7 research outputs found
Additional file 6: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Table S2. miRNAs associated with UCA1. (DOCX 13 kb
Additional file 8: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Figure S5. Effect of UCA1 on the invasive abilities of MCF7 cells. Histograms show the effect of UCA1 on the invasive abilities of MCF7 cells. Values represent the means ± SD from three independent experiments; **P < 0.01, *P < 0.05 as determined by one-way ANOVA followed by Tukey’s multiple comparison tests. (TIFF 992 kb
Additional file 1: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Table S1. Primers used for qPCR, RT-PCR and siRNA interference. (DOC 53 kb
Additional file 5: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Figure S3. UCA1 is associated with IMP1 and CNOT1 and with miR-185-5p. (A) Vectors expressing UCA1 or UCA1-MS2 were transiently transfected into MDA231/IMP1-GFP cells. Pulldown assays were performed to analyze the association of IMP1 and CNOT1 with UCA1-MS2. Representative images indicate that both IMP1 and CNOT1 co-precipitated with UCA1. Control: cells transfected with MS2-untagged UCA1. (B) Putative binding site of UCA1 for miR-185-5p. (C) Interaction of miR-185-5p with UCA1-MS2 was examined in the pulldown material. Relative levels of miR-185-5p in the precipitates were statistically analyzed as means ± SD from three independent experiments: **P < 0.01 as determined by Student’s t test. (TIFF 1227 kb
Additional file 7: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Figure S4. IMP1 knockdown increases the expression of miR-122-5p target mRNAs. (A) Cellular levels of miR-122-5p are not affected by IMP1-GFP expression. (B) After MS2 pulldown experiments, levels of miR-122-5p in the supernatants were analyzed by qPT-PCR. Levels of miR-122-5p were normalized to GAPDH mRNA from three independent experiments: **P < 0.01 as determined by Student’s t test. (C) RT-qPCR was applied to measure the levels of PKM2 and IGF-1R mRNAs in IMP1 knockdown T47D cells. Levels of the mRNAs were normalized to GAPDH mRNA from three independent experiments: *P < 0.05 as determined by Student’s t test. (TIFF 884 kb
Additional file 9: of IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p
Figure S6. A proposed model of IMP1 to regulate the sponge effect of UCA1 for miR-122-5p. (A) UCA1 sponges miR-122-5p, reducing miR-122-5p interaction with target mRNA. (B) Increasing IMP1 expression allows IMP1 to bind to UCA1 and to release miR-122-5p from UCA1. This increases the availability of miR-122-5p to interact with target mRNA. (C) Binding to target mRNA allows miR-122-5p to assert its posttranscriptional function. (D) IMP1 binds to UCA1 and recruits it to the CCR4-NOT1 complex, initiating UCA1 decay process. (TIFF 1116 kb