Facile Low Temperature Hydrothermal Synthesis of Magnetic Mesoporous Carbon Nanocomposite for Adsorption Removal of Ciprofloxacin Antibiotics

A new magnetic mesoporous carbon composite (Fe₃O₄/C) was synthesized and characterized by XRD, nitrogen adsorption–desorption isotherms, FT-IR, TG, ζ potential, SEM, and TEM. The performance of using Fe₃O₄/C composite as an adsorbent for removal of antibiotics using ciprofloxacin (CIP) as a model was investigated. The Langmuir adsorption isotherm was applicable to fit the removal process. Kinetics of the CIP removal was found to follow a pseudo-second-order rate equation. The solution pH is critical for the adsorption of CIP on Fe₃O₄/C, and the maximum adsorption of CIP could be reached under neutral conditions. In addition, the Fe₃O₄/C adsorbent showed high magnetization and operational stability, and it could be readily separated from solution by applying an external magnetic field. After 10 recycle runs, over 85% of the adsorption capacity was retained. The high performance, low cost, and easily recyclable Fe₃O₄/C composite may become a promising adsorbent for water treatment

Additional file 1 of NUSAP1 promotes pancreatic ductal adenocarcinoma progression by drives the epithelial-mesenchymal transition and reduces AMPK phosphorylation

Additional file 1: Supplementary Information files. Original versions of Western blots in Fig. 6c, Fig. 8b, Fig. 10a, and Fig. 10b

TGFB1-induced autophagy affects the pattern of pancreatic cancer progression in distinct ways depending on SMAD4 status

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies. Given that macroautophagy/autophagy activation is prevalent in PDAC, the dual roles of autophagy could be involved in PDAC heterogeneity. In this work, we demonstrated that TGFB1 induced autophagic flux through SMAD4-dependent or SMAD4-independent pathways based on a distinct genetic context. In SMAD4-positive PDAC cells, TGFB1-induced autophagy promoted proliferation and inhibited migration by decreasing the nuclear translocation of SMAD4. Conversely, TGFB1-induced autophagy inhibited proliferation and promoted migration in SMAD4-negative cells through the regulation of MAPK/ERK activation. TGFB1 expression also positively correlated with LC3B expression in PDAC specimens. A high level of LC3B was associated with unfavorable overall survival (OS) and disease-free survival (DFS) in SMAD4-negative PDAC patients, although LC3B could not predict OS and DFS for the 110 PDAC patients. Thus, TGFB1-induced autophagy contributed to the different patterns of PDAC progression. This knowledge can aid in improving our understanding of the molecular classification of PDAC and might guide the development of therapeutic strategies for PDAC, especially for SMAD4-negative PDAC. Abbreviations: CDH1: cadherin 1; CDH2: cadherin 2; CI: combination index; CQ: chloroquine; DFS: disease-free survival; EMT: epithelial-to-mesenchymal transition; ERK: extracellular signal-regulated protein kinase; GFP: green fluorescent protein; IHC: immunohistochemistry; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAPK: mitogen-activated protein kinase; OS: overall survival; PBS: phosphate-buffered saline; PDAC: pancreatic ductal adenocarcinoma; RAP: rapamycin; RFP: red fluorescent protein; RT: room temperature; shRNA: short-hairpin RNA; SQSTM1: sequestosome 1; TCGA: The Cancer Genome Atlas; TEM: transmission electron microscopy; TGFB1: transforming growth factor beta 1; TMA: tissue microarray</p

Additional file 1: of A PD-L2-based immune marker signature helps to predict survival in resected pancreatic ductal adenocarcinoma

Table S1. Stromal PD-L2 expression and cell densities of TILs in relation to clinicopathologic characteristics of PDAC. Table S2. Clinicopathologic characteristics of PD-L2 expression and cell densities of TILs of PDAC in validation set. Table S3. Univariate and multivariate analysis of overall survival factors in validation set. Table S4. The correlation of TGF-β2 expression and immune markers in PDAC using 2-tailed χ2 test. Fig. S1. PD-L1 expression in PDAC. (PDF 877 kb

Additional file 3 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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Additional file 1 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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Additional file 2 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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Additional file 6 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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Additional file 4 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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Additional file 5 of Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

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