Additional file 2 of Impact of media compositions and culture systems on the immunophenotypes of patient-derived breast cancer cells

Supplementary Material

Additional file 3 of Impact of media compositions and culture systems on the immunophenotypes of patient-derived breast cancer cells

Supplementary Material

Additional file 1 of Impact of media compositions and culture systems on the immunophenotypes of patient-derived breast cancer cells

Supplementary Material

Cell-Penetrating Peptide-Patchy Deformable Polymeric Nanovehicles with Enhanced Cellular Uptake and Transdermal Delivery

We herein propose a polymeric nanovehicle system that has the ability to remarkably improve cellular uptake and transdermal delivery. Cell-penetrating peptide-patchy deformable polymeric nanovehicles were fabricated by tailored coassembly of amphiphilic poly­(ethylene oxide)-<i>block</i>-poly­(ε-caprolactone) (PEO-<i>b</i>-PCL), mannosylerythritol lipid (MEL), and YGRK­KRRQ­RRR-cysteamine (TAT)-linked MEL. Using X-ray diffraction, differential scanning calorimetry, and nuclear magnetic resonance analyses, we revealed that the incorporation of MEL having an asymmetric alkyl chain configuration was responsible for the deformable phase property of the vehicles. We also discovered that the nanovehicles were mutually attracted, exhibiting a gel-like fluid characteristic due to the dipole–dipole interaction between the hydroxyl group of MEL and the methoxy group of PEO-<i>b</i>-PCL. Coassembly of TAT-linked MEL with the deformable nanovehicles significantly enhanced cellular uptake due to macropinocytosis and caveolae-/lipid raft-mediated endocytosis. Furthermore, the <i>in vivo</i> skin penetration test revealed that our TAT-patchy deformable nanovehicles remarkably improved transdermal delivery efficiency