3 research outputs found

    MOESM1 of Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source

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    Additional file 1: Table S1. Oligonucleotides used in this study. Table S2. P3HB production by E. coli strains cultivated in MM medium supplemented with CSL. Figure S1. Effect of acetate concentration on cell growth and P3HB production

    Site-Specific and High-Loading Immobilization of Proteins by Using Cohesin–Dockerin and CBM–Cellulose Interactions

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    Immobilization of enzymes enhances their properties for application in industrial processes as reusable and robust biocatalysts. Here, we developed a new immobilization method by mimicking the natural cellulosome system. A group of cohesin and carbohydrate-binding module (CBM)-containing scaffoldins were genetically engineered, and their length was controlled by cohesin number. To use green fluorescent protein (GFP) as an immobilization model, its C-terminus was fused with a dockerin domain. GFP was able to specifically bind to scaffoldin via cohesin–dockerin interaction, while the scaffoldin could attach to cellulose by CBM–cellulose interaction. Our results showed that this mild and convenient approach was able to achieve site-specific immobilization, and the maximum GFP loading capacity reached ∼0.508 μmol/g cellulose

    Co-fermentation of Cellulose and Sucrose/Xylose by Engineered Yeasts for Bioethanol Production

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    Consolidated bioprocessing (CBP) of cellulose mixed with fermentable sugar(s) is considered as a promising alternative to the use of cellulose as sole substrate for bioethanol production. Our research metabolically engineered Saccharomyces cerevisiae to allow for the co-conversion of cellulose and either sucrose or xylose to bioethanol. Constitutive promoter substitution and xylose metabolic pathway integration were carried out in a strain previously modified to express both bifunctional minicellulosomes by galactose induction and a cellodextrin pathway. Strain EBY101-CC, engineered for the co-fermentation of cellulose and sucrose, produced 4.3 g/L ethanol from 10 g/L carboxymethyl cellulose (CMC) and batch-fed sucrose with an ethanol yield of 0.43 g/g of total sugars. Strains modified for co-fermentation of xylose and cellulose, EBY101-X5CC and EBY101-X5CP were able to produce 2.9 g/L cellulosic ethanol from 8.0 g/L CMC and 1.2 g/L from 3.2 g/L phosphoric acid-swollen cellulose (PASC), respectively, when xylose was depleted
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