sj-docx-1-wjn-10.1177_01939459241230388 – Supplemental material for Development and Testing of the School Healthcare Partnership Scale for Parents

Supplemental material, sj-docx-1-wjn-10.1177_01939459241230388 for Development and Testing of the School Healthcare Partnership Scale for Parents by Ju-Yeon Uhm and Suhee Kim in Western Journal of Nursing Research</p

Direct Detection of α‑1 Antitrypsin in Serum Samples using Surface Plasmon Resonance with a New Aptamer–Antibody Sandwich Assay

The challenges associated with performing surface plasmon resonance (SPR) based measurements in serum and other biofluids have continued to limit the applicability of this valuable sensing technology for sensitive bioaffinity measurements of proteins in clinically relevant samples. In this paper, a new sandwich assay is introduced for the quantitative SPR analysis of α-1 antitrypsin (AAT), which is a recognized biomarker for Alzheimer’s disease. Detection was performed via the specific adsorption of AAT onto a gold chip surface modified with a DNA aptamer. The measurement dynamic range and also sensitivity in serum were improved with the subsequent surface binding of antiAAT. A methodology was established to measure the target protein in serum, albumin and immunoglobulin G (IgG) solutions with the results correlated with measurements in buffer only. A comparison between SPR and enzyme-linked immunosorbent assay (ELISA) measurements was also made. The detection of AAT in serum at clinically relevant concentrations was demonstrated with target concentrations as low as 10 fM readily achievable

sj-docx-1-ras-10.1177_00208523211043362 - Supplemental material for The effects of performance evaluation on punishment in organisations

Supplemental material, sj-docx-1-ras-10.1177_00208523211043362 for The effects of performance evaluation on punishment in organisations by Seungwon Yu, Eun Ji Yoo and Suhee Kim in International Review of Administrative Sciences</p

Modification of cysteine 457 in plakoglobin modulates the proliferation and migration of colorectal cancer cells by altering binding to E-cadherin/catenins

Objectives: In tissue samples from patients with colorectal cancer (CRC), oxidation of C420 and C457 of plakoglobin (Pg) within tumor tissue was identified by proteomic analysis. The aim of this study was to identify the roles of Pg C420 and C457. Methods: Human CRC tissues, CRC and breast cancer cells, and normal mouse colon were prepared to validate Pg oxidation. MC38 cells were co-transfected with E-cadherin plus wild type (WT) or mutant (C420S or C457S) Pg to evaluate protein interactions and cellular localization, proliferation, and migration. Results: Pg was more oxidized in stage III CRC tumor tissue than in non-tumor tissue. Similar oxidation of Pg was elicited by H2O2 treatment in normal colon and cancer cells. C457S Pg exhibited diminished binding to E-cadherin and α-catenin, and reduced the assembly of E-cadherin–α-/β-catenin complexes. Correspondingly, immunofluorescent analysis of Pg cellular localization suggested impaired binding of C457S Pg to membranes. Cell migration and proliferation were also suppressed in C457S-expressing cells. Discussion: Pg appears to be redox-sensitive in cancer, and the C457 modification may impair cell migration and proliferation by affecting its interaction with the E-cadherin/catenin axis. Our findings suggest that redox-sensitive cysteines of Pg may be the targets for CRC therapy.</p

Boosting Charge Transfer Efficiency by Nanofragment MXene for Efficient Photoelectrochemical Water Splitting of NiFe(OH)<i>_x</i> Co-Catalyzed Hematite

The use of oxygen evolution co-catalysts (OECs) with hematite photoanodes has received much attention because of the potential to reduce surface charge recombination. However, the low surface charge transfer and bulk charge separation rate of hematite are not improved by decorating with OECs, and the intrinsic drawbacks of hematite still limit efficient photoelectrochemical (PEC) water splitting. Here, we successfully overcame the sluggish oxygen evolution reaction performance of hematite for water splitting by inserting zero-dimensional (0D) nanofragmented MXene (NFMX) as a hole transport material between the hematite and the OEC. The 0D NFMX was fabricated from two-dimensional (2D) MXene sheets and deposited onto the surface of a three-dimensional (3D) hematite photoanode via a centrifuge-assisted method without altering the inherent performance of the 2D MXene sheets. Among many OECs, NiFe­(OH)x was selected as the OEC to improve hematite PEC performance in our system because of its efficient charge transport behavior and high stability. Because of the great synergy between NFMX and NiFe­(OH)x, NiFe­(OH)x/NFMX/Fe2O3 achieved a maximum photocurrent density of 3.09 mA cm–2 at 1.23 VRHE, which is 2.78-fold higher than that of α-Fe2O3 (1.11 mA cm–2). Furthermore, the poor stability of MXene in an aqueous solution for water splitting was resolved by uniformly coating it with NiFe­(OH)x, after which it showed outstanding stability for 60 h at 1.23 VRHE. This study demonstrates the successful use of NFMX as a hole transport material combined with an OEC for highly efficient water splitting

Stress-Relief Network in Silicon Microparticles and Composite Anodes for Durable High-Energy-Density Batteries

Silicon microparticles (SiMPs), which have a high capacity, a high initial Coulombic efficiency, and a low volume-to-surface ratio compared with nanosized materials, are promising anode materials for high-energy-density battery applications. However, SiMPs suffer from inevitable particle pulverization and electrode failure at the early cycle. In this study, we suggest the construction of a porous, stress-relief carbon network on the surface of each SiMP to alleviate particle degradation at the electrode level through a template-free co-reaction of thermal polymer pyrolysis and graphitization. The designed porous graphitic carbon network (pGN) structure features not only considerable electrical conductivity and expansion tolerance but also sturdy SiMP interconnection during cycling. This enables SiMPs to improve battery performance and achieve high Coulombic efficiency and a stable cycle life in fast-charging systems without particle dissipation. Moreover, the composite anode comprising a practical level of commercial graphite and SiMP contents with pGN operates effectively because of high cycle efficiency and structural integrity, which promises the realization of advanced battery applications

DataSheet_1_Peptoniphilus gorbachii alleviates collagen-induced arthritis in mice by improving intestinal homeostasis and immune regulation.docx

IntroductionThe intricate connection between gut microbiota and rheumatoid arthritis (RA) pathogenesis has gained prominence, although the specific microbial species contributing to RA development remain largely unknown. Recent studies have sought to comprehensively explore alterations in the human microbiome, focusing on identifying disease-related microbial species through blood analysis. Consequently, this study aimed to identify RA-associated microbial species using a serum microbial array system and to investigate the efficacy and underlying mechanisms of potential microbial species for RA treatment.MethodsSerum immunoglobulin M levels against 384 intestinal microbial species were assessed using a microbial microarray in patients with RA and healthy individuals. We investigated the therapeutic potential of the identified microbial candidate regarding arthritis development, immune responses, gut barrier function, and gut microbiome using a collagen-induced arthritis (CIA) mouse model.ResultsOur findings revealed significant alterations in antibody levels against 36 microbial species in patients with RA compared to healthy individuals. Notably, the antibody levels against Peptoniphilus gorbachii (PG) were decreased in patients with RA and exhibited an inverse correlation with RA disease activity. In vitro experiments demonstrated that PG produced acetate and butyrate, while exhibiting anti-inflammatory properties. In CIA mice, PG administration suppressed arthritis symptoms, reduced the accumulation of inflammatory monocytes in the mesenteric lymph nodes, and downregulated gene expression of pro-inflammatory cytokines in the ileum. Additionally, PG supplementation restored intestinal barrier integrity and partially resolved gut microbial dysbiosis in CIA mice. The fecal microbiota in PG-treated mice corresponded to improved intestinal barrier integrity and reduced inflammatory responses.ConclusionThis study highlights the potential of serum-based detection of anti-microbial antibodies to identify microbial targets at the species level for RA treatment. Moreover, our findings suggest that PG, identified through the microbial microarray analysis, holds therapeutic potential for RA by restoring intestinal barrier integrity and suppressing the immunologic response associated with RA.</p

Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva

<div><p>The periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RNA sequencing and verified by real-time PCR. A total of 1939 mRNA transcripts showed significantly differential expression between young and old gingival tissues. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. In vitro experiments using human gingival fibroblasts (hGFs) showed that MMP12 was upregulated in old hGFs compared to young hGFs. Moreover, the MMP3, MMP9 and IL1B levels were more highly stimulated by infection with the oral bacterium, <i>Fusobacterium nucleatum</i>, in old hGFs compared to young hGFs. Collectively, these findings suggest that, in gingiva, the upregulation of MMP12 may be a molecular hallmark of natural aging, while the upregulations of MMP3, MMM9, and IL1B may indicate externally (e.g., infection)-induced aging. These findings contribute to our understanding of the molecular targets involved in gingival aging.</p></div

Analysis of gene transcripts in young and old gingival tissues using RNA sequencing.

<p>(A) Genes with significantly differential expression between young and old gingival tissues were shown as scatter plot. Blue lines represent baseline of upregulated and downregulated genes in old gingival tissues versus young gingival tissues in the range of ≥ ±2 fold differences, respectively. (B) Hierarchically heat map of differential expressed genes was presented for each individuals. Highly expressed genes in young or old were displayed as red, while lower expressed genes as green. (C) Ingenuity Pathway Analysis (IPA) were performed among the DEGs for pathways, upstream regulators, diseases and bio functions. Top 5 ranking per each categories is shown as enrichment score (−log10 (<i>p</i> value)). Y, young gingival tissues; and O, old gingival tissues.</p

Network of genes co-expressed with MMPs.

<p>(A) Genes that were co-regulated with the MMPs that were upregulated in old gingival tissues (red diamond shapes) were analyzed using IPA. Highly expressed genes in old gingival tissue were filled with red, while lower expressed genes as green. Genes filled with white indicate no significant difference between young and old gingival tissues, but are incorporated into the network through relationships with other molecules. (B) The several genes (IL1, JNK, and PDGF BB complexes) that were associated with MMP regulation were identified in old gingival tissues using real-time PCR. Y, young gingival tissues; and O, old gingival tissues; n = 5; and *<i>p</i> < 0.05 versus the results from Y.</p