MOESM1 of Overexpression of a type III PKS gene affording novel violapyrones with enhanced anti-influenza A virus activity

Additional file 1: Table S1. Plasmids and strains used in this study. Table S2. Primer pairs used in this study. Table S3. Homologous locus of vioAB in different Streptomyces genomes. Figure S1. Relative yields for compounds 1–14 in different strains. Figure S2. Spectral data of 1. Figure S3. Spectral data of 2. Figure S4. Spectral data of 3. Figure S5. Spectral data of 4. Figure S6. Spectral data of 5. Figure S7. Spectral data of 6. Figure S8. Spectral data of 7. Figure S9. Spectral data of 8. Figure S10. Spectral data of 9. Figure S11. Spectral data of 10. Figure S12. Spectral data of 11. Figure S13. Spectral data of 12. Figure S14. Spectral data of 13. Figure S15. Spectral data of 14. Figure S16. Multiple-sequence alignments of VioA with selected type III PKSs. Figure S17. Site-directed mutagenesis study of VioA

Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Analysis and Pharmacokinetic Assessment of Ponatinib in Sprague–Dawley Rats

Article full text The full text of this article can be found here. https://link.springer.com/article/10.1007/s40487-016-0022-2 Provide enhanced content for this article If you are an author of this publication and would like to provide additional enhanced content for your article then please contact [email protected]. The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content. Other enhanced features include, but are not limited to: • Slide decks • Videos and animations • Audio abstracts • Audio slides</p

Centrosymmetric Rb₂Sb(C₂O₄)_2.5(H₂O)₃ and Noncentrosymmetric RbSb₂(C₂O₄)F₅: Two Antimony (III) Oxalates as UV Optical Materials

Herein, we have successfully synthesized two rubidium antimony (III) oxalates, namely, Rb2Sb­(C2O4)2.5(H2O)3 and RbSb2(C2O4)­F5, utilizing a low-temperature hydrothermal method. These two compounds share a similar chemical composition, consisting of Sb3+ cations with active lone pair electrons, alkali metal Rb+ ions, and planar π-conjugated C2O42– anions. However, they exhibit different symmetries, Rb2Sb­(C2O4)2.5(H2O)3 is centrosymmetric (CS), while RbSb2(C2O4)­F5 is noncentrosymmetric (NCS), which should be caused by the presence of F– ions. Notably, the NCS compound, RbSb2(C2O4)­F5, demonstrates a moderate second-harmonic generation (SHG) response, approximately 1.3 times that of KH2PO4 (KDP), and exhibits a large birefringence of 0.09 at 546 nm. These characteristics indicate that RbSb2(C2O4)­F5 holds promising potential as a nonlinear optical material for ultraviolet (UV) applications. Detailed structural analysis and theoretical calculations confirm that the excellent optical properties arise from the synergistic effects between Sb3+ cations with SCALP and planar π-conjugated [C2O4]2– groups

Table_3_Repeated mechanical damage enhanced Aquilaria sinensis resistance to Heortia vitessoides through jasmonic acid.docx

IntroductionThe leaf-chewing pest Heortia vitessoides severely threatens the growth and development of Aquilaria sinensis. In our previous study, we found that mechanical damage (MD) to stem enhanced A. sinensis sapling resistance to H. vitessoides larvae.MethodsTo reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).ResultsHere, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.DiscussionTaken together, we provide evidence that the JA pathway plays a dominant role in the A. sinensis response to MD and HW.</p

DataSheet_1_Repeated mechanical damage enhanced Aquilaria sinensis resistance to Heortia vitessoides through jasmonic acid.docx

IntroductionThe leaf-chewing pest Heortia vitessoides severely threatens the growth and development of Aquilaria sinensis. In our previous study, we found that mechanical damage (MD) to stem enhanced A. sinensis sapling resistance to H. vitessoides larvae.MethodsTo reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).ResultsHere, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.DiscussionTaken together, we provide evidence that the JA pathway plays a dominant role in the A. sinensis response to MD and HW.</p

Table_2_Repeated mechanical damage enhanced Aquilaria sinensis resistance to Heortia vitessoides through jasmonic acid.xls

IntroductionThe leaf-chewing pest Heortia vitessoides severely threatens the growth and development of Aquilaria sinensis. In our previous study, we found that mechanical damage (MD) to stem enhanced A. sinensis sapling resistance to H. vitessoides larvae.MethodsTo reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).ResultsHere, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.DiscussionTaken together, we provide evidence that the JA pathway plays a dominant role in the A. sinensis response to MD and HW.</p

Table_1_Repeated mechanical damage enhanced Aquilaria sinensis resistance to Heortia vitessoides through jasmonic acid.xls

IntroductionThe leaf-chewing pest Heortia vitessoides severely threatens the growth and development of Aquilaria sinensis. In our previous study, we found that mechanical damage (MD) to stem enhanced A. sinensis sapling resistance to H. vitessoides larvae.MethodsTo reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).ResultsHere, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.DiscussionTaken together, we provide evidence that the JA pathway plays a dominant role in the A. sinensis response to MD and HW.</p