3 research outputs found
MOESM1 of Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source
Additional file 1: Table S1. Oligonucleotides used in this study. Table S2. P3HB production by E. coli strains cultivated in MM medium supplemented with CSL. Figure S1. Effect of acetate concentration on cell growth and P3HB production
Site-Specific and High-Loading Immobilization of Proteins by Using Cohesin–Dockerin and CBM–Cellulose Interactions
Immobilization of enzymes enhances
their properties for application
in industrial processes as reusable and robust biocatalysts. Here,
we developed a new immobilization method by mimicking the natural
cellulosome system. A group of cohesin and carbohydrate-binding module
(CBM)-containing scaffoldins were genetically engineered, and their
length was controlled by cohesin number. To use green fluorescent
protein (GFP) as an immobilization model, its C-terminus was fused
with a dockerin domain. GFP was able to specifically bind to scaffoldin
via cohesin–dockerin interaction, while the scaffoldin could
attach to cellulose by CBM–cellulose interaction. Our results
showed that this mild and convenient approach was able to achieve
site-specific immobilization, and the maximum GFP loading capacity
reached ∼0.508 μmol/g cellulose
Co-fermentation of Cellulose and Sucrose/Xylose by Engineered Yeasts for Bioethanol Production
Consolidated
bioprocessing (CBP) of cellulose mixed with fermentable sugar(s) is
considered as a promising alternative to the use of cellulose as sole
substrate for bioethanol production. Our research metabolically engineered Saccharomyces cerevisiae to allow for the co-conversion
of cellulose and either sucrose or xylose to bioethanol. Constitutive
promoter substitution and xylose metabolic pathway integration were
carried out in a strain previously modified to express both bifunctional
minicellulosomes by galactose induction and a cellodextrin pathway.
Strain EBY101-CC, engineered for the co-fermentation of cellulose
and sucrose, produced 4.3 g/L ethanol from 10 g/L carboxymethyl cellulose
(CMC) and batch-fed sucrose with an ethanol yield of 0.43 g/g of total
sugars. Strains modified for co-fermentation of xylose and cellulose,
EBY101-X5CC and EBY101-X5CP were able to produce 2.9 g/L cellulosic
ethanol from 8.0 g/L CMC and 1.2 g/L from 3.2 g/L phosphoric acid-swollen
cellulose (PASC), respectively, when xylose was depleted