Antimicrobial Activity of Liquid Soap Containing Turmeric (Curcuma domestica val.) and Lemongrass (cymbopogon nardus l.) Leaf Juice

Abstract. The purpose of this study was to formulate an antimicrobial liquid soap containing 25% turmeric and lemongrass leaf juice. Lemongrass leaf juice and turmeric juice 25%  were formulated into a liquid soap. Stability, pH and viscosity of the liquid soap were evaluated. For stability tests, the preparation was stored for 3 months at room temperaure. The pH test was measured by Hanna pH meter. The viscosity test,was measured by Brookfield viscometer.The antimicrobial activity of the liquid soap was measured by disc diffusion method using bacteria such as Staphylococcus epidermis, Eschericia coli, Microsporum gypseum and Candida albicans. Physical properties of the formulated liquid soap including pH, viscosity, and stability showed that it was stable for 3 months. The results of antimicrobial test of the liquid soap containing turmeric and lemongrass leaf juice showed that the inhibition diameter against Staphylococcus epidermis, Eschericia coli, Microsporum gypseum and Candida albicans were 22.89, 27.05, 24.30, and 33.05 mm, respectively, while the liquid soap without the juice were 20.76, 19.50, 20.50 and 21.46 mm, respectively. The soap was effective in inhibiting the growth of microbes. Combination of turmeric and lemongrass leaf juice increased the inhibitory zone diameter of the liquid soap for microbe.  Keywords: Antimicrobial Agent, Lemongrass Leaf, Liquid Soap, Turmeric Leaf

PERBANDINGAN UJI AKTIVITAS ANTIDIABETES EKSTRAK DAUN LIDAH BUAYA DAN LIDAH MERTUA PADA MENCIT PUTIH JANTAN

Diabetes mellitus (DM) is a disease whose prevalence is increasing from year to year. This increase was accompanied by an increase in the cost of treatment and the number of complications. The use of traditional medicine is an alternative for the community to control blood sugar levels (KGD). Considering that synthetic drugs have various side effects, one of the medicinal plants used as traditional medicine is aloe vera leaf and mother-in-law's tongue leaf which contain flavonoid compounds, alkaloids, tannins, saponins, and steroids/triterpenoids. The purpose of this study was to determine the antidiabetic activity of aloe vera leaf extract and mother-in-law's tongue and to compare the activities of the two. The results of the analysis of the glucose tolerance ANOVA test showed a significant difference in the percentage reduction at 60, 90, and 120 minutes for all treatment preparations. The alloxan induction test showed a significant difference in the percentage decrease from days 3, 5, 7, 9, 11, 13, and 15. Based on these results, it can be concluded that Aloe Vera Leaf Ethanol Extract (EEDLB) and LidahMertua Leaf Ethanol Extract (EEDLM) with each dose of 150 and 200 mg/kg BW and the combination of the two gave antidiabetic activity against mice as well as differences in effectiveness between samples of aloe vera leaf and LidahMertua leaf as well as a single dose with a combination

Antibacterial Activity of Ethyl Acetate Fraction of Passion Fruit Peel (Passiflora Edulis Sims) on Staphylococcus Aureus and Escherichia Coli

North Sumatera is one of the central areas of purple passion fruit production in Indonesia. Processing passion fruit into beverage products (passion fruit juice) produces peel  has not been utilized. The use of passion fruit skin needs to be studied so that it can be useful as a raw material for antibacterial drug preparations. The aim of this study was to determine the phytochemical constituent screening  and antibacterial activity of ethyl acetate fraction of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. Simplicia and ethyl acetate fraction were determinated  its phytochemical properties.  The extraction process by percolation method using ethanol  96% and continue to fractionation process by liquid liquid extraction method using n-hexane and ethyl acetate. The antibacterial activity were tested  against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs. The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethyl acetate fraction showed the presence of flavonoids, glycosides, saponins and tannins. The antibacterial activity test showed that the ethyl acetate fraction has an effective inhibition at the concentration of 100 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner. The ethyl acetate fraction of passion fruit peel (Passiflora edulis Sims) has an antibacterial activity  on Staphylococcus aureus and Escherichia col

Antibacterial activity of Ethanol Extract of Purple Passion Fruit Peel (Passiflora edulis Sims) on Staphylococcus aureus and Escherichia coli

Abstract.North Sumatera is the central production of  purple passion fruit  in Indonesia. Passion fruit was  processing into beverage product that produced passion fruit peel waste. The passion fruit peel has potential effect as a sources of medicine which is need to be examined such as its antibacterial effect. The purpose of this study was to determine the characterization of simplicia, phytochemical screening  and antibacterial activity of ethanol extract of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. The simplicia powder was examined its characteristic include water content, water soluble extract, ethanol soluble extract, total ash and acid insoluble ash assay. The extraction was process by percolation method using ethanol  96% , then tested antibacterial activity against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs.The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethanol extract showed the presence of flavonoid, glycoside, saponin, tannin and steroid/triterpenoid.The antibacterial activity test showed that the ethanol extract has an effective inhibition at the  concentration of 300 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner.   Keywords: Passiflora edulis Sims, antibacterial, passion fruit peel, Staphylococcus aureus and Escherichia coli       &nbsp

PENETAPAN KADAR PROTEIN DALAM SUSU FORMULA BAYI DAN ASI DI MEDAN SECARA KJELDAHL

Breast milk (ASI) is the best food for babies. Breast milk has advantages compared to other milk, including guaranteed cleanliness, the composition of breast milk is also very suitable for digestion, growth, and development of babies, one of the compositions contained in breast milk is protein. Currently, many mothers cannot breastfeed their own babies, so they choose infant formula as a substitute for breast milk. The purpose of this study was to determine differences in protein levels in infant formula and breast milk. Determination of protein content was carried out using the Kjeldahl method which is a simple method for determining total nitrogen in protein and other nitrogen-containing compounds. The Kjeldahl method is carried out through 3 processes, namely: the destruction process, the distillation process, and the titration process according to the procedures and tools used in the Chemistry Laboratory of the Sari Mutiara Indonesia University. The results of the determination of protein levels using the Kjeldahl method contained a protein with average levels in infant formula brand A (10.10 -+ 0.96) %, infant formula brand B (10.95 -+ 2.43) %, infant formula brand C (11.0666 -+ 1.6932) %, and breast milk (20.5733 -+ 3.1828) %

PENETAPAN KADAR PROTEIN DALAM SUSU FORMULA BAYI DAN ASI DI MEDAN SECARA KJELDAHL

Breast milk (ASI) is the best food for babies. Breast milk has advantages compared to other milk, including guaranteed cleanliness, the composition of breast milk is also very suitable for digestion, growth, and development of babies, one of the compositions contained in breast milk is protein. Currently, many mothers cannot breastfeed their own babies, so they choose infant formula as a substitute for breast milk. The purpose of this study was to determine differences in protein levels in infant formula and breast milk. Determination of protein content was carried out using the Kjeldahl method which is a simple method for determining total nitrogen in protein and other nitrogen-containing compounds. The Kjeldahl method is carried out through 3 processes, namely: the destruction process, the distillation process, and the titration process according to the procedures and tools used in the Chemistry Laboratory of the Sari Mutiara Indonesia University. The results of the determination of protein levels using the Kjeldahl method contained a protein with average levels in infant formula brand A (10.10 -+ 0.96) %, infant formula brand B (10.95 -+ 2.43) %, infant formula brand C (11.0666 -+ 1.6932) %, and breast milk (20.5733 -+ 3.1828) %

PENETAPAN KADAR PROTEIN DALAM SUSU FORMULA BAYI DAN ASI DI MEDAN SECARA KJELDAHL

Breast milk (ASI) is the best food for babies. Breast milk has advantages compared to other milk, including guaranteed cleanliness, the composition of breast milk is also very suitable for digestion, growth, and development of babies, one of the compositions contained in breast milk is protein. Currently, many mothers cannot breastfeed their own babies, so they choose infant formula as a substitute for breast milk. The purpose of this study was to determine differences in protein levels in infant formula and breast milk. Determination of protein content was carried out using the Kjeldahl method which is a simple method for determining total nitrogen in protein and other nitrogen-containing compounds. The Kjeldahl method is carried out through 3 processes, namely: the destruction process, the distillation process, and the titration process according to the procedures and tools used in the Chemistry Laboratory of the Sari Mutiara Indonesia University. The results of the determination of protein levels using the Kjeldahl method contained a protein with average levels in infant formula brand A (10.10 ± 0.96) %, infant formula brand B (10.95 ± 2.43) %, infant formula brand C (11.0666 ± 1.6932) %, and breast milk (20.5733 ± 3.1828) %

The Effect of Turmeric Extract (Curcuma Domestica Val) Against the Durability of Yellow Rice Storage

Yellow cooked rice is a traditional food in some regions in Indonesia. Yellow cooked rice with the addition of turmeric and flavoring which can longer shelf life of savings because it has antibacterial activity and flavor. The purpose of this study was to determine the effect of turmeric extract (Curcuma domestica Val) to extend shelf life of yellow cooked rice. The samples used were made of yellow cooked rice with handmade and without any seasoning flavored with turmeric extract concentration of 1.8% and 2.4%. Organoleptic testing were conducted every 3 hours, test of microbial growth daily and inhibition of turmeric extract with a concentration of 1.8% and 2.4% of fungi isolated from yellow cooked rice with a total plate count method. Research showed that turmeric extract concentration and the addition of  herbs affect the longer shelf life of yellow cooked rice store. High more and more  concentration of the turmeric extract can made longer the shelf life of yellow cooked rice either through organoleptic and also total plate count. Yellow cooked rice without seasoning with turmeric extract concentration of 1.8% and 2.4% were 30 and 33 hours respectively, while the yellow cooked rice was flavored with the same concentration of turmeric extract  were 36 and 39 hours respectively

PEMERIKSAAN Escherichia coli PADA DEPOT AIR MINUM ISI ULANG DIKECAMATAN KOTA KUALA SIMPANG

The availability of clean water is decreasing along with the development of dense population growth. Many households choose Refill Drinking Water as an alternative because the price is relatively cheap. However, no one can guarantee the pathogenic bacteria contained in Refill Drinking Water. This study aims to determine the presence of Escherichia coli bacteria in refill drinking water in Kualasimpang City. This type of research is descriptive with an observational approach. The population in this study were all Refill Drinking Water Depots (DAMIU) in Kualasimpang City in 2018, totaling 21 Refill Drinking Water Depots (DAMIU). The research sample was distributed gallon water, this sample was taken from 5 Refill Drinking Depots which were selected using the Random Sampling method. The results of the study did not show the presence of Escherichia coli bacteria in refilled drinking water but only Coliform pathogenic bacteria. The quality of the water means that it is in accordance with the standards of the Minister of Health of the Republic of Indonesia No.492/MENKES/PER/IV/2010 concerning the requirements for the quality of drinking water, where drinking water must not contain bacteria in it