Data_Sheet_1_Epidemiological characteristics and prediction model construction of hemorrhagic fever with renal syndrome in Quzhou City, China, 2005–2022.docx

BackgroundHemorrhagic fever with renal syndrome (HFRS) is one of the 10 major infectious diseases that jeopardize human health and is distributed in more than 30 countries around the world. China is the country with the highest number of reported HFRS cases worldwide, accounting for 90% of global cases. The incidence level of HFRS in Quzhou is at the forefront of Zhejiang Province, and there is no specific treatment for it yet. Therefore, it is crucial to grasp the epidemiological characteristics of HFRS in Quzhou and establish a prediction model for HFRS to lay the foundation for early warning of HFRS.MethodsDescriptive epidemiological methods were used to analyze the epidemic characteristics of HFRS, the incidence map was drawn by ArcGIS software, the Seasonal AutoRegressive Integrated Moving Average (SARIMA) and Prophet model were established by R software. Then, root mean square error (RMSE) and mean absolute error (MAE) were used to evaluate the fitting and prediction performances of the model.ResultsA total of 843 HFRS cases were reported in Quzhou City from 2005 to 2022, with the highest annual incidence rate in 2007 (3.93/100,000) and the lowest in 2022 (1.05/100,000) (P trendConclusionFrom 2005 to 2022, the incidence of HFRS in Quzhou City showed an overall downward trend, but the epidemic in high-incidence areas was still serious. In the future, the dynamics of HFRS outbreaks and host animal surveillance should be continuously strengthened in combination with the Prophet model. During the peak season, HFRS vaccination and health education are promoted with farmers as the key groups.</p

Synthesis and Self-Assembly of Perylenetetracarboxylic Diimide Derivatives with Helical Oligo(l‑lactic acid)_<i>n</i> Segments

Three perylenetetracarboxylic diimide (PDI) derivatives consisting of a short oligo­(l-lactic acid)_<i>n</i> (O-LLA) segment at one imide nitrogen were synthesized. The polymers were characterized by ¹H NMR and gel permeation chromatography (GPC). Their properties were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD) experiments, scanning electron microscopy (SEM), electronic absorption, and circular dichroism (CD) spectroscopy. The self-assembly behavior of these PDIs in molten state as well as in solvent was examined. It was found that the structure and the morphology of the self-assembly of these polymers depend on the relative length of the O-LLA segment. The PDIs with longer O-LLA chains present liquid crystal properties with an obvious phase transition from disordered phase to an ordered (α) phase, which cannot be found for the PDIs with short O-LLA segments. The long O-LLA segments also caused a left-handed helicity for the aggregates of the PDIs from solution. This research demonstrated that one can control the order, aggregation mode, and morphology of the molecular aggregates by changing the length of the O-LLA chains. This information can be useful in the design of new organic materials that exhibit molecular aggregation

Specific Imaging of Tyrosinase in Vivo with 3‑Hydroxybenzyl Caged D‑Luciferins

Tyrosinase (TYR), a key enzyme in biosynthesis of melanin, usually functions as a biomarker of severe skin diseases such as vitiligo and melanoma cancer. Accurate detection of TYR activity in vivo is urgent but still challenging. Inspired by the advantages of bioluminescence in vivo strategy in imaging and the specific hydroxylation of 3-hydroxybenzyloxy group by TYR, a bioluminogenic probe, TYR–LH₂, was designed and synthesized through caging D-luciferin with 3-hydroxybenzyl. The probe exhibits high selectivity and sensitivity toward TYR with a detection limit of 0.11 U/mL in a small detection volume of 100 μL. Bioluminescence imaging results show that TYR–LH₂ is fully competent for monitoring the dynamic changes of TYR in living cells and model animals and possesses the capability of discriminating melanocytes from other cell lines, thus offering a promising approach for investigation and diagnosis of melanoma cancer and other TYR-related diseases in vivo

Sensing in 15 s for Aqueous Fluoride Anion by Water-Insoluble Fluorescent Probe Incorporating Hydrogel

Anion recognition and sensing via artificial receptors have attracted a great deal of attention since they play a fundamental and important role in chemical, biological, medical, and environmental processes. Fluoride, as one of the smallest anions, is of particular interest because of its role in dental care and the analysis of drinking water. Herein, we invented a new method for F^– detection by adopting the hydrogel as the supporter of reaction between a water insoluble fluorescent probe and F^– in the water environment. This method is highly rapid, selective, and sensitive, which can determine F^– levels in 15 s at the drinking water standard. A novel compound <i>N</i>-(3-(benzo­[d]­thiazol-2-yl)-4-(tert-butyldiphenylsilyloxy)­phenyl) acetamide (BTBPA) was synthesized as the fluorescent probe because of the significant fluorescent color change from blue to green after the reaction with F^–. This method does not require the probe substances to be water-soluble, which greatly expands the range of the specific fluorescent molecules used in ion detection. Additionally, just a few microliter samples were required in the analysis procedures with this method

A Single-Component Molecular Glass Resist Based on Tetraphenylsilane Derivatives for Electron Beam Lithography

A novel molecular glass (TPSiS) with photoacid generator (sulfonium salt group) binding to tetraphenylsilane derivatives was synthesized and characterized. The physical properties such as solubility, film-forming ability, and thermal stability of TPSiS were examined to assess the suitability for application as a candidate for photoresist materials. The sulfonium salt unit underwent photolysis to effectively generate photoacid on UV irradiation, which catalyzed the deprotection of the t-butyloxycarbonyl groups. It demonstrates that the TPSiS can be used as a ‘single-component’ molecular resist without any additives. The lithographic performance of the TPSiS resist was evaluated by electron beam lithography. The TPSiS resist can resolve 25 nm dense line/space patterns and 16 nm L/4S semidense line/space patterns at a dose of 45 and 85 μC/cm2 for negative-tone development (NTD). The etching selectivity of the TPSiS resist to Si substrate is 8.6 under SF6/O2 plasma, indicating a potential application. Contrast analysis suggests that the significant solubility switch within a narrow exposure dose range (18–47 μC/cm2) by NTD is favorable for high-resolution patterns. This study supplies useful guidelines for the optimization and development of single-component molecular glass resists with high lithographic performance

Molecular Engineering of Aqueous Soluble Triarylboron-Compound-Based Two-Photon Fluorescent Probe for Mitochondria H₂S with Analyte-Induced Finite Aggregation and Excellent Membrane Permeability

Hydrogen sulfide (H₂S) is a multifunctional signaling molecule that participates in many important biological processes. Herein, by functionalizing triarylboron with cyclen and diphenylamine, we synthesized TAB-1, TAB-2, and TAB-3 for H₂S recongnization by rational design of molecular structures. Among them, aqueous soluble TAB-2 possesses excellent properties, including large two-photon action cross section, membrane permeability and can effectively complex with Cu²⁺. The complex of TAB-2-Cu²⁺ can selectively detect H₂S with an instant response and mitochondria targeted. Moreover, the H₂S-induced finite aggregation of indicators enhances their photostability and causes variation of the fluorescence lifetime. TAB-2-Cu²⁺ has also been successfully applied for the mitochondria H₂S imaging in NIH/3T3 fibroblast cells by TPM and FLIM

Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly <i>N</i>‑Isopropylacrylamide and Energy Transfer

A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30–55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo

Molecular Dual-Rotators with Large Consecutive Emission Chromism for Visualized and High-Pressure Sensing

Low-cost, stable, highly sensitive, and easy-to-equip fluorescent high-pressure sensors are always attractive in both industrial and scientific communities. Organic emitting materials with pressure-dependent bathochromisms usually exhibit prominent mechanoluminescence, due to disturbance of intermolecular packing. This hinders their applications in stable and robust pressure sensing. In this work, we have developed a mechanically stable organic molecular pressure sensor, caused by intramolecular consecutive rotations by pressure, which exhibit large and eye-detectable emission bathochromism from yellow-green to red fluorescence and can be used for 0–15 GPa pressure sensing. The emission bathochromism shows good linear relationship with pressure, exhibiting a high linear coefficient of 9.1 nm/GPa. Moreover, this molecular sensor exhibits high thermal and mechanical stabilities, indicating good potentials for robust and outdoor applications