The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves

A series of experiments were conducted to evaluate the influence of supplemental dietary chromium (0 or 400 ppb) on insulin responsiveness in finishing steers (Exp 1. and Exp 2.), and energy status in newly weaned steers upon introduction to the feedlot (Exp 3.). To evaluate insulin responsiveness, two models were used with finished steers of different ages: Exp 1. 12 steers; 24 mo old (BW=783 ± 29 kg); and Exp 2. 24 steers; 14 mo old (BW=625 ± 24 kg). A jugular infusion of insulin (0.45 IU bovine insulin/ kg of BW0.75, INS) was pulse dosed 2 h post-prandial. A jugular infusion of PBS (SHAM) was included in Exp. 2. Blood samples were collected at -30, 120, 195, 255, and 315 min)\ relative to the morning feed delivery after 84 d (Exp 1.) and 86 d (Exp 2.) on test diets. No diet x time interactions were detected for any plasma metabolites in old or young steers. In Exp 2., INS effectively cleared plasma glucose (GLS). Diet had no impact on changes in GLS in Exp 2. Diet had no impact (P \u3e 0.20) on plasma concentrations of insulin in either experiment. In Exp 2., there tended (P=0.10) to be a diet x insulin interaction for post-infusion insulin levels. Insulin values were similar for all steers at (t195) and (t315) and elevated at (t255) for non-Cr fed steers subjected to INS. At t120, CrP caused greater (P \u3c 0.05) plasma urea-N (PUN) concentrations when compared to CON in Exp 1. but not in Exp 2. Effects of Cr on PUN in Exp 1. may be due to improved insulin sensitivity affecting AA uptake in the peripheral tissue of old, heavy steers. In Exp 3., there were no diet x day interactions (P\u3e0.20) for GLS, insulin, or PUN. The GLS and PUN levels were similar between diets (P \u3e 0.20). Insulin levels were higher (P0.20) between diets on d 5 and higher (P \u3c 0.05) on d 12 in calves fed CrP. The shift in NEFA on d 12 coincided with a spike in insulin levels. Both events occurred at the time that NEG intake was approaching the acclimated plateau and neither event impacted glucose status. The effective use of chromium in feedlot diets is likely dictated by age dependent differences in insulin sensitivity, metabolite needs for growth, and the degree of stress that ultimately result in a chromium deficient state

Evaluation of Dietary Roughage Inclusion in a Single or Two-Diet System for Backgrounding and Finishing

Study Description: Pre-conditioned crossbred beef steers (n = 46; initial shrunk [4%] BW = 621 ± 89.1 lbs) were used in a 210-d grow-finish experiment at the Ruminant Nutrition Center (RNC) in Brookings, SD. Steers were fed once daily, and bunks were managed according to a slick bunk management system. Cattle were fed in 25 × 25 ft concrete surface pens (n = 10 pens; 5 pens/treatment) with 25 linear ft of bunk space and heated, concrete, continuous flow waterers. Treatments included: 1) A single-diet program (targeted a 59 Mcal/cwt NEg diet fed for 210-d; 1D) or 2) two-diet program (targeted a 55 Mcal/cwt NEg diet fed for 98-d, a 59 Mcal/cwt NEg diet fed for 14-d, and a 63 Mcal/cwt NEg diet fed for 98-d; 2D). All steers were implanted initially (d 1) with a 100 mg trenbolone acetate (TBA) and 14 mg estradiol benzoate (EB) implant (Synovex Choice) and re-implanted with a 200 mg TBA and 28 mg EB implant on d 112

Impact of Corn Silage Moisture and/or Kernel Processing at Harvest on Finishing Steer Growth Performance, Efficiency of Dietary Net Energy Utilization and Carcass Traits

Study Description: Red Angus steers (n = 192; initial shrunk BW = 983 ± 62.3 lbs) were used in the 112 d finishing experiment at the Southeast Research Farm (SERF) of the South Dakota Agricultural Experiment Station in Beresford. Steers were from a single source and obtained from a local SD auction facility. Steers were received 2 weeks prior to trial initiation. Steers were offered a common diet containing 60% concentrate upon arrival. Steers were transitioned to a 90% concentrate diet over the course of 14 d. Steers were consuming the finishing diet (Table 1) at the initiation of the experiment. Fresh feed was manufactured once daily for each treatment in a single batch using a stationary mixer and bunks were managed for ad libitum access to feed. Actual diet formulation and composition is based upon weekly DM analyses, tabular nutrient values, and corresponding feed batching records. Diets presented in Table 1 are actual DM diet composition, tabular nutrient concentrations, and tabular energy values (Preston, 2016)

Access to Care, Reporting Behaviors, and Quality of Athletic Training Service Interactions for Reserve Officers\u27 Training Corps cadets

Purpose: Reserve Officers’ Training Corps (ROTC) programs prepare student-civilians to become leaders through strenuous physical and leadership training. Unlike their student-athlete counterparts who have direct access to athletic training services, ROTC cadets may or may not have a healthcare provider available. The purpose of this study was to examine the access to care and reporting behaviors of ROTC cadets with a secondary aim exploring the quality of healthcare service interactions relative to patient-centered care. Methods: An online survey assessed access to care using a self-report tool on the type of medical providers available to the ROTC cadets (n=132, age=20±3 y) dispersed between the Army, Navy, Air Force, and Marines, and their illness/injury history and reporting behaviors. The participants who sought care for an injury/illness also completed the Consultation and Relational Empathy tool to measure the level of patient-centered care by the healthcare provider with follow-up analysis using the Consultation Care Measure tool for all athletic training service interactions. Data were analyzed using descriptive statistics. Results: ROTC cadets reported access to 2±1 healthcare providers including a designated civilian physician (26.5%), athletic trainer (23.5%), and ROTC peer first responder (14.4%). However, 50.8% of respondents stated they were unsure what healthcare providers were available. In total, 22.7% of cadets reported being injured and 26.5% reported being sick/ill while participating in ROTC activities. Of those who stated they had sustained an injury during ROTC, 59.9% seldomly or never reported their injury. The ROTC cadets who sought healthcare expressed they were satisfied with their injury (35.96±10.60) and illness (35.48±13.10) treatment from a patient-centered viewpoint. Conclusions: The ROTC cadets reported a general unfamiliarity with the healthcare providers available to them. Despite the reporting behaviors, the cadets reported being satisfied with the care they received

Evaluation of a Direct Fed Microbial an an Enzymatically Hydrolyzed Yeast Product Fed Alone or in Combination to Beef Steers Administered Ractopamine Hydrochloride 28 Days Prior to Harvest During Summer Months in the Northern Plains

Study Description: Single-sourced, newly weaned steers (n=256; initial BW=542 ± 3.7lb; n=64 steers/treatment; 8 steers/pen) were blocked by location in a 2×2 factorial arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Temperature-humidity index (THI) was calculated as: THI=0.81×ambient temperature+[relative humidity×(ambient temperature-14.40)]+46.40. On d-1 and 2 and d-21 and 22 on RH, respiration rate (RR) and panting scores (PS) were determined before and after AM and PM feedings (0700h, 1100h, 1400h, 1700h). RR (n=3 steers/pen) was calculated from: 600/seconds required for 10 flank movements. PS utilized this scoring system: 0 (not distressed) to 4.5 (severely distressed)

Evaluation of a Direct Fed Microbial and/or an Enzymatically Hydrolyzed Yeast Product in Diets Containing Monensin Sodium on Feedlot Phase Growth Performance, Efficiency of Dietary Net Energy Utilization, and Carcass Characteristics in Newly Weaned Beef Steers Fed in Confinement for 258 Days

Study Description: Single-sourced, newly weaned steers (n = 256; initial body weight (BW) = 542 ± 3.7 lb) were allotted to 32 pens (n = 8 pens/treatment with 8 steers/pen). Steers were blocked by location in a 2x2 factorial treatment arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Steers were vaccinated and poured at processing and individually weighed on d 1, 14, 42 (end of receiving phase; implanted), 77, 105 (end of growing phase), 133, 161 (implanted), 182, 230 (start ractopamine HCl) and 258. Growth performance and carcass measurements were recorded

Anterior Cervical Infection: Presentation and Incidence of an Uncommon Postoperative Complication.

STUDY DESIGN: Retrospective multi-institutional case series. OBJECTIVE: The anterior cervical discectomy and fusion (ACDF) affords the surgeon the flexibility to treat a variety of cervical pathologies, with the majority being for degenerative and traumatic indications. Limited data in the literature describe the presentation and true incidence of postoperative surgical site infections. METHODS: A retrospective multicenter case series study was conducted involving 21 high-volume surgical centers from the AOSpine North America Clinical Research Network, selected for their excellence in spine care and clinical research infrastructure and experience. Medical records for 17 625 patients who received cervical spine surgery (levels from C2 to C7) between January 1, 2005, and December 31, 2011, inclusive, were reviewed to identify the occurrence of 21 predefined treatment complications. Patients who underwent an ACDF were identified in the database and reviewed for the occurrence of postoperative anterior cervical infections. RESULTS: A total of 8887 patients were identified from a retrospective database analysis of 21 centers providing data for postoperative anterior cervical infections (17/21, 81% response rate). A total of 6 postoperative infections after ACDF were identified for a mean rate of 0.07% (range 0% to 0.39%). The mean age of patients identified was 57.5 (SD = 11.6, 66.7% female). The mean body mass index was 22.02. Of the total infections, half were smokers (n = 3). Two patients presented with myelopathy, and 3 patients presented with radiculopathic-type complaints. The mean length of stay was 4.7 days. All patients were treated aggressively with surgery for management of this complication, with improvement in all patients. There were no mortalities. CONCLUSION: The incidence of postoperative infection in ACDF is exceedingly low. The management has historically been urgent irrigation and debridement of the surgical site. However, due to the rarity of this occurrence, guidance for management is limited to retrospective series

Dose Effects of Encapsulated Butyric Acid and Zinc on Beef Feedlot Steer Growth Performance, Carcass Characteristics, and Dietary Net Energy Utilization

Study Description: Steers (n = 272; shrunk BW = 794 ± 163 pounds) were assigned to one of four dietary treatments in a 143.5 d feedlot finishing trial: 0 g BPZ/ kg diet dry matter (DM) (CON), 1 g BPZ/ kg diet DM (1BPZ), 2 g BPZ/ kg diet DM (2BPZ), or 3 g BPZ/ kg diet DM (3BPZ). Carcass data and liver health outcomes were collected, and feedlot growth performance data and efficiency of dietary net energy utilization were calculated on a carcass-adjusted basis

Effects of On-Arrival Application of a Modified-Live Respiratory and Clostridia Vaccination on Health, Growth Performance, and Antibody Titers of Newly-Weaned Calves

Study Description: Single-sourced, newly weaned steers (n=70; initial body weight (BW)=560±12.9lb) were allotted to 10 pens (n=5 pens/treatment with 7 steers/pen). Steers were blocked by BW in a randomized complete block design of VAC [vaccinated for IBR, BVD 1 and 2, PI3, and BRSV (Bovi-Shield Gold 5, Zoetis, Parsippany, NJ) and clostridial (Ultrabec 7/Somubac, Zoetis) upon arrival] or NOVAC (not vaccinated for IBR, BVD 1 and 2, PI3, and BRSV or clostridial species upon arrival). Steers were individually weighed on d 0 (arrival), 1, 21, and 42 for growth performance measures. Whole blood samples (10 mL) were collected (n=3 steers/pen closest to the pen mean BW) on d 1, 21, and 42 via jugular venipuncture for metabolite and antibody titer responses

Effects of Premortem Stress on Protein Expression, Steak Color, Oxidation, And Myofibrillar Fragmentation Index in the \u3ci\u3eLongissimus Lumborum\u3c/i\u3e

Forty castrated Holstein calves underwent an adrenocorticotropic hormone (ACTH) challenge to assess the effects of premortem stress on the longissimus lumborum (LL) following harvest. LL biopsies were collected before the challenge, at different harvest times (2, 12, 24, and 48 h; n = 10), and after 14 d aging. The expression of small heat shock proteins (SHSPs), deglycase 1 (DJ-1), and troponin were analyzed. Blood was analyzed throughout the ACTH challenge and at harvest for cortisol, oxidative stress, and complete blood count (CBC). Color and myofibrillar fragmentation index (MFI) were measured in aged samples. Unexpectedly, calves from different harvest times differed (p = 0.05) in cortisol response. Calves were divided into two different cortisol response groups (high or low; n = 20). Statistical analysis assessed the effects of cortisol response (n = 20), harvest time (n = 10), and their interaction. Harvest time altered SHSPs (p = 0.03), DJ-1 (p = 0.002), and troponin (p = 0.02) expression. Harvest time and cortisol response impacted steak color (p < 0.05), and harvest time altered steak pH (p < 0.0001). Additionally, various CBCs were changed (p < 0.05) by harvest time. Harvest time changed (p = 0.02) MFI. These data demonstrate that the protein expression, color, and MFI of the LL may be influenced by premortem stress