24 research outputs found

    Isobavachalcone Attenuates MPTP-Induced Parkinson's Disease in Mice by Inhibition of Microglial Activation through NF-κB Pathway

    No full text
    <div><p>Parkinson's disease (PD) is a complex multi-system and age-related neurodegenerative disorder. The intervention targeting neuroinflammation in PD patients is one effective strategy to slow down or inhibit disease progression. Microglia-mediated inflammatory response plays an important role in Parkinson's, Alzheimer's and other cerebral diseases. Isobavachalcone is a main component of Chinese herb medicine <i>Psoralea corylifolia</i>, which function includes immunoregulation, anti-oxidation and the regulation of β-amyloid (Aβ42) deposited in hippocampus in Alzheimer's patients. Whether it has the therapeutic effect on Parkinson's disease, however, is unclear. In this study, we found that isobavachalcone could effectively remit Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP), prolong the residence time of mice on Rota-rod and alleviate the neuronal necrosis. It also inhibited the over-activation of microglia, and decreased the expression of IL-6 and IL-1β in the brain of PD mice. In vitro, isobavachalcone could inhibit nuclear factor-kappaB (NF-κB) pathway through inhibiting the LPS-induced transfer of NF-κB subunit from cytoplasm to nucleus in BV-2 cells. Isobavachalcone decreased the LPS-induced oxidative stress and the expression of inflammatory cytokines, and provided a neuroprotective effect by antagonizing microglia-mediated inflammation. Our results indicated that isobavachalcone may be a candidated drug against Parkinson's disease with great clinical potential.</p></div

    Effects of isobavachalcone treatment on BV-2 cells and Neuro-2a cells.

    No full text
    <p>A: Isobavachalcone (Iso) caused no significant cytotoxicity to BV-2 cells. B: The effect of isobavachalcone treated BV-2 cells supernatants on Neuro-2a cells. BV-2 cells CM was treated by LPS-treated together with isobavachalcone (LPS+Iso group), exerting a protective effect on Neuro-2a cells. And such protective effect was stronger than that of the directly treatment with isobavachalcone plusing the supernatant from LPS-treated BV-2 cells (LPS/Iso group). Means: Control (DMEM basic culture medium), LPS (supernatant from LPS treated BV-2 cells), LPS+Iso (supernatant from LPS plus isobavachalcone treated BV-2 cells), LPS/Iso (isobavachalcone (final concentration 5μM) dissolved in supernatant from LPS treated BV-2 cells). C: Isobavachalcone decreased microglial-induced neuro-2a death in a co-culture system. Bars indicated the mean±SD of three independent experiments. ## p<0.01 in comparison to control group. * p<0.05, ** p<0.01 in comparison to LPS group. △△△ p<0.001 in comparison to LPS+Iso group (supernatant from LPS plus isobavachalcone treated BV-2 cells).</p

    Effects of isobavachalcone on motor, balance and coordination abilities of PD mouse.

    No full text
    <p>Isobavachalcone (Iso, 50 mg/kg) significantly prolonged the residence time of mice on Rota-rod. Bars indicate the mean±SD of three independent experiments. ## p<0.01 indicating very significantly different from Control group. * p<0.05 indicating statistically significantly different from MPTP injury group.</p

    Changes of microglia inflammatory cytokines in vitro and in vivo.

    No full text
    <p><b>A</b>: Isobavachalcone (Iso) significantly inhibited the transcription of IL-6 and IL-1β induced by MPTP injury. B-E: Isobavachalcone inhibited the transcriptional levels of TNF-α, IL-6, IL-1β, and IL-10 induced by LPS in BV-2 cells. F: The expression levels of both TNF-α and IL-6 were decreased with the increasing of the administration isobavachalcone concentration. Bars indicate the mean±SD. ##, p<0.01 vs. respective control group. *,& p<0.05, **p<0.01, ***,&&& p<0.001 vs. PD model or LPS-treated group.</p

    Inkjet-Printed Gold Electrodes on Paper: Characterization and Functionalization

    No full text
    Gold nanoparticles were synthesized and inkjet-printed on a paper substrate and IR-sintered to produce conductive electrodes. The electrodes were further functionalised by using self-assembled octadecanethiol monolayers (SAMs). The effect of sintering, print quality, and SAM formation were examined by topographical, chemical and electrical methods. With optimised printing parameters, a volume resistivity of ∼1.6 ×10<sup>–7</sup> Ω m was attained by a single print layer

    Inkjet-Printed Gold Electrodes on Paper: Characterization and Functionalization

    No full text
    Gold nanoparticles were synthesized and inkjet-printed on a paper substrate and IR-sintered to produce conductive electrodes. The electrodes were further functionalised by using self-assembled octadecanethiol monolayers (SAMs). The effect of sintering, print quality, and SAM formation were examined by topographical, chemical and electrical methods. With optimised printing parameters, a volume resistivity of ∼1.6 ×10<sup>–7</sup> Ω m was attained by a single print layer

    PS Division Notes: January - June 1981

    No full text
    <p>Grain yield and protein concentration of winter wheat as influenced by soil N fertilizer rate and foliar fertilizer applications under field conditions at Yangling during 2010–2014 and Sanyuan during 2012–2014.</p

    Correlation coefficients between nutrients concentrations in grain of winter wheat grown at different soil N fertilizer rates and foliar fertilizer applications at Yangling during 2010–2014 and Sanyuan during 2012–2014.

    No full text
    <p>Correlation coefficients between nutrients concentrations in grain of winter wheat grown at different soil N fertilizer rates and foliar fertilizer applications at Yangling during 2010–2014 and Sanyuan during 2012–2014.</p
    corecore