Additional file 7 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 7. Melting curves in real time PCR

Additional file 5 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 5. Original images for Fig. 4a, Fig. 5a and Fig. 6b

Additional file 4 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 4. Photobleaching phenotype of peppers infiltrated with TRV:CaPDS for 15 d

Additional file 1 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 1. Schematic diagram of CaCRK5 protein domain architecture. It contains the signal peptide (SP), two domains 26 of unknown function (DUF26), transmembrane domain (TM) and kinase domain

Additional file 3 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 3. Chromosomal location and phylogenetic analysis of CRK gene family. a Physical genome distribution ofCRK gene family in pepper. b Phylogenetic tree was generated based on the amino acid sequences of CRK genes in pepper using the neighbor-joining method with 1000 bootstrap replicates in MEGA version 7.0

Nuclear localization of <i>Os</i>PIANK1 protein in <i>N. benthamiana</i> leaves.

<p><i>Os</i>PIANK1–GFP exclusively localized in the nucleus of cells in <i>N. benthamiana</i> leaves. Only the green fluorescent (GFP) localized throughout the whole cells. Cells were detected for GFP fluorescence by fluorescence microscopy 48 h after agroinfiltration.</p

<i>OsPIANK1</i> promoter activation in response to inoculation with <i>M.oryzae</i> or exogenous application of MeJA, SA or ABA.

<p>The leaves were harvested at 6 days after inoculation with <i>M.oryzae</i> and 12 h after treatment with the phytohormones. The numbers over the bars indicate the increase in induction of GUS activity compared to the mock. Data represent the means ± SE from the leaf extracts collected from three experimental plant units. (**, *indicate significant differences between the untreated (mock) and treated plants at <i>P</i><0.05 and <i>P</i><0.01, respectively, as determined by the SNK test.).</p

Nucleotide sequence of 5′ flanking promoter regions and stress-related sequence motifs putatively acting as <i>cis</i>-elements of the <i>OsPIANK1</i> gene.

<p>HSE: <i>cis</i>-acting element involved in heat stress responsiveness. CGTCA-box: <i>cis</i>-acting regulatory element involved in the MeJA-responsiveness. JERE: <i>cis</i>-acting regulatory element involved in the MeJA-responsiveness. ERE: ethylene-responsive element. DRE: <i>cis</i>-acting element involved in dehydration, low-temp, salt stresses. MBS: MYB binding site involved in drought-inducibility. GCC-box: ethylene-responsive element. ABRE: <i>cis</i>-acting element involved in the abscisic acid responsiveness. TCA-element: <i>cis</i>-acting element involved in salicylic acid responsiveness.</p

Additional file 2 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 2. Essential information of CRKs gene family in pepper

Additional file 6 of A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Additional file 6. The information of realt time PCR primers