Stereospecific Phosphorylation by the Central Mitotic Kinase Cdk1-Cyclin B

The <i>cis</i> vs <i>trans</i> conformation, or shape, of phosphoserine-proline (pSer-Pro), a prevalent motif in cell cycle proteins, may play a significant role in regulating mitosis. We demonstrate that Cdk1-cyclin B, the central mitotic kinase, is specific for the <i>trans</i> conformation, not <i>cis</i>, of synthetic, locked Ser-Pro 11-residue peptide substrates, using LC-MSMS detection and sequencing of phosphorylated products. This substrate stereospecificity may contribute an additional level of mitotic regulation

Additional file 1 of Spotlight on MuSK positive myasthenia gravis: clinical characteristics, treatment and outcomes

Additional file 1. [21, 22]

DataSheet1_A more novel and powerful prognostic gene signature of lung adenocarcinoma determined from the immune cell infiltration landscape.pdf

BackgroundLung adenocarcinoma (LUAD) is the leading histological subtype of lung cancer worldwide, causing high mortality each year. The tumor immune cell infiltration (ICI) is closely associated with clinical outcome with LUAD patients. The present study was designed to construct a gene signature based on the ICI of LUAD to predict prognosis.MethodsDownloaded the raw data of three cohorts of the TCGA-LUAD, GSE72094, and GSE68465 and treat them as training cohort, validation cohort one, and validation cohort two for this research. Unsupervised clustering detailed grouped LUAD cases of the training cohort based on the ICI profile. The univariate Cox regression and Kaplan–Meier was adopted to identify potential prognostic genes from the differentially expressed genes recognized from the ICI clusters. A risk score-based prognostic signature was subsequently developed using LASSO-penalized Cox regression analysis. The Kaplan-Meier analysis, Cox analysis, ROC, IAUC, and IBS were constructed to assess the ability to predict the prognosis and effects of clinical variables in another two independent validation cohorts. More innovatively, we searched similar papers in the most recent year and made comprehensive comparisons with ours. GSEA was used to discover the related signaling pathway. The immune relevant signature correlation identification and immune infiltrating analysis were used to evaluate the potential role of the signature for immunotherapy and recognize the critical immune cell that can influence the signature's prognosis capability.ResultsA signature composed of thirteen gene including ABCC2, CCR2, CERS4, CMAHP, DENND1C, ECT2, FKBP4, GJB3, GNG7, KRT6A, PCDH7, PLK1, and VEGFC, was identified as significantly associated with the prognosis in LUAD patients. The thirteen-gene signature exhibited independence in evaluating the prognosis of LUAD patients in our training and validation cohorts. Compared to our predecessors, our model has an advantage in predictive power. Nine well know immunotherapy targets, including TBX2, TNF, CTLA4, HAVCR2, GZMB, CD8A, PRF1, GZMA, and PDCD1 were recognized correlating with our signature. The mast cells were found to play vital parts in backing on the thirteen-gene signature's outcome predictive capacity.ConclusionsCollectively, the current study indicated a robust thirteen-gene signature that can accurately predict LUAD prognosis, which is superior to our predecessors in predictive ability. The immune relevant signatures, TBX2, TNF, CTLA4, HAVCR2, GZMB, CD8A, PRF1, GZMA, PDCD1, and mast cells infiltrating were found closely correlate with the thirteen-gene signature's power.</p

Supplementary document for Complex-Amplitude Modulation of Surface Waves Based on Metasurface Coupler - 6306887.pdf

efficiency analysis of the meta-device, diffraction-free feature and self-healing feature of the generated Airy bea

Polyamide Membranes with Tunable Surface Charge Induced by Dipole–Dipole Interaction for Selective Ion Separation

Nanofiltration (NF) has the potential to achieve precise ion–ion separation at the subnanometer scale, which is necessary for resource recovery and a circular water economy. Fabricating NF membranes for selective ion separation is highly desirable but represents a substantial technical challenge. Dipole–dipole interaction is a mechanism of intermolecular attractions between polar molecules with a dipole moment due to uneven charge distribution, but such an interaction has not been leveraged to tune membrane structure and selectivity. Herein, we propose a novel strategy to achieve tunable surface charge of polyamide membrane by introducing polar solvent with a large dipole moment during interfacial polymerization, in which the dipole–dipole interaction with acyl chloride groups of trimesoyl chloride (TMC) can successfully intervene in the amidation reaction to alter the density of surface carboxyl groups in the polyamide selective layer. As a result, the prepared positively charged (PEI-TMC)-NH2 and negatively charged (PEI-TMC)-COOH composite membranes, which show similarly high water permeance, demonstrate highly selective separations of cations and anions in engineering applications, respectively. Our findings, for the first time, confirm that solvent-induced dipole–dipole interactions are able to alter the charge type and density of polyamide membranes and achieve tunable surface charge for selective and efficient ion separation

MOESM1 of miR-145 sensitizes esophageal squamous cell carcinoma to cisplatin through directly inhibiting PI3K/AKT signaling pathway

Additional file 1: Fig. S1: Quantitative analysis of protein band gray in Fig. 3d

MOESM2 of miR-145 sensitizes esophageal squamous cell carcinoma to cisplatin through directly inhibiting PI3K/AKT signaling pathway

Additional file 2: Fig. S2: Quantitative analysis of protein band gray in Fig. 7c

Penetrated COF Channels: Amino Environment and Suitable Size for CO₂ Preferential Adsorption and Transport in Mixed Matrix Membranes

Developing mixed matrix membranes (MMMs) is challenging because the interface between different matrices often forms undesirable structures. Herein, we demonstrate a method of creating suitable CO2-selective channels based on interface regulation that greatly enhances membrane separation performance. The poly­(vinylamine), which also acts as a polymer matrix, was immobilized onto covalent organic frameworks (COFs) to obtain polymer–COF hybrid materials (COFp). The COFp and polymer matrix are highly compatible because they have the same segment. The polymer matrix was induced to penetrate the oversized COFp, resulting in an amino-environmental pore wall and appropriately sized CO2-selective channels dispersed in MMMs. The MMMs exhibited satisfactory membrane performance for CO2/N2, CO2/CH4, and CO2/H2 separation. A CO2 transport model for preferential adsorption and transport is clearly presented for the first time. The membrane separation mechanism is also discussed. This work demonstrates potential applications for material, interface, and membrane investigations

Positively Charged Polyamine Nanofiltration Membrane for Precise Ion–Ion Separation

Positively charged nanofiltration (NF) membranes offer enormous potential for lithium–magnesium separation, hard water softening, and heavy metal removal. However, fundamental performance limitations for these applications exist in conventional polyamide-based NF membranes due to the negatively charged surface and low ion–ion selectivity. We hereby innovatively develop an advanced positively charged polyamine-based NF membrane built by the nucleophilic substitution of bromine and amine groups for precise ion–ion separation. Specifically, polyethylenimine (PEI) and 1,3,5-tris(bromomethyl)benzene (TBB) are interfacially polymerized to generate an amine-linked PEI-TBB selective layer with an ultrathin thickness of ∼95 nm, an effective pore size of 6.5 Å, and a strong positively charged surface with a zeta potential of +20.9 mV at pH 7. The PEI-TBB composite membrane achieves a water permeance of 4.2 L·m–2·h–1·bar–1, various divalent salt rejections above 90%, and separation factors above 15 for NaCl/MgCl2 and LiCl/MgCl2 mixed solutions. A three-stage NF process is implemented to achieve a Mg2+/Li+ mass ratio sharply decreasing from 50 to 0.11 with a total separation factor (SLi,Mg) of 455. Furthermore, the polyamine-based NF membrane exhibits excellent operational stability under continuous filtration and high operational pressure, demonstrating great application potential for precise ion–ion separation

Tumor markers in milk and nipple discharge^*.

Tumor markers in milk and nipple discharge*.</p