sj-pdf-1-imr-10.1177_03000605221110699 - Supplemental material for Effectiveness of the PRECEDE-PROCEED model for improving the care knowledge, skill, and sense of competence in mothers of preterm infants

Supplemental material, sj-pdf-1-imr-10.1177_03000605221110699 for Effectiveness of the PRECEDE-PROCEED model for improving the care knowledge, skill, and sense of competence in mothers of preterm infants by Shaoli Li, Shufang Liu, Xinchun Zhang, Yali Chen and Xiaohong Ren in Journal of International Medical Research</p

Prevalence rates of the different genotypes and macrolide resistance mutations by year.

<p>(A) Prevalence of the different P1genotypes from 2003–2015. (B) Prevalence of the different MLVA genotypes from 2003–2015. (C) Prevalence of macrolide resistance mutations from 2003–2015.</p

Relationship between MLVA type, P1 type, and macrolide resistance between 2003 and 2015.

<p>Relationship between MLVA type, P1 type, and macrolide resistance between 2003 and 2015.</p

Genotyping and macrolide resistance mutation results for <i>Mycoplasma pneumoniae</i> in clinical specimens collected from children in Beijing between 2003 and 2015.

<p>Genotyping and macrolide resistance mutation results for <i>Mycoplasma pneumoniae</i> in clinical specimens collected from children in Beijing between 2003 and 2015.</p

Relationship between MLVA type and the presence of macrolide resistance mutations from 2003–2015.

<p>Relationship between MLVA type and the presence of macrolide resistance mutations from 2003–2015.</p

The correlation between time to amplification and amount of target DNA.

<p>The plot reported the fluorescence in millivolts (mV) on the Y-axis and time in minutes on the X-axis. 1, 100 ng/μL; 2, 10 ng/μL; 3, 1 ng/μL; 4, 100 pg/μL; 5, 10 pg/μL; 6, 1 pg/μL; PC, positive control; NC, negative control.</p

Sensitivity and Specificity of the RealAmp assay compared to VITEK 2 system and PCR assay.

*<p>: The species of <i>Acinetobacter</i> determined by 16S rRNA gene sequencing.</p><p>VITEK 2 system: a fluorescence-based automated identification system.</p><p>RealAmp: real-time loop-mediated isothermal amplification.</p><p>PCR: polymerase chain reaction.</p

The RealAmp assay for the detection of <i>A. calcoaceticus–A. baumannii (Ac–Ab)</i> complex.

<p>The plot reported the fluorescence in millivolts (mV) on the Y-axis and time in minutes on the X-axis. 1, <i>Acinetobacter baumannii</i> ATCC19606; 2, <i>Acinetobacter</i> genomic species 13TU; 3, <i>Acinetobacter</i> genomic species 3; 4, <i>Acinetobacter calcoaceticus</i>; PC, positive control; NC, negative control.</p

Additional file 4: of Nomogram integrating gene expression signatures with clinicopathological features to predict survival in operable NSCLC: a pooled analysis of 2164 patients

Prognostic value of the three genomic factors within mutational subsets of NSCLC in the overall pooled dateses, training set and validation set. (XLSX 12 kb

Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Detection of <i>Acinetobacter baumannii</i>

<div><p>Background</p><p>Detection of <i>Acinetobacter baumannii</i> has been relying primarily on bacterial culture that often fails to return useful results in time. Although DNA-based assays are more sensitive than bacterial culture in detecting the pathogen, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. In addition, these molecular tools require expensive laboratory instruments. Therefore, establishing molecular tools for field use require simpler molecular platforms. The loop-mediated isothermal amplification method is relatively simple and can be improved for better use in a routine clinical bacteriology laboratory. A simple and portable device capable of performing both the amplification and detection (by fluorescence) of LAMP in the same platform has been developed in recent years. This method is referred to as real-time loop-mediated isothermal amplification. In this study, we attempted to utilize this method for rapid detection of <i>A. baumannii</i>.</p><p>Methodology and Significant Findings</p><p>Species-specific primers were designed to test the utility of this method. Clinical samples of <i>A. baumannii</i> were used to determine the sensitivity and specificity of this system compared to bacterial culture and a polymerase chain reaction method. All positive samples isolated from sputum were confirmed to be the species of <i>Acinetobacter</i> by 16S rRNA gene sequencing. The RealAmp method was found to be simpler and allowed real-time detection of DNA amplification, and could distinguish <i>A. baumannii</i> from <i>Acinetobacter calcoaceticus</i> and <i>Acinetobacter</i> genomic species 3. DNA was extracted by simple boiling method. Compared to bacterial culture, the sensitivity and specificity of RealAmp in detecting <i>A. baumannii</i> was 98.9% and 75.0%, respectively.</p><p>Conclusion</p><p>The RealAmp assay only requires a single unit, and the assay positivity can be verified by visual inspection. Therefore, this assay has great potential of field use as a molecular tool for detection of <i>A. baumannii</i>.</p></div