DataSheet1_Comparative efficacy and safety of Chinese medicine injections combined with capecitabine and oxaliplatin chemotherapies in treatment of colorectal cancer: A bayesian network meta-analysis.PDF

Objective: The aim of the present Bayesian network meta-analysis (NMA) was to explore the comparative effectiveness and safeaty of different Chinese Medicine injections (CMIs) combined with the XELOX regimen versus XELOX alone for colorectal cancer (CRC).Methods: A comprehensive search for randomized controlled trials (RCTs) was performed with regard to different CMIs for the treatment of CRC in several electronic databases up to April 2022. The quality assessment of the included RCTs was conducted according to the Cochrane risk of bias tool. Standard pair-wise and Bayesian NMA were designed to comparethe effectiveness and safety of different CMIs combined with the XELOX regimen by utilizing R 4.0.3 software and Stata 15.1 software simultaneously.Results: Initially, a total of 4296 citations were retrieved through comprehensive searching, and 32 eligible articles involving 2847 participants and 11 CMIs were ultimately included. CMIs combined with XELOX were superior to the XELOX regimen alone, and a total of ten Observation Indicators were included in the study, with the following results. Among all the injections, Shengmaiyin, Shenmai, and Kanglaite combined with the XELOX regimen were the three CMIs with the highest clinical efficiency. The top three in terms of improving CD3+ values were Shengmaiyin, Shenqifuzheng, and Cinobufacini injections. Shenqifuzheng, Shengmaiyin, and BruceaJavanica oil injections combined with the XELOX regimen performed best at raising CD4+ values. Kanglaite, Cinobufacini, and Matrine injections combined with the XELOX regimen performed best in improving CD4+/CD8+ rates. The top three in terms of improving performance status were Xiaoaiping, Shenmai, and Kanglaite injections. Cinobufacini and Brucea Javanica oil injections combined with the XELOX regimen performed best at raising CD8+ values. Shenqifuzheng, Kangai, and Matrine injections combined with the XELOX regimen performed best in improving Gastrointestinal reactions.The top threein terms of improving Leukopenia were Shenqifuzheng, Compound Kushen and Kanglaite injections. The top three in terms of improving Platelet decline were Compound Kushen, Cinobufacini and Shenqifuzheng injections. Additionally, those that were best at improving nausea and vomitting were Cinobufacini, Compound Kushen and Aidi injections.Conclusion: The results of the analysis demonstrated thatShengmaiyin, Kanglaite, and Cinobufacini injections and the XELOX regimen were associated with morepreferable and beneficial outcomes than other CMI groups. Nevertheless, additional results from multicenter trials and high-quality studies will bevital to support our findings.Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=326097, CRD42022326097.</p

Surface Preparation for Single-Molecule Fluorescence Imaging in Organic Solvents

The development of single-molecule techniques provides opportunities to investigate the properties and heterogeneities of individual molecules, which are almost impossible to be obtained in ensemble measurements. Recently, single-molecule fluorescence microscopy is being applied more and more to study chemical reactions in organic solvents. However, little has been done to optimize the surface preparation procedures for single-molecule fluorescence imaging in organic solvents. In this work, we developed a method to prepare the surface for single-molecule fluorescence imaging in organic solvents with a well-controlled surface density of chemically immobilized dye molecules and a low density of nonspecifically adsorbed impurities. We also compared the surfaces prepared by two different procedures and studied the impacts of the polarities of the solvent and the surface functionality on the quality of prepared surface. We found that higher polarities of both the solvent and the surface functionality provided better control of the surface density of chemically immobilized dyes and helped reduce the nonspecific adsorption of both dyes and fluorescent impurities in organic solvents. We further performed single-molecule fluorescence imaging in DMF and investigated the photophysical properties of dyes and fluorescent impurities, which could be used to filter out false counts in single-molecule fluorescence measurements

EBER sequence variations in 12 EBVaGC in GRC and 3EBVaGC in RGC.

<p>Names in the left column refer to EBER variants identified in this study. Names in the near column refer to the representative isolates, and the numbers in the parentheses after the name denote the number of isolates carried identical sequence with the representative isolate. An asterisk indicates a deletion of a nucleotide. EB-6m was the only variant in the present study. The initial specimen identified as EBVaGC was also being analysed.</p

Diagnostic significance of microRNA-1255b-5p in prostate cancer patients and its effect on cancer cell function

Discerning between indolent and aggressive types is a big challenge of prostate cancer clinically to guide the adequate therapeutic regimen. We aimed to examine the relationship between miR-1255b-p expression and prostate cancer and elucidate the function of miR-1255b-5p in prostate cancer. miR-1255b-5p were measured using Quantitative Real-Time PCR from the blood 103 benign prostate hyperplasia (BPH) and 153 prostate cancer patients (117 indolent cases and 36 upgrading cases). Using receiver operating characteristic (ROC) curve analysis, the discriminating ability of miR-1255b-5p was accessed between BPH and prostate cancer participants, or indolent and aggressive type. Using CCK-8 and Transwell assays, the function of miR-1255b-5p on prostate cancer cells was investigated. The levels of miR-1255b-5p were significantly raised in prostate cancer patients when compared with BPH participants. MiR-1255b-5p level can distinguish prostate cancer patients from BPH or indolent type from aggressive type. Downregulation of miR-1255b-5p can suppress the proliferative, invasive, and migratory capacity, but this effect can be eradicated by EPB41L1 inhibition. The measurement of miR-1255b-5p in blood may provide a new noninvasive approach for the diagnosis of prostate cancer. miR-1255b-5p may become a potential therapeutic target for prostate cancer.</p

PCR analysis for EBV genotyping for 1/2,F/f,C/D.

<p><b>Lanes 1–8:</b> Lane M, DL 2000 DNA Marker; lane 1, negative control; lane 2, B95-8 prototype (Type 1); lane 5,7,8, representative cases of Type 1; lane 3,4,6,representative case of co-infection with Type 1 and 2; no subtype 2 was detected in the study. <b>Lanes 9–16:RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI W1/I1 region.</b> Lane M, DL2000 DNA Marker; lane 9, negative control; lane 10, Raji prototype (Type D); lane 11, 13 and 15, representative cases of Type C strains; lane 12,14,16, representative cases of Type D strains. <b>Lanes 17–24: RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI F region.</b> Lane M, DL2000 DNA Marker; lane 17, negative control; lane 18, B95-8 prototype (Type F); lane 19–24 representative cases of the Type F strains; No type f strain was found in the present study.</p

PCR analysis for 30bp deletion in LMP1.

<p>Lane M, DL2000 DNA Marker; lane 1, negative control; lane 2 and lane 8, representative cases of wild type of LMP1; lane 3,4,5,6,7 representative cases of del-LMP1.</p

EBNA1 sequence variations in EBVaGC.

<p>Numbers across the top correspond to the amino acid positions under which the B95-8 prototype amino acid and nucleotide sequence is listed. Different patterns are noted to the left column, while the specimens showing identical sequences to each other are listed by a representative isolate in the second column. The followed numbers in the parentheses denote the amount of the identical sequences from EBVaGC. Four EBVaGC specimen from GRC were not amplified successfully. V-val was the only variant in all EBVaGC cases. The initial specimen identified as EBVaGC was also being analysed.</p

LMP1 encoded by Epstein–Barr virus mayactivate AHR through the ERK pathway innasopharyngeal carcinoma cells: supplementary files

Aim: To investigate the role of AHR in nasopharyngeal carcinoma (NPC) and explore the relationshipbetween Epstein–Barr virus (EBV) infection and the AHR pathway. Methods: The effect of LMP1 on theexpression of AHR was analyzed using real-time PCR and western blot. Proliferation and migration of cellswere assessed using CCK8 and Transwell analysis. Results: EBV infection downregulated the expressionof AHR in NPC cells, possibly through activation of the ERK pathway by LMP1 thereby acceleratingAHR proteasomal degradation following translocation to the nucleus. Cell proliferation, migration andautophagy are promoted by activating the AHR pathway. Conclusion: In NPC cells, LMP1 increases thephosphorylation of ERK, which may activate the AHR pathway.</p

Microscale Mn₂O₃ Hollow Structures: Sphere, Cube, Ellipsoid, Dumbbell, and Their Phenol Adsorption Properties

Various Mn2O3 hollow structures, such as spheres, cubes, ellipsoids, and dumbbells have been synthesized through the following process: The surfaces of the prepared MnCO3 microspheres, microcubes, and microellipsoids were oxidized by KMnO4 to form a core/shell structure. Similarly, the surface of a dumbbell-like MnCO3 intermediate can also be oxidized by KMnO4. As the MnCO3 or MnCO3 intermediate cores were dissolved by acid, the MnO2 shells were formed. Calcining these MnO2 shells at 500 °C, polycrystalline Mn2O3 hollow structures were obtained. The morphologies of these hollow structures were similar to their precursors. The as-prepared hollow Mn2O3 materials were used as adsorbents in water treatment, and the hollow Mn2O3 spheres, cubes, ellipsoids, and dumbbells could respectively remove about 77%, 83%, 81%, and 78% of phenol

Calibration of a Passive Sampler for Both Gaseous and Particulate Phase Polycyclic Aromatic Hydrocarbons

A novel passive air sampler was designed and tested that individually collects the gaseous and particulate phase polycyclic aromatic hydrocarbons (PAHs) in air. The sampler was calibrated against a conventional active sampler in an indoor environment. A PUF (polyurethane foam) disk and a piece of GFF (glass fiber filter) were installed in a sampling shelter for collecting gaseous and particulate phase PAHs, respectively. The passive samplers were deployed in seven indoor locations for 86 days. Six times during this period, 24-h conventional active sampling was conducted for calibration at an average interval of 17-days. Principle component analysis showed that the measured congener profile compositions were totally different between the gaseous and particulate phase PAHs, but similar between the passive and the active samples. This suggested that gaseous and particulate phase PAHs were primarily trapped by the PUF disk and GFF, respectively. Linear relationships between the passively and the actively measured and log-transformed concentrations were derived for calibration of both gaseous and particulate phase PAHs. The uptake rates of the sampler were 0.10 ± 0.014 m3/d and 0.007 ± 0.001 m3/d for gaseous and particulate phase PAHs, respectively. The rates were significantly lower than those reported in the literature using similar PUF samplers, mainly because of the special design with limited air circulation