11 research outputs found

    Overview of PCR-based and DNA sequencing results.

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    *<p>positivity criteria in analogy to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002163#pntd.0002163-Bustin1" target="_blank">[19]</a>. GenBank accession numbers:</p>1<p>BankIt1587796 Seq2 KC283067.</p>2<p>BankIt1587796 Seq3 KC283068.</p>3<p>BankIt1587796 Seq1 KC283066.</p

    Efficiency of dengue RNA extraction from RDT-S part (7mm), RDT-WS part (15 mm) and FP (2 disc of 6mm) compared to the direct extraction for all isolate dilutions for the four dengue serotypes.

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    <p>In y axis: RNA recovery in percentage: (RDTor FP/Dir)*100 = ratio of the mean number of dengue RNA copies/μl recovered by RDT(S part or WS: whole S part) or FP extraction over the mean number of dengue RNA copies/μl recovered by the direct extraction. X axis: RSD = relative standard deviation for the different extraction techniques.</p

    NS1 RDT strip cutting.

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    <p>A: NS1 cassette. B: strip outside the cassette with the location of the 4 parts cut for the experiment, N: nitrocellulose, C: conjugate pad, S: sample pad, N1 and N2: nitrocellulose membrane. C: strip with the location of the whole S pad (WS) cut for the experiment.</p