Photochemistry of Benzophenone Immobilized in a Major Groove of DNA: Formation of Thermally Reversible Interstrand Cross-link

We here report a highly site and sequence selective formation of an interstrand cross-link of BPU-containing oligomer duplexes. The cross-link was found spontaneously reverted to original oligomers upon heating, providing a new method for the temporary connection of two DNA strands

Data_Sheet_1_Cell Type Specific Representation of Vibro-tactile Stimuli in the Mouse Primary Somatosensory Cortex.docx

Although the processing of whisker deflections in the barrel area of the rodent primary somatosensory cortex (S1) has been studied extensively, how cutaneous vibro-tactile stimuli are processed in the rodent S1 outside the barrel area has not been fully examined. Particularly, the cell-type specific representation of multiple vibration frequencies in genetically identified inhibitory cells in the S1 has not been examined. Using two-photon calcium imaging, we examined the responses to vibration stimuli of excitatory and inhibitory neurons in the S1 hind limb area of male and female mice. The excitatory cells showed relatively sharp selectivity to vibration stimuli, whereas the inhibitory cells exhibited less selectivity. The excitatory and inhibitory cells with different preferred stimuli were intermingled in a “salt and pepper” manner. Furthermore, the noise correlation tended to be especially strong in excitatory-inhibitory and inhibitory-inhibitory cell pairs that have similar stimulus selectivity. These results suggest that excitatory cells tend to represent specific stimulus information and work together with similarly tuned inhibitory cells as a functionally connected network.</p

Variable selection by graphical modeling.

The graphical modeling was performed using the R packages “corpcor” [26] and “qgraph” [27].</p

Diarylheptanoid Sulfates and Related Compounds from <i>Myrica rubra</i> Bark

Three new diarylheptanoids, myricanol 11-sulfate (<b>1</b>), juglanin B 11-sulfate (<b>2</b>), and myricanone 5-<i>O</i>-(6′-<i>O</i>-galloyl)­glucoside (<b>3</b>), were isolated from the bark of <i>Myrica rubra</i>. Compounds <b>1</b> and <b>2</b> were characterized as diarylheptanoid sulfates on the basis of spectroscopic analyses. The antioxidative activities of the fractionated extracts and isolated compounds were estimated by the oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD)-like activity assays. The major isolate, myricitrin (<b>4</b>), displayed a high ORAC value and moderate SOD-like activity (13 198 μmol TE (Trolox equivalent)/g and IC₅₀ 127.5 μg/mL, respectively), which might explain the potent antioxidative activity of this material

Odds ratio of each variable estimated by the logistic regression model.

Odds ratio of each variable estimated by the logistic regression model.</p

Cypellocarpins A−C, Phenol Glycosides Esterified with Oleuropeic Acid, from <i>Eucalyptus cypellocarpa</i>

Three new phenol glycosides acylated with (+)-oleuropeic acid, cypellocarpins A (1), B (2), and C (3), along with seven known compounds, were isolated from the dried leaves of Eucalyptus cypellocarpa. Structures of the new compounds were determined on the basis of spectroscopic methods, including 2D NMR experiments and chemical degradation. These new compounds and a known related glucoside (7) showed potent in vitro antitumor-promoting activity in a short-term bioassay evaluating the inhibitory effect on Epstein−Barr virus early antigen activation induced by 12-O-tetradecanoyl phorbol 13-acetate (TPA). These compounds also suppressed an in vivo two-stage carcinogenesis induced with nitric oxide and TPA on mouse skin

Summary of the logistic regression model using selected variables.

Summary of the logistic regression model using selected variables.</p

ROC curves using all the investigated models.

LogReg: logistic regression analysis. NB: Naive Bayes classifier. RF: random forest. SVM: support vector machine. The ROC curves were plotted using the R packages “plotROC” [32] and “ggplot2” [33].</p

Typical examples of the presence or absence of cerebral white matter lesions.

The upper three images are those of a subject with cerebral white matter lesions, while the lower three images are those of a subject without cerebral white matter lesions. T1 weighted images (T1WI), T2 weighted images (T2WI), and Fluid Attenuated Inversion Recovery (FLAIR) images were obtained using MRI equipment (MAGNETOM Symphony and MAGNETOM ESSENZA; Siemens Healthineers Global), but only the FLAIR images are shown here.</p

A ROC curves using the final logistic discriminant model.

A point on the ROC curve shows the most appropriate cutoff value giving by the Youden Index.</p