MOESM1 of Host, pathogen and environment: a bacterial gbpA gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin

Additional file 1. Vibrio parahaemolyticus PCV08-7 details for the three experimental setups MgSO4·7H2O, with MgSO4·7H2O and Chitin, with MgSO4·7H2O and carapace. Figure S1: Vibrio parahaemolyticus PCV08-7 culture plates for the three experimental setups MgSO4·7H2O (AppFigure1), with MgSO4·7H2O and Chitin (AppFigure2), with MgSO4·7H2O and carapace (AppFigure3). Table S1: RNA and cDNA concentrations for the three experimental setups MgSO4·7H2O (AppTable1), with MgSO4·7H2O and Chitin (AppTable2), with MgSO4·7H2O and carapace (AppTable3), Table S2: Analysis of variance for the significant values of the experimental setup MgSO4·7H2O, Table S3: Analysis of variance for the significant values of the experimental setup MgSO4·7H2O and Chitin, Table S4: Analysis of variance for the significant values of the experimental setup MgSO4·7H2O and carapace, Table S5: Student two tail paired t-test of equal variance across the three experimental setups MgSO4·7H2O, with MgSO4·7H2O and Chitin, with MgSO4·7H2O and carapace

MOESM1 of Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Additional file 1. Fasta sequence of the Vibrio parahaemolyticus GbpA protein

Variance analysis showing qualitative variability in TRs and BRs of three H1993, A549, and H1975 cell line exosomes.

Variance analysis showing qualitative variability in TRs and BRs of three H1993, A549, and H1975 cell line exosomes.</p

Heatmap showing number of samples in which each of the total 886 proteins was identified in H1993 exosomes.

Proteins common to all nine replicates are shown in blue (117). Proteins present in >1 replicate and <9 replicates are shown in grey (312) and proteins present only in 1 replicate are shown in red (457).</p

Variability in protein cargo detection in technical and biological replicates of exosome-enriched extracellular vesicles - Fig 2

Venn diagrams and tables showing the average number of proteins (in total and in percentage of the total) identified across three TRs and three BRs out of the total proteins detected in the three cell lines H1993 (886), A549 (976) and H1975 (879), respectively for (A) TRs (B) BRs. The proteins unique to the three replicates is shown in pink, the proteins shared by 2 replicates is shown in yellow, and the proteins common to three replicates is shown in blue.</p

Schematic representation of exosomal samples collection.

Exosomes were collected from three different cell lines at three different passages (A, B, C) to provide three biological replicates (BRs). The exosome lysate from each of them was further divided into three technical replicates (TRs). The nine samples were grouped so that the TRs were TR1: R1, R2, R3, TR2: R4, R5, R6, TR3: R7, R8, and R9. The BRs were BR1: R1, R4, R7, B2: R2, R5, R8, BR3: R3, R6, and R9.</p

Heatmap showing number of samples in which each of the total 976 proteins was identified in A549 exosomes.

Proteins common to all nine replicates are shown in blue (223). Proteins present in >1 replicate and <9 replicates are shown in grey (359) and proteins present only in 1 replicate are shown in red (394).</p

Heatmap representing exosomal proteins identified in H1975 in order of decreasing levels in TRs (R1-R2-R3, R4-R5-R6, R7-R8-R9) and in BRs (R1-R4-R7, R2-R5-R6, R3-R6-R9).

Boxes highlighted in dark red represent the most abundant, while boxes in dark blue represent the least abundant proteins.</p

Heatmap representing exosomal proteins identified in H1993 in order of decreasing levels in TRs (R1-R2-R3, R4-R5-R6, R7-R8-R9) and in BRs (R1-R4-R7, R2-R5-R6, R3-R6-R9).

Boxes highlighted in dark red represent the most abundant, while boxes in dark blue represent the least abundant proteins.</p

Heatmap showing number of samples in which each of the total 879 proteins was identified in H1975 exosomes.

Proteins common to all nine replicates are shown in blue (108). Proteins present in >1 replicate and <9 replicates are shown in grey (305) and proteins present only in 1 replicate are shown in red (466).</p