Supplementary Data File 1 (GLMMdata).txt from Behavioural evidence for polychromatic ultraviolet sensitivity in mantis shrimp

Supplementary Data File 1 (GLMMdata). The .TXT data file is a tab delimited text file containing the responses and stimulus conditions for all choices made in the trained choice tests. This data set was used to assess the effect of brightness on individual choices in each of the experiments using generalized linear mixed model (GLMM). Column 1 (ans.): 1, correct choice; 0, incorrect choice. Column 2 (ind#): The number designation of each individual used in the experiments. See Table S1. Column 3 (exp.) designates the experiment: 1, UVA vs. UVB; 2, UVB vs. UVA; 3, UVB vs. dark; 4, dark vs. UVB 5, UVA vs. UVA-351; 6, UVA vs. UVA control. Column 4 (bright) designates the relative brightness of the correct stimulus. For experiments 1, 2, 5, and 6: 1, alternate stimulus brighter; 2, correct and alternate stimulus equivalent; 3, correct stimulus brighter. For experiments 3 and 4, where only the correct stimulus was illuminated, the data references the intensity of the correct stimulus, with 5 being the maximum brightness setting

Supplemental Materials from Behavioural evidence for polychromatic ultraviolet sensitivity in mantis shrimp

Includes 1 table, 3 figures

Cichlid opsin expression plasticity qPCR data

Cone opsin expression levels determined by qPCR relative to GNAT2

<i>D</i>. <i>pealeii</i> paralarval parolfactory vesicle immunolabeled with anti-rhodopsin, anti-retinochrome, and anti-acetylated α-tubulin antibodies.

<p>(A) Rhodopsin (green) is present throughout the parolfactory vesicles while (B) retinochrome (red) and α-tubulin (white) are present in discrete cell bodies. (C) Overlap of rhodopsin, retinochrome, and α-tubulin labeling appears white. Blue shows autofluorescence of the tissue. Scale bar, 25μm.</p

Immunolabeling in <i>D</i>. <i>pealeii</i> paralarval arms shows presence of rhodopsin and retinochrome in each arm ganglion.

<p>(A) Rhodopsin (green) labeling only; (B) retinochrome (red) labeling only. (C) Overlap of rhodopsin and retinochrome labeling occurs at the junction between arm and each sucker, and appears yellow. Small cell bodies also labeled with rhodopsin and retinochrome are hair cells (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135381#pone.0135381.g006" target="_blank">Fig 6</a>). Blue shows autofluorescence of the tissue. Scale bar, 25μm.</p

LWS in situ hybridization probe sequence

LWS in situ hybridization probe sequenc

Hair cells in <i>D</i>. <i>pealeii</i> hatchlings immunolabeled with rhodopsin and retinochrome-specific antibodies.

<p>(A) Rhodopsin (green) labeling only; (B) retinochrome (red) labeling only. (C) Overlap of rhodopsin and retinochrome labeling in hair cell cell bodies appears yellow. Acetylated α-tubulin antibody (white) labels the cell body and cilia bundle protruding from the cell body (inset). Blue shows autofluorescence of the tissue. Scale bar, 25μm.</p

Immunolabeling of rhodopsin, retinochrome, and acetylated α-tubulin in a whole mount of <i>D</i>. <i>pealeii</i> retina.

<p>(A) Rhodopsin (green) is present only in outer segments, represented by regions parallel to the solid line. Rhodopsin is seen predominantly on the tips and bases of outer segments due to dense packing of visual and screening pigments, which prevent binding in parts of the outer segments. (B) Retinochrome (red) is present only in the inner segments, indicated by the dashed line. (C) Acetylated α-tubulin (white) is present in the inner segments. Blue shows autofluorescence of the tissue. Scale bar, 25μm.</p

<i>D</i>. <i>pealeii</i> dorsal mantle tissue immunolabeled with anti-rhodopsin and anti-retinochrome, and imaged in the chromatophore layer.

<p>(A) Rhodopsin (green) labeling only; (B) retinochrome (red) labeling only. (C) Overlap of rhodopsin and retinochrome labeling in the chromatophore membrane (m), radial muscle fibers (r), and sheath cells (sc) appears yellow. Blue shows autofluorescence of the tissue. Scale bar, 25μm.</p

RH2B in situ hybridization probe sequence

RH2B in situ hybridization probe sequenc