Additional file 2: of Spliceosomal introns in Trichomonas vaginalis revisited

Table S2. An update on the gene ID of the 42 introns previously reported, with references as indicated [15, 16]. (PDF 77 kb

Additional file 3: of Spliceosomal introns in Trichomonas vaginalis revisited

Figure S1. Experimental validation of the 62 T. vaginalis putative introns by RT-PCR. These introns were categorised as (a) functional, (b) non-functional or (c) undetermined. (PDF 256 kb

Additional file 5: of Spliceosomal introns in Trichomonas vaginalis revisited

Table S4. T. vaginalis genome segments that match the consensus sequence of type B intron (GTWYWDN{7}TCTAACH{1,2}AACAG). (PDF 85 kb

Additional file 4: of Spliceosomal introns in Trichomonas vaginalis revisited

Table S3. T. vaginalis genome segments that match the consensus sequence of type A intron (GYAYGYN{41,178}RCTAACACAYAG). (PDF 84 kb

Additional file 1: of Spliceosomal introns in Trichomonas vaginalis revisited

Table S1. Primers used in this study to validate the putative introns by RT-PCR. Nucleotides that target 5' and 3' UTRs were indicated with lower-case letters. Underlined bases are located outside the transcribed region of these genes. (PDF 36 kb

Two new 2-arylbenzofurnan derivatives from the leaves of <i>Morus alba</i>

<p>Two new 2-arylbenzofuran derivatives, moracinfurol A and B (<b>1</b>–<b>2</b>), and ten known compounds (<b>3</b>–<b>12</b>) were isolated from the leaves of <i>Morus alba.</i> Their structures were determined on the basis of spectroscopic analysis including 1D, 2D NMR and HR-ESI-MS. All of the 2-arylbenzofuran derivatives were evaluated for cytotoxicity against A549 cells. Some cytotoxic 2-arylbenzofuran derivatives might induce autophagy characterized by the accumulation of LC-3 Ⅱ.</p

Additional file 6: of Spliceosomal introns in Trichomonas vaginalis revisited

Figure S2. Evidence of gene transcription and mRNA splicing for the 13 putative introns that could not be experimentally determined in this study. (PDF 1422 kb

Data_Sheet_1_Comparsion of Activities of Fatty Acyl Desaturases and Elongases Among Six Teleosts With Different Feeding and Ecological Habits.docx

Fatty acyl desaturases 2 (Fads2) and elongases of very-long-chain fatty acid 5 (Elovl5) are two key enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs), and their activities determine the LC-PUFA biosynthetic ability of teleost. In order to investigate the relation of enzymic activities with fish’s feeding habits and ecological habits, the activities of Fads2 and Elovl5 were compared among six teleosts, namely, freshwater carnivorous mandarin fish (Siniperca chuatsi), freshwater herbivorous grass carp (Ctenopharyngodon idellus), marine carnivorous orange-spotted grouper (Epinephelus coioides), marine herbivorous rabbitfish (Siganus canaliculatus), anadromous Atlantic salmon (Salmo salar), and catadromous Japanese eel (Anguilla japonica). Among them, the enzymatic features of Fads2 and Elovl5 from the last five fish species have been characterized, whereas those of S. chuatsi were unknown. And thus, the coding sequences (CDSs) of S. chuatsi fads2 and elovl5 (elovl5a and elovl5b) were isolated, and their functions were further characterized by heterologous expression in yeast. The results showed that S. chuatsi Fads2 has a monofunctional Δ6 desaturase and that Elovl5a has a higher activity toward C18–C20 PUFAs than has Elovl5b, which showed a noteworthy activity toward C22 PUFAs. The comparison of enzymatic activities among the six teleosts showed that the Δ6 Fad and Elovl5 activities varied markedly among fish species; in particular, the activity of Δ6 Fad in C. idellus, S. canaliculatus, and A. japonica was significantly higher than that in S. chuatsi, S. salar, and E. coioides. For C18 PUFA substrates, A. japonica Elovl5 has a higher elongation than has the other tested fish, and it exhibits a higher activity toward the C20 PUFAs. The results suggest that the Δ6 Fad activity is influenced by both feeding habits and ecological habits, whereas the Elovl5 activity was more affected by the feeding habits. These data enrich our knowledge on LC-PUFA biosynthesis diversity of fatty acid desaturation and elongations in teleosts and provide guidance for the choice of dietary PUFA precursors for farmed fish.</p

Additional file 3 of Nonviral mcDNA-mediated bispecific CAR T cells kill tumor cells in an experimental mouse model of hepatocellular carcinoma

Additional file 3

Additional file 2 of Nonviral mcDNA-mediated bispecific CAR T cells kill tumor cells in an experimental mouse model of hepatocellular carcinoma

Additional file 2