sj-docx-1-wmr-10.1177_0734242X231155102 – Supplemental material for Exploring bioleaching potential of indigenous <i>Bacillus sporothermodurans</i> ISO1 for metals recovery from PCBs through sequential leaching process

Supplemental material, sj-docx-1-wmr-10.1177_0734242X231155102 for Exploring bioleaching potential of indigenous Bacillus sporothermodurans ISO1 for metals recovery from PCBs through sequential leaching process by Pooja Thakur and Sudhir Kumar in Waste Management & Research</p

sj-docx-4-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-4-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p

Image_1_PfHMGB2 has a role in malaria parasite mosquito infection.tif

Differentiation of asexually replicating parasites into gametocytes is critical for successful completion of the sexual phase of the malaria parasite life cycle. Gametes generated from gametocytes fuse to form a zygote which differentiates into ookinetes and oocysts. The sporozoites are formed inside oocysts which migrate to the salivary glands for next cycle of human infection. These morphologically and functionally distinct stages require stage-specific gene expression via specific transcriptional regulators. The capacity of high mobility group box (HMGB) proteins to interact with DNA in a sequence independent manner enables them to regulate higher order chromosome organization and regulation of gene expression. Plasmodium falciparum HMGB2 (PfHMGB2) shows a typical L- shaped predicted structure which is similar to mammalian HMG box proteins and shows very high protein sequence similarity to PyHMGB2 and PbHMGB2. Functional characterization of PfHMGB2 by gene deletion (Pfhmgb2¯) showed that knockout parasites develop normally as asexual stages and undergo gametocytogenesis. Transmission experiments revealed that Pfhmgb2¯ can infect mosquitoes and develop as oocyst stages. However, transmission was reduced compared to wild type (WT) parasites and as a consequence, the salivary gland sporozoites were reduced in number. In summary, we demonstrate that PfHMGB2 has no role in asexual growth and a modest role in sexual phase development and parasite transmission to the mosquito.</p

Table_1_PfHMGB2 has a role in malaria parasite mosquito infection.docx

Differentiation of asexually replicating parasites into gametocytes is critical for successful completion of the sexual phase of the malaria parasite life cycle. Gametes generated from gametocytes fuse to form a zygote which differentiates into ookinetes and oocysts. The sporozoites are formed inside oocysts which migrate to the salivary glands for next cycle of human infection. These morphologically and functionally distinct stages require stage-specific gene expression via specific transcriptional regulators. The capacity of high mobility group box (HMGB) proteins to interact with DNA in a sequence independent manner enables them to regulate higher order chromosome organization and regulation of gene expression. Plasmodium falciparum HMGB2 (PfHMGB2) shows a typical L- shaped predicted structure which is similar to mammalian HMG box proteins and shows very high protein sequence similarity to PyHMGB2 and PbHMGB2. Functional characterization of PfHMGB2 by gene deletion (Pfhmgb2¯) showed that knockout parasites develop normally as asexual stages and undergo gametocytogenesis. Transmission experiments revealed that Pfhmgb2¯ can infect mosquitoes and develop as oocyst stages. However, transmission was reduced compared to wild type (WT) parasites and as a consequence, the salivary gland sporozoites were reduced in number. In summary, we demonstrate that PfHMGB2 has no role in asexual growth and a modest role in sexual phase development and parasite transmission to the mosquito.</p

Sensitivity analysis and retrieval of optimum SLEUTH model parameters

The Cellular Automata (CA) based SLEUTH model has emerged as a widely applied model to many cities for land use land cover (LULC) change and urban growth modelling due to its simplicity, robustness, and ease of implementation. The present study employed a rigorous sensitivity testing of self-modifying constants, Monte Carlo runs and critical slope to determine their influence on model calibration performance. Calibration performance has been examined in terms of statistical measures i.e., urban area, clusters, edges, mean cluster size, and cluster radius, best model fitness measure (i.e., Optimal SLEUTH Metrics (OSM)), overall accuracy percentage and hit-miss-false alarm method have been used. The sensitivity analysis reveals the optimum values for self-modifying parameters as {1.3, 0.10, 0.90, and 1.25} for boom, bust, critical low and critical high respectively; Monte Carlo runs as sixty (60) and critical slope as 15 to simulate the urban growth of the study area.</p

sj-docx-7-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-7-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p

sj-docx-12-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-12-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p

sj-docx-1-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-1-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p

sj-docx-3-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-3-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p

sj-docx-11-pie-10.1177_09544089221131666 - Supplemental material for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool

Supplemental material, sj-docx-11-pie-10.1177_09544089221131666 for Animal waste powder utilization for Al6061/SiC composite using friction stir processing with scroll shoulder hexagonal tool by Prateek Gupta, Hari Singh and Sudhir Kumar in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p