Primer details to sequence the coding sequence of AKT1.

<p>Primers in 5′ to 3′ direction.</p

Characteristics of 109 GBM tumor samples.

<p>Characteristics of 109 GBM tumor samples.</p

Additional file 1 of Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancer

Additional file 1. Supplementary figures S1-S9

PCR primers used for the mutational profiling.

<p>PCR primers used for the mutational profiling.</p

Sequence homology (at the protein level) and expression distribution in human tissues among Gq family genes.

<p>Sequence homology (at the protein level) and expression distribution in human tissues among Gq family genes.</p

Number of samples analyzed for each histological type and the number of mutations identified.

<p><b>(AML</b>: acute myeloid leukemia, <b>GIST</b>: Gastrointestinal Stromal Tumors; In addition, p-values of the Fisher's exact test, used to determine the tissue specificity for <i>GNAQ</i>^Q209 mutations in blue naevi, are listed.)</p

Example of one of the <i>GNAQ</i> mutations identified in blue naevi.

<p>The arrow indicates the location of the missense change p.Q209L (c.A627T).</p

Following evaluation of <i>KRAS</i> status in individual tumors, enhancement of predictability of clinical benefit may derive from assessment of the status of <i>BRAF</i>, <i>PIK3CA</i> and PTEN, as simulated here based on analyses of subgroups from the present cohort (n = 131).

<p>We propose to define as “quadruple negative” the mCRCs lacking alterations in <i>KRAS</i>, <i>BRAF</i>, PTEN and <i>PIK3CA</i>.</p

Supplementary Material from BCAM and LAMA5 Mediate the Recognition between Tumor Cells and the Endothelium in the Metastatic Spreading of KRAS-Mutant Colorectal Cancer

The supplementary material includes Supplementary Methods, two Supplementary Tables, and six Supplementary Figures Table S1. Complete set of BCAM-mimic peptides. Table S2. Characterization of human CRC cell lines in animal models. Figure S1. Quality control of the phage display experiments: sequencing readout. Figure S2. BCAM protein expression in the complete panel of human hepatic metastases. Figure S3. BCAM protein expression in human primary CRCs. Figure S4. BCAM protein and mRNA expression in all cell lines. Figure S5. Photographic documentation of the experimental hepatic metastasis models. Figure S6. HT-55, DLD-1, LIM1215 and SW-48 cells cannot be reduced to adhesion-functional monocellular suspensions.</p

Multivariate analysis of survival done with exact logistic regression among <i>KRAS</i> wild-type patients.

<p>Hazard ratio values are adjusted by score of cutaneous toxicity and number of previous chemotherapy lines.</p><p>PFS: progression-free survival; OS: overall survival.</p