Rh(III)-Catalyzed Direct <i>ortho</i>-Chalcogenation of Phenols and Anilines

A highly efficient Rh­(III)-catalyzed direct C–H chalcogenation of phenols and anilines has been achieved with the assistance of the 2-pyridyl group. The reaction features a broad substrate scope, good functional group tolerance, complete monothiolation selectivity, and easily removable directing group

Cu-Catalyzed Direct Amidation of Aromatic C–H Bonds: An Access to Arylamines

A Cu-catalyzed aromatic C–H amidation with phthalimide under oxygen as a terminal oxidant without using additional additives has been achieved. This reaction has the broad substrate scope and shows moderate to good yields in most cases. This method is complementary to the previously reported metal-catalyzed C–H amination systems

DataSheet_1_CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR.pdf

Distant metastasis is the main cause of death in nasopharyngeal carcinoma (NPC) patients. There is an urgent need to reveal the underlying mechanism of NPC metastasis and identify novel therapeutic targets. The ferroptosis resistance and survival ability of extracellular matrix (ECM)-detached tumor cells are important factors in determining the success of distant metastasis. In this study, we found that CAPRIN2 contributes to the ferroptosis resistance and survival of ECM-detached NPC cells. Moreover, CAPRIN2 serves as a positive regulator of NPC cell migration and invasion. HMGCR, the key metabolic enzyme of the mevalonate pathway, was identified as the key downstream molecule of CAPRIN2, which mediates its regulation of ferroptosis, survival, migration and invasion of NPC cells. Lung colonization experiments showed that downregulation of the CAPRIN2/HMGCR axis resulted in reduced lung metastasis of NPC cells. Erastin treatment inhibited the ability of NPC cells to colonize the lungs, which was further enhanced by CAPRIN2/HMGCR axis downregulation. Regulated by upstream LINC00941, CAPRIN2 is abnormally activated in NPC, and its high expression is associated with a poor prognosis. In conclusion, CAPRIN2 is a molecular marker of a poor prognosis in NPC, and the LINC00941/CAPRIN2/HMGCR axis provides a new target for the treatment of NPC metastasis and ferroptosis resistance.</p

Two in One: Luminescence Imaging and 730 nm Continuous Wave Laser Driven Photodynamic Therapy of Iridium Complexes

A phosphorescent cationic iridium complex (<b>1</b>) with a donor−π-donor type unit as a N^∧N ligand and 2-phenylpyridine as a C^∧N ligand was synthesized and characterized. <b>1</b> exhibits an intense absorption peak centered at ∼450 nm with a large molar extinction coefficient (ε) of ∼2.4 × 10⁴ M^–1 cm^–1 and displays highly efficient orange-red phosphorescence located at 620 nm with a quantum efficiency of ∼3% in PBS buffer solution at room temperature, which is beneficial for a luminescence probe in living cells excited by visible light. Importantly, the generation of singlet oxygen (¹O₂) by <b>1</b> was observed by 730 nm continuous wave (CW) laser irradiation owing to its reverse saturable absorption property, which was confirmed by the ¹O₂ scavenger of 1,3-diphenylbenzo­[<i>c</i>]­furan (DPBF) and ESR spectra. The photodynamic therapy effect of <b>1</b> in vivo by a 730 nm CW laser was further assessed by histological examination and immunohistochemistry

Synthesis, crystal structure, and application as a Ag⁺ chemodosimeter of phosphorescent iridium complexes

<div><p>The syntheses and crystal structures of two iridium complexes, (dfppy)₂IrPyCl (<b>1</b>) and [(dfppy)₂Ir(Py)₂]PF₆ (<b>2</b>), are reported. <b>1</b> can selectively detect Ag⁺ with UV–vis absorption and emission spectra. In the presence of Ag⁺, the obvious decrease of the luminescence intensity at 476 nm was observed, which could be monitored by the naked eyes. The phosphorescence quantum yield decreases from 0.024 to 0.012. No obvious changes of the luminescence intensity were observed upon addition of a large excess of other transition metal ions. Due to the strong interaction between chloride and Ag⁺, the special chemical reaction induced by Ag⁺ is responsible for the significant change of absorption and luminescence spectra.</p></div

Phosphorescent Coordination Polymer Nanoparticles as a Three-in-One Platform for Optical Imaging, T₁‑Weighted Magnetic Resonance Imaging, and Photodynamic Therapy

Water-soluble coordination polymer nanoparticles (Ir–Gd CPPs) were conveniently synthesized in high yields in the presence of polyvinylpyrrolidone (PVP), adopting magnetic Gd­(III) ions, and phosphorescent iridium complexes with carboxyl groups as building blocks. The Ir–Gd CPPs showed good stability in simulated biological media and low cytotoxicity toward a model line of HeLa cancer cells. The Ir–Gd CPPs exhibited absorption in the visible region, red phosphorescence centered at 560 nm, and higher longitudinal relaxivity (<i>r</i>₁) of ∼29.5 mM^–1 s^–1 in a 3 T MRI system. Furthermore, the effective uptake of Ir–Gd CPPs by HeLa cells was confirmed by confocal laser scanning microscopy and flow cytometry, suggesting they may be useful as an optical probe for living cells. The generation of ¹O₂ upon irradiation with a visible light prompted an investigation into the possibility of using Ir–Gd CPPs in photodynamic therapy. After incubation with 200 μg mL^–1 of Ir–Gd CPPs for 6 h, the viability of HeLa cells was only ∼16.6% after irradiation with a visible light (λ > 400 nm, 300 mW cm^–2)

AraPathogen2.0: An Improved Prediction of Plant–Pathogen Protein–Protein Interactions Empowered by the Natural Language Processing Technique

Plant–pathogen protein–protein interactions (PPIs) play crucial roles in the arm race between plants and pathogens. Therefore, the identification of these interspecies PPIs is very important for the mechanistic understanding of pathogen infection and plant immunity. Computational prediction methods can complement experimental efforts, but their predictive performance still needs to be improved. Motivated by the rapid development of natural language processing and its successful applications in the field of protein bioinformatics, here we present an improved XGBoost-based plant–pathogen PPI predictor (i.e., AraPathogen2.0), in which sequence encodings from the pretrained protein language model ESM2 and Arabidopsis PPI network-related node representations from the graph embedding technique struc2vec are used as input. Stringent benchmark experiments showed that AraPathogen2.0 could achieve a better performance than its precedent version, especially for processing the test data set with novel proteins unseen in the training data

Effect of prognostic factors on survival in multivariable analyses.

<p>* Age, gender, cigarette smoking, alcohol use, T stage, N stage, rT stage, rN stage, AC cycles and treatment method in multivariate Cox mode.</p><p>Effect of prognostic factors on survival in multivariable analyses.</p

Kaplan-Meier survival curves for 155 patients with residual tumors after undergoing CCRT without or with AC.

<p>Overall survival (A), failure-free survival (B), local relapse-free survival (C), and distant metastasis-free survival (D). P values were calculated with the unadjusted log-rank test. CCRT = concurrent chemoradiotherapy; AC = adjuvant chemotherapy.</p