Generation and Application of a Novel InsP₃R₁ Mono-Antibody from Mouse

<div><p>Inositol 1, 4, 5-Trisphosphate Receptor (InsP₃R) is an intracellular Ca²⁺ release channel, which widely participates in cellular processes. Three isoforms of InsP₃R were identified as InsP₃R₁, InsP₃R_2, and InsP₃R₃. They share 60–0% protein sequence homology and form a channel in a manner of homotetramer or heterotetramer. Several InsP₃R isoform-specific rabbit antibodies have been generated to distinguish their localization and functions. However, there is no report of such a valid antibody raised from other species. In his article, we prepare a mouse monoclonal antibody against a synthetic peptide with rat InsP₃R₁-specific carboxyl terminus sequence. This monoclonal antibody of InsP₃R₁ (R1-mAb) was purified and characterized as IgG2b, which can recognize InsP₃R₁ by Western-blot (WB) analysis and immunoprecipitate (IP) InsP₃R₁ from moue brain lysate tested. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody could give the consistent results in SH-SY5Y cells, human sperm, and mouse brain paraffin section. In summary, we generate a mouse InsP₃R₁-specific IgG 2b antibody identifying InsP₃R₁ in WB, IF, IHC, and IP analysis, which provides a possible choice for detection of InsP₃R₁, especially in application of co-localization analysis with other InsP₃R isoforms or other proteins. </p></div

Mechanistic Insights Into the Rhodium-Catalyzed C–H Alkenylation/Directing Group Migration and [3+2] Annulation: A DFT Study

The mechanism of the rhodium-catalyzed C–H alkenylation/directing group migration and [3+2] annulation of N-aminocarbonylindoles with 1,3-diynes has been investigated with DFT calculations. On the basis of mechanistic studies, we mainly focus on the regioselectivity of 1,3-diyne inserting into the Rh–C bond and the N-aminocarbonyl directing group migration involved in the reactions. Our theoretical study uncovers that the directing group migration undergoes a stepwise β-N elimination and isocyanate reinsertion process. As studied in this work, this finding is also applicable to other relevant reactions. Additionally, the role of Na+ versus Cs+ involved in the [3+2] cyclization reaction is also probed

Data_Sheet_1_Quantum Dots-Based Immunochromatographic Strip for Rapid and Sensitive Detection of Acetamiprid in Agricultural Products.docx

In this study, a rapid and sensitive immunochromatographic strip (ICS) assay, based on quantum dots (QDs), was developed for the qualitative and quantitative detection of acetamiprid in agricultural samples. Acetamiprid-ovalbumin conjugates (ACE-OVA) and goat anti-mouse IgG were sprayed onto a nitrocellulose membrane as a test and control line. Two kinds of anti-acetamiprid monoclonal antibodies (mAb) obtained in our lab were characterized by the ELISA and surface plasmon resonance assay. The competitive immunoassay was established using a QDs-mAb conjugate probe. The visual detection limit of acetamiprid for a qualitative threshold was set as 1 ng/mL to the naked eye. In the quantitative test, the fluorescence intensity was measured by a portable strip reader and a standard curve was obtained with a linear range from 0.098 to 25 ng/mL, and the half maximal inhibitory concentration of 1.12 ng/mL. The developed method showed no evident cross-reactivities with other neonicotinoid insecticides except for thiacloprid (36.68%). The accuracy and precision of the developed QDs-ICS were further evaluated. Results showed that the average recoveries ranged from 78.38 to 126.97% in agricultural samples. Moreover, to test blind tea samples, the QDs-ICS showed comparable reliability and a high correlation with ultra-performance liquid chromatography-tandem mass spectrometry. The whole sample detection could be accomplished within 1 h. In brief, our data clearly manifested that QDs-ICS was quite qualified for the rapid and sensitive screening of acetamiprid residues in an agricultural product analysis and paves the way to point-of-care testing for other analytes.</p

Solution-Processed Sb₂S₃ Planar Thin Film Solar Cells with a Conversion Efficiency of 6.9% at an Open Circuit Voltage of 0.7 V Achieved via Surface Passivation by a SbCl₃ Interface Layer

Interfaces in Sb2S3 thin-film solar cells strongly affect their open-circuit voltage (VOC) and power conversion efficiency (PCE). Finding an effective method of reducing the defects is a promising approach for increasing the VOC and PCE. Herein, the use of an inorganic salt SbCl3 is reported for post-treatment on Sb2S3 films for surface passivation. It is found that a thin SbCl3 layer could form on the Sb2S3 surface and produce higher efficiency cells by reducing the defects and suppressing nonradiative recombination. Through density functional theory calculations, it is found that the passivation of the Sb2S3 surface by SbCl3 occurs via the interactions of Sb and Cl in SbCl3 molecules with S and Sb in Sb2S3, respectively. As a result, incorporating the SbCl3 layer highly improves the VOC from 0.58 to 0.72 V; an average PCE of 6.9 ± 0.1% and a highest PCE of 7.1% are obtained with an area of 0.1 cm2. The achieved PCE is the highest value in the Sb2S3 planar solar cells. In addition, the incorporated SbCl3 layer also leads to good stability of Sb2S3 devices, by which 90% of the initial performance is maintained for 1080 h of storage under ambient humidity (85 ± 5% relative humidity) at room temperature

Mechanistic Insights Into the Rhodium-Catalyzed C–H Alkenylation/Directing Group Migration and [3+2] Annulation: A DFT Study

The mechanism of the rhodium-catalyzed C–H alkenylation/directing group migration and [3+2] annulation of N-aminocarbonylindoles with 1,3-diynes has been investigated with DFT calculations. On the basis of mechanistic studies, we mainly focus on the regioselectivity of 1,3-diyne inserting into the Rh–C bond and the N-aminocarbonyl directing group migration involved in the reactions. Our theoretical study uncovers that the directing group migration undergoes a stepwise β-N elimination and isocyanate reinsertion process. As studied in this work, this finding is also applicable to other relevant reactions. Additionally, the role of Na+ versus Cs+ involved in the [3+2] cyclization reaction is also probed