdx1 was able to rescue Atf3 overexpression or Ndrg1a knockdown phenotype. Together, we conclude that RA activated Ndrg1a represses Wnt/β-catenin signaling to allow the specification of pancreas, oesophagus, stomach, and duodenum progenitor cells in <i>Xenopus</i> embryos.</p></div

Box and Whisker Plots of HCV viral load distribution by related factors.

<p>(A) HCV viral load stratified by subtype 1b and 2a. (B) HCV viral load between ART naïve and ART treated subjects. (C) Distribution of HCV viral load by HIV viral load <1000 and >1000. (D) HCV viral load between CD4 counts <200 and CD4 counts >200.</p

Correlation of serum enzyme levels and HCV viral load.

<p>(A) Association of AST and HCV viral load in subjects infected with subtype 1b. (B) Association of ALT and HCV viral load in subjects infected with subtype 1b. (C) Association of AST and HCV viral load in subjects infected with subtype 2a. (D) Association of ALT and HCV viral load in subjects infected with subtype 2a.</p

Socio demographic characteristics of HIV mono-infected and HIV/HCV co-infected subjects in the study.

<p>Socio demographic characteristics of HIV mono-infected and HIV/HCV co-infected subjects in the study.</p

Pyrococcus furiosus Argonaute Based Detection Assays for Porcine Deltacoronavirus

Porcine deltacoronavirus (PDCoV) is a major cause of diarrhea and diarrhea-related deaths among piglets and results in massive losses to the overall porcine industry. The clinical manifestations of porcine diarrhea brought on by the porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and PDCoV are oddly similar to each other. Hence, the identification of different pathogens through molecular diagnosis and serological techniques is crucial. Three novel detection methods for identifying PDCoV have been developed utilizing recombinase-aided amplification (RAA) or reverse transcription recombinase-aided amplification (RT-RAA) in conjunction with Pyrococcus furiosus Argonaute (PfAgo): RAA-PfAgo, one-pot RT-RAA-PfAgo, and one-pot RT-RAA-PfAgo-LFD. The indicated approaches have a detection limit of around 60 copies/μL of PDCoV and do not cross-react with other viruses including PEDV, TGEV, RVA, PRV, PCV2, or PCV3. The applicability of one-pot RT-RAA-PfAgo and one-pot RT-RAA-PfAgo-LFD were examined using clinical samples and showed a positive rate comparable to the qPCR method. These techniques offer cutting-edge technical assistance for identifying, stopping, and managing PDCoV

Multivariate Logistic regression analysis of potential factors associated with HCV subtype 1b infection, compared to those with HCV subtype 2a infection (n = 88).

#<p>some subject have not donated.</p><p>*AOR adjusted for gender and age group.</p

Plant hormone concentrations in <i>pds2-1</i> and WT seedlings grown on ½ MS plates.

<p>Three-week-old seedlings were sampled to determine the levels of four plant hormones. P-values represent significant differences of the means between WT and <i>pds2-1</i> tissues (n = 3) after comparing them using Student's t-test. FW: fresh weight.</p

qRT-PCR analyses of genes involved in the carotenoid, GA and ABA biosynthetic pathways and in the regulation of trichome and root hair development.

<p>Significant differences of the means (± SD) between WT plants and <i>pds2-1</i> plants (n = 3) are indicated by * (P<0.05) and ** (P<0.01). The Arabidopsis <i>TUB2</i> gene was used as an internal control.</p

Expression analysis of the <i>HST</i> gene in WT Arabidopsis.

<p>(<b>A</b>) Tissue-specific expression as determined by qRT-PCR. The <i>UBQ10</i> gene was used as an internal control. Values are means ± SD (n = 3). Rt: roots; RL: rosette leaves; CL: cauline leaves; St: stems; Fl, flowers. (<b>B</b>) <i>HST</i> transcript levels at different leaf development stages. NS: no senescence; ES: early senescence; LS: late senescence. (<b>C–J</b>) Representative GUS expressions in HST_pro::<i>GUS</i> transgenic Arabidopsis. (<b>C</b>) 5-day-old etiolated seedling; (<b>D</b>) 15-day-old seedling; (<b>E</b>) mature leaf of a 4-week-old plant; (<b>F</b>) mature silique; (<b>G</b>) root; (<b>H</b>) inflorescence and flowers; (<b>I</b>) trichome from a stem; (<b>J</b>) leaves at different developmental stages.</p