A cross-cultural study of domestic luminous environment in the United Kingdom and Japan

Abstract not available

Multiple sequence alignment and phylogenetic analysis of <i>BnPRP1</i>.

<p>(A) Unrooted Phylogenetic tree of BnPRP1 and its homologous proteins. Dendrogram obtained using neighbor-joining analysis based on the proportion (p-distance) of aligned amino acid sites of the full-length peptide sequences. BnPRP1 is marked with a solid triangle. Numbers at the base of each clade correspond to the bootstrap means of 1000 replications. Organism’s common or taxonomic names are shown in parenthesis. The Genbank accession numbers/Database accession numbers for the proteins/peptides are as follows: <i>Arabidopsis thaliana</i> (NP_197850.2); <i>Capsella rubella</i> (XP_006289819); <i>Camelina sativa</i> (XP_010421292.1); <i>Arabidopsis lyrata</i> (XP_00287212); <i>Eutrema salsugineum</i> (XP_0063947); <i>Brassica napus</i> (<i>Brassica napus</i> Database accession No. GSBRNA2T00120298001); <i>Brassica oleracea</i> (bolbase accession No: Bol016440); <i>Brassica rapa</i> (brad accession No: XP_009130006.1); <i>Brassica napus</i> (<i>Brassica napus</i> Database accession No:GSBRNA2T00147553001); <i>Brassica rapa</i> (brad accession No: XP_009150986.1); <i>Brassica napus</i> (GSBRNA2T00133542001); <i>Brassica oleracea</i> (bolbase accession No: Bol022411); <i>Brassica napus</i> (<i>Brassica napus</i> Database accession No: GSBRNA2T00120360001); SP-B (APD accession No: AP00889); BacFL31(APD accession No: AP02346); bumblebee_Abaecins (APD accession No: AP01215); honeybee_Abaecins (APD accession No: AP00002). (B) Multiple alignments of BnPRP1 and its homologs. Multiple alignments of BnPRP1 and its homologs identical amino acid residues were black-shaded, PPT motif of BnPRP1 is shown in box.</p

Minimal concentrations of <i>BnPRP1</i> required for complete growth inhibition.

<p>^aResults are the mean values obtained from three independent measurements.</p><p>Minimal concentrations of <i>BnPRP1</i> required for complete growth inhibition.</p

Structural data of <i>BnPRP1</i> obtained from CD.

<p>^aPBS, pH 7.4</p><p>TFE⁺, trifluoroethanol; MeOH, methanol; EtOH, ethanol</p><p>Structural data of <i>BnPRP1</i> obtained from CD.</p

SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1 expressed in <i>E</i>. <i>coli</i> BL 21 (DE3) and Tricine-SDS-PAGE analysis of BnPRP1 renaturation.

<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; Lanes 2, precipitation of His-EDDIE-BnPRP1; Lane 1, <i>E</i>. <i>coli</i> BL 21 (DE3) cells transformed with pET30a plasmid as a control. Lane 3, purified His-EDDIE-BnPRP1. (B) Tricine-SDS-PAGE analysis of BnPRP1 renaturation. Lane M, protein molecular weight marker; Lanes 1, 2, refolded BnPRP1 after 8 h.</p

Phylogenetic tree of all bZIP proteins from Arabidopsis thaliana and six legume genomes

The PDF file was the phylogenetic tree of all bZIP proteins from Arabidopsis thaliana and six legume genomes, which corresponds to the Figure 1 and Additional file 3 in publicatio

Agarose gel electrophoresis detection of the overlap PCR product from the <i>BnPRP1</i> gene and the recombinant plasmids pET30a/His-EDDIE-GFP and pET30a-EDDIE-BnPRP1.

<p>(A) Lane M, 100 bp DNA marker; Lanes 1–2, Overlap PCR products from the <i>BnPRP1</i> gene. (B) Lane M, 1 kb DNA marker; Lane 1, recombinant vector pET30a/His-EDDIE-BnPRP1; Lane 2, vector pET30a/His-EDDIE-GFP. <b>C:</b> Lane M, DL2000 DNA marker; Lane 1, positive control; Lane 2, negative control; Lanes 3 and 4, PCR detection of the <i>BnPRP1</i> gene.</p

CD spectra of <i>BnPRP1</i> in different solvents (A) and in the presence of 0–75% (v/v) TFE (B).

<p>CD spectra of <i>BnPRP1</i> in different solvents (A) and in the presence of 0–75% (v/v) TFE (B).</p

Genomic distribution of microsatellites as well as genes and TEs in the assembled pseudochromosomes of several sequenced angiosperm species, i.e., <i>B. rapa</i> (A), <i>B. oleracea</i> (B), <i>A. thaliana</i> (C), <i>G. max</i> (D), <i>M. truncatula</i> (E), <i>V. vinifera</i> (F), <i>S. bicolor</i> (G), <i>Z. mays</i> (H), <i>O. sativa</i> (I) and <i>B. distachyon</i> (J).

<p>The horizontal axis shows the assembled pseudochromosomes, which were divided into 1-Mb intervals. The left and right vertical-axis shows the frequency of microsatellites/genes and TEs, respectively. On the figure: the lines of different colors represent the distribution of microsatellites (black), genes (blue) and TEs (red), respectively; the lines of different types represent actual (solid) and hypothetical/even (dashed) distribution, respectively.</p

Raw protein sequences of all identified bZIPs in six legume genomes

This file includes the bZIPs protein sequences from Arabidopsis thaliana and six legume genomes (Glycine max,Medicago truncatula,Cajanus cajan,Cicer arietinum,Phaseolus vulgaris and Lotus japonicus). All these proteins were used to construct phylogenetic tree which correspondence to Figure 1 and Additional file 3 of publication