A New Porous Lithosilicate with a High Ionic Conductivity and Ion-Exchange Capacity

A New Porous Lithosilicate with a High Ionic Conductivity and Ion-Exchange Capacit

Additional file 2: of Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Materials and Methods. (DOCX 29 kb

A New Porous Lithosilicate with a High Ionic Conductivity and Ion-Exchange Capacity

A New Porous Lithosilicate with a High Ionic Conductivity and Ion-Exchange Capacit

Dehydrogenation Reaction Pathway of the LiBH₄–MgH₂ Composite under Various Pressure Conditions

This paper investigates dehydrogenation reaction behavior of the LiBH₄–MgH₂ composite at 450 °C under various hydrogen and argon back-pressure conditions. While the individual decompositions of LiBH₄ and MgH₂ simultaneously occur under 0.1 MPa H₂, the dehydrogenation of MgH₂ into Mg first takes place and subsequent reaction between LiBH₄ and Mg into LiH and MgB₂ after an incubation period under 0.5 MPa H₂. Under 1 MPa H₂, enhanced dehydrogenation kinetics for the same reaction pathway as that under 0.5 MPa H₂ is obtained without the incubation period. However, the dehydrogenation reaction is significantly suppressed under 2 MPa H₂. The formation of Li₂B₁₂H₁₂ as an intermediate product during dehydrogenation seems to be responsible for the incubation period. The degradation in hydrogen capacity during hydrogen sorption cycles is not prevented with the dehydrogenation under 1 MPa H₂, which effectively suppresses the formation of Li₂B₁₂H₁₂. The overall dehydrogenation behavior under argon pressure conditions is similar to that at hydrogen pressure conditions, except that under 2 MPa Ar

Additional file 4: Figure S2. of Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Competition enzyme immunoassay using polyclonal antibodies (pAbs) with the peptide YSATLRY. Sera from five volunteers showed reactivity to both YSATLRY and YSPTLFY, and pAbs were purified using gels cross-linked with YSATLRYGGGSC. To confirm pAb specificity, a competition enzyme immunoassay was performed. The microtiter plate wells were coated with BSA-conjugated YSATLRYGGGSC and blocked with 3 % BSA in PBS. A pre-mixture of pAbs and peptides was added to each well. After washing with 0.05 % PBST three times, plates were incubated with HRP-conjugated anti-human IgG antibodies. Washing steps were repeated three times. ABTS in 0.05 M citric acid buffer (pH 4.0) and 1.0 % H2O2 were added to each well. OD was measured at 405 nm with a microplate spectrophotometer. LPCYTDHICYSSGGGS was used as a control peptide. (DOCX 75 kb

Additional file 7: Figure S5. of Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Correlation of ANA antibody index with antibody reactivity to YSATLRY and YSPTLFY. The sera of 45 centenarian, 25 old, and 25 young volunteers were tested for ANA using an enzyme immunoassay kit. Optical densities were converted to antibody index according to the manufacturer’s instructions. (DOCX 48 kb

Additional file 2 of Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice

Supplementary Material

Additional file 3: Figure S1. of Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Enzyme immunoassay to determine antibody titer to YSATLRY (A) and YSPTLFY (B). Wells of a microtiter plate were coated with either YSATLRYGGGSC or YSPTLFYGGGSC peptide conjugated to BSA and blocked with 3 % BSA in PBS. Sera from individuals diluted in 3 % BSA in PBS were added to the wells. After washing with 0.05 % PBST three times, the plates were incubated with HRP-conjugated anti-human IgG antibodies. The washing steps were repeated three times. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) in 0.05 M citric acid buffer (pH 4.0) and 1.0 % H2O2 were added to each well. OD was measured at 405 nm with a microplate spectrophotometer. Asterisks indicate five selected volunteers for further studies (Volunteer #7 = pAb 7, Volunteer #11 = pAb 11, Volunteer #19 = pAb 19, Volunteer #47 = pAb 47, and Volunteer #50 = pAb 50). (DOCX 150 kb

Additional file 1: Table S1. of Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Age and gender distribution of subjects. (DOCX 18 kb

Image1_Millet seed oil activates β–catenin signaling and promotes hair growth.TIF

Alopecia, regardless of gender, exacerbates psychological stress in those affected. The rising prevalence of alopecia has fueled a research interest in preventing hair loss. This study investigates the potential of millet seed oil (MSO) in promoting the proliferation of hair follicle dermal papilla cells (HFDPC) and stimulating hair growth in animals with testosterone-dependent hair growth inhibition as part of a study on dietary treatments to improve hair growth. MSO-treated HFDPC significantly increased cell proliferation and phosphorylation of AKT, S6K1, and GSK3β proteins. This induces β-catenin, a downstream transcription factor, to translocate to the nucleus and increase the expression of factors related to cell growth. In a C57BL/6 mice model in which hair growth was inhibited by subcutaneous testosterone injection after shaving the dorsal skin, oral administration of MSO stimulated hair growth in the subject mice by increasing the size and number of hair follicles. These results suggest that MSO is a potent agent that may help prevent or treat androgenetic alopecia by promoting hair growth.</p