Despite a 20-fold change in the repressor association rate, , the protein distributions derived from the analytical expression (31) (grey squares) are in good agreement with those obtained from stochastic simulations of the model (black disks).

<p>(a) Parameter values in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>. (b) is 1/10th of the value in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>; other parameter values as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>. (c) is 1/20th of the value in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>; other parameter values as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>.</p

Magnitudes of important derived parameters in the absence of the inducer.

<p>Magnitudes of important derived parameters in the absence of the inducer.</p

Parameter values in the absence of inducer.

<p>Parameter values in the absence of inducer.</p

The variation of the Fano factor and the reciprocal of the noise with the inducer level [12].

<p>(a) Derived from data for strain SX703, which exhibits only large transcriptional bursts, since it lacks both auxiliary operators. Choi et al. proposed that and represent the size and frequency of large transcriptional bursts. (b) Derived from raw data for strain SX701, which exhibits mostly small transcriptional bursts, since it has both auxiliary operators. Choi et al. did not consider this data on the grounds that the occurrence of large bursts in a few cells distorted the statistics of the small transcriptional bursts. (c) Derived from data for strain SX701 that was filtered by rejecting the data corresponding to the few cells exhibiting large bursts. Choi et al. proposed that this and represent the size and frequency of small transcriptional bursts. (d) Mean size of large transcriptional bursts in strain SX701, , (full red curve) and fraction of proteins derived from such bursts, , (full blue curve) estimated from the data in (b). The ordinate of the dashed red line is one-third of the ordinate of the vs. [TMG] line shown in (a), and therefore represents one-third of the (large) transcriptional burst size in strain SX703. The proximity of the full and dashed red lines implies that the mean size of large transcriptional bursts in strain SX701 is approximately one-third of the transcriptional burst size in strain SX703, which is consistent with our model predictions.</p

Burst frequency and size in uninduced cells with and without auxiliary operators.

<p> Calculated from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e270" target="_blank">eqs. (44)</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e273" target="_blank">(45)</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e280" target="_blank">(47)</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e281" target="_blank">(48)</a>, and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e299" target="_blank">(56)</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e300" target="_blank">(57)</a> with the parameter values in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-t001" target="_blank">Table 1</a>.</p><p> Determined from the data in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone-0102580-g002" target="_blank">Figs. 2</a> a–c by appealing to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e287" target="_blank">eqs. (53)</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e288" target="_blank">(54)</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e304" target="_blank">(61)</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102580#pone.0102580.e305" target="_blank">(62)</a>.</p

Functional annotation clusters in the up-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).

<p>Functional annotation clusters in the up-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).</p

Functional annotation clusters in the down-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).

<p>Functional annotation clusters in the down-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).</p

Activities of detoxification enzymes of resistant and susceptible lines.

<p>Activities of detoxification enzymes of resistant and susceptible lines.</p

Mapping of the lethality and resistance loci on the right arm of the second chromosome in the resistant line MiT[<i>w⁻</i>]3R2.

<p>The resistance locus was mapped relative to P element insertions to a region between 8 Mb and 8.5 Mb (black arrows on the second scale, distance between insertion and resistance region is indicated with dotted horizontal lines). The location of the three highly expressed P450 genes (Cyp6a2, Cyp6g1 and Cyp4p2) in the resistant MiT[<i>w⁻</i>]3R2 line is indicated. Below is a comparison of single nucleotide polymorphism (SNP) density (per 1 Kb) between resistant line MiT[<i>w⁻</i>]3R2 and susceptible line iso31. At the bottom, Bloomington deletions overlapping lethality locus (filled box) and flanking the lethality locus (open boxes) (lethality maps to the region between 8.5 Mb and 9.9 Mb, close to the place of recombination).</p

Crossing scheme of the genome-wide insertional mutagenesis system.

<p>TREP 2.30 – promoter delivery, minimal promoter under tTA control, <i>w⁺</i> marker <i>CyO</i> [MiT 2.4] – <i>Minos</i> transposase source, CyO marker <i>Sco</i> – Sco marker BOEtTA – tTA source, <i>egfp</i> marker.</p